Method for carrying out DNA (deoxyribose nucleic acid) detection based on PCR (polymerase chain reaction) assembled magnetic nanoparticles

Abstract

The invention provides a method for carrying out DNA (deoxyribose nucleic acid) detection based on PCR (polymerase chain reaction) assembled magnetic nanoparticles and belongs to the field of nanobiotechnology detection. The method comprises the following steps of: coupling magnetic nanoparticles with an upstream primer and a downstream primer respectively, assembling the magnetic nanoparticles by applying PCR, and detecting a magnetic nanoparticle assembly by adopting a magnetic resonance signal. According to the method for detecting DNA based on the magnetic nanoparticle assembly, magnetic nanoparticles coupled with primers are used, assembling in different degrees is carried out by applying PCR in presence of a target DNA, and the target DNA is detected by the magnetic resonance signal by virtue of difference of aggregation extents of magnetic nanoparticle assemblies. The method for detecting DNA based on PCR-assembled magnetic nanoparticles can realize ultrasensitive detection on DNA, detection limit is low, detection sensitivity is high, specificity is good, and high throughout detection requirement can be realized.

Description

technical field [0001] The invention discloses a method for assembling magnetic nanoparticles based on PCR for DNA detection, which belongs to the field of nanobiological technology detection. Background technique [0002] DNA is the bearer of genetic information and the main material basis of biological inheritance. Each organism has a unique nucleic acid sequence, so the target can be detected by DNA detection of the target. DNA detection is widely used in many fields. In clinical diagnosis, pathogenic bacterial diseases can be detected by detecting the DNA of bacterial and viral pathogens. At present, the sequence information of many diseases has been mastered by people, which can be detected early and accurately. Detect DNA sequences to effectively fight these diseases. In the field of food safety and environmental protection, the detection of harmful microorganisms, genetically modified products and other substances can be realized through the detection of DNA. Theref...

AI Technical Summary

Problems solved by technology

[0003]PCR is a traditional and effective method for detecting and quantifying DNA. However, the products obtained by ordinary PCR need to be analyzed by gel electrophoresis. And the used DNA staining dyes such as EB are highly toxic and have great harm to the human body and the surrounding environment

However, the real-time fluorescent quantitative PCR based on SYBR Green, because the SYBR Green dye can bind to any double-stranded non-specific DNA, thus affecting the final detection and quantification

Images