Double-tagging time resolution fluoroimmunoassay reagent kit based on PSA magnetic particle

A time-resolved fluorescence and immunoassay technology, which is applied in the field of immunoassay analysis technology and nanobiology, can solve the problems of low accuracy of test results, inability to detect tPSA and fPSA at the same time, poor sensitivity and specificity, etc.

Inactive Publication Date: 2016-07-20
JIANGSU INST OF NUCLEAR MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] Therefore, the technical problem to be solved by the present invention is that tPSA and fPSA cannot be detected simultaneously in the prior art, the calculated fPSA/tPSA ratio error is large, the accuracy of the detection result is low, and the detection time is long, the sensitivity and specificity are poor, and the efficiency is low. It ...

Method used

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  • Double-tagging time resolution fluoroimmunoassay reagent kit based on PSA magnetic particle
  • Double-tagging time resolution fluoroimmunoassay reagent kit based on PSA magnetic particle
  • Double-tagging time resolution fluoroimmunoassay reagent kit based on PSA magnetic particle

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Embodiment 1

[0074] This embodiment provides a double-labeled time-resolved fluorescent immunoassay kit based on PSA magnetic particles. Microparticle solution, PSA calibrator solution, europium-labeled fPSA monoclonal antibody solution and samarium-labeled tPSA monoclonal antibody solution, where:

[0075] The europium-labeled fPSA monoclonal antibody solution is Eu 3+ -N 2 -[P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacetic acid-labeled fPSA monoclonal antibody is prepared, and the fPSA monoclonal antibody is combined with the Eu 3+ -N 2 -The mass ratio of [P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacetate is 5:1;

[0076] The samarium-labeled tPSA monoclonal antibody solution is denoted as Sm 3+ -N 2 -[P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacetic acid-labeled tPSA monoclonal antibody is prepared, and the tPSA monoclonal antibody is combined with the Sm 3+ -N 2 -The mass ratio of [P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacet...

Embodiment 2

[0099] This embodiment provides a double-labeled time-resolved fluorescent immunoassay kit based on PSA magnetic particles, including independently packaged reaction buffer, concentrated cleaning solution, enhancement solution, magnetic particle solution coated with PSA monoclonal antibody, PSA calibrator solution, europium-labeled fPSA monoclonal antibody solution and samarium-labeled tPSA monoclonal antibody solution, wherein:

[0100] The europium-labeled fPSA monoclonal antibody solution is Eu 3+ -N 2 -[P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacetic acid-labeled fPSA monoclonal antibody is prepared, and the fPSA monoclonal antibody is combined with the Eu 3+ -N 2 -The mass ratio of [P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacetate is 5:1;

[0101] The samarium-labeled tPSA monoclonal antibody solution is denoted as Sm 3+ -N 2 -[P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacetic acid-labeled tPSA monoclonal antibody is prepared, ...

Embodiment 3

[0124] This embodiment provides a double-labeled time-resolved fluorescent immunoassay kit based on PSA magnetic particles, including independently packaged reaction buffer, concentrated cleaning solution, enhancement solution, magnetic particle solution coated with PSA monoclonal antibody, PSA calibrator solution, europium-labeled fPSA monoclonal antibody solution and samarium-labeled tPSA monoclonal antibody solution, wherein:

[0125] The europium-labeled fPSA monoclonal antibody solution is Eu 3+ -N 2 -[P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacetic acid-labeled fPSA monoclonal antibody is prepared, and the fPSA monoclonal antibody is combined with the Eu 3+ -N 2 -The mass ratio of [P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacetate is 5:1;

[0126] The samarium-labeled tPSA monoclonal antibody solution is denoted as Sm 3+ -N 2 -[P-isocyanic acid-benzyl]-sodium diethylenetriaminetetraacetic acid-labeled tPSA monoclonal antibody is prepared, ...

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Abstract

The invention relates to a double-tagging time resolution fluoroimmunoassay reagent kit based on a PSA magnetic particle and a preparing method and detecting method thereof and belongs to the technical field of immune detection analyzing techniques and nanometer biological techniques. The reagent kit comprises a reaction buffer solution, a concentrated cleaning solution, an enhancing solution, a PSA monoclonal antibody coated magnetic particle solution, a PSA calibrator solution, an europium-labeled fPSA monoclonal antibody solution and a samarium-labeled tPSA monoclonal antibody solution which are independently packaged. The reagent kit can provide a double-tagging reaction system close to a homogeneous phase, can guarantee that the content of tPSA and the content of fPSA can be detected at the same time, enables a detecting result of tPSA and fPSA in the same sample liquid to be high in accuracy and small in error, greatly shortens reaction time, shortens detection time, improves efficiency, improves detection sensitivity and specificity and brings great convenience to clinical application.

Description

technical field [0001] The invention belongs to the field of immunoassay analysis technology and nano-biotechnology, and in particular relates to a double-labeled time-resolved fluorescent immunoassay kit based on PSA magnetic particles and a preparation method and a detection method thereof. Background technique [0002] Prostate specific antigen (PSA) is a serine protease produced by human prostate epithelium. It was first discovered by Hara et al. in 1971 from human seminal plasma and named as seminal plasma protein (gammaseminoprotein). The same glycoprotein was isolated and purified in , and it was proved to be prostate-specific, so it was named prostate-specific antigen. [0003] PSA is a single-chain glycoprotein containing 240 amino acids with a molecular weight of 34KD. Normal prostate epithelium, hyperplastic prostate tissue and prostate cancer cells can all produce PSA. PSA is secreted from the above-mentioned cells and then enters the lumen of the prostate duct...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/573G01N33/543
CPCG01N33/577G01N33/54326G01N33/54346G01N33/573G01N2333/96433G01N2800/342
Inventor 黄飚周彬范俊张艺张珏郭明明邓黎莉朱岚
Owner JIANGSU INST OF NUCLEAR MEDICINE
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