Enzyme electrode and enzyme sensor

An enzyme sensor and enzyme electrode technology, applied in the field of enzyme sensors, can solve problems such as lack of stability, inactivation of enzymes, and inability to obtain detection sensitivity and response speed.

Inactive Publication Date: 2009-01-07
株式会社船井电机新应用技术研究所 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] However, in the enzyme sensor described in Non-Patent Document 4, since the carbon nanotubes are coated on the electrodes, a Schottky barrier is formed between the carbon nanotubes and the electrodes, and a large resistance value is measured.
Therefore, it cannot activate the characteristics of carbon nanotubes, and sufficient detection sensitivity and response speed cannot be obtained
[0014] In addition, in the enzyme sensor described in Patent Document 1, since the enzyme is immobilized only at the end of the carbon nanotube, that is, on the surface of the carbon nanotube layer, the characteristics of the carbon nanotube cannot be activated, and high-density immobilization of the enzyme cannot be realized.
Furthermore, in the enzyme sensor described in Patent Document 1, since there is no substance to maintain the three-dimensional structure of the enzyme, the three-dimensional structure of the enzyme will change with the change of the external environment, and the enzyme will lose its activity, and there are problems of lack of stability and short life. question

Method used

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  • Enzyme electrode and enzyme sensor
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  • Enzyme electrode and enzyme sensor

Examples

Experimental program
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Effect test

Embodiment 1

[0128] In Example 1, the enzyme sensor 100 was fabricated by fabricating a base substrate and immobilizing the enzyme 4 on the base substrate to form the enzyme electrode 1 . The enzyme sensor 100 was then evaluated.

[0129] (1) Fabrication of the base substrate

[0130] First, a base substrate is produced.

[0131] (1-1) Fabrication of electrodes

[0132] On the substrate 200, a triode structure of the working electrode (electrode 2), the counter electrode 300, and the reference electrode 400 is patterned.

[0133] Specifically, for example, the substrate 200 made of quartz glass is prebaked at 95° C. for 90 seconds using a hot plate. Afterwards, 50 μL of negative resist was coated by spin coating, and the photomask pattern of the triplet structure of the working electrode (electrode 2 ), the counter electrode 300 and the reference electrode 400 was transferred by an ultraviolet exposure device. Then, post-baking was performed at 120° C. for 60 seconds, development was p...

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Abstract

The invention provides an enzyme electrode capable of detecting aiming objects with high sensitivity, high speed, good stability and long service life, and an enzyme sensor using the enzyme electrode. The enzyme electrode (1) comprises: an electrode (2); a carbon nanotube layer (L) including a plurality of carbon nanotubes (3) extending directly from the electrode (2) and / or a metallic catalyst immobilized on the electrode (2); and an enzyme immobilized in the carbon nanotube layer (L) by being sandwiched between the carbon nanotubes (3).

Description

technical field [0001] The present invention relates to an enzyme electrode and an enzyme sensor using the enzyme electrode. Background technique [0002] In the prior art, research and development of biochemical sensors for detecting specified trace substances are active from the viewpoint of environmental issues and / or applications in the medical field. [0003] Specifically, an enzyme sensor is used, for example, to electrochemically detect a target substance by using an electrode immobilized with an enzyme on a surface. Since the enzyme reacts specifically with the target substance, the enzyme sensor has the characteristic of being able to selectively detect the target substance from the mixture with high sensitivity. As enzyme sensors, for example, a glucose sensor used in diabetes testing, a uric acid sensor used in gout testing, a urea sensor used in renal function testing, and the like have been practically used in the medical field. [0004] In recent years, from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCC12Q1/001B82Y5/00G01N27/3272B82Y15/00C12Q1/00
Inventor 下村威角谷透增田雄一郎小野雅敏
Owner 株式会社船井电机新应用技术研究所
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