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Novel annular polypeptide ligand used for modifying quantum dots

A cyclic polypeptide and quantum dot technology, applied in the direction of peptides, can solve the problems of poor stability, difficulty in improving binding force, slow binding speed of polypeptide ligands and QDs, etc., achieving high stability, solving insufficient stability, and binding speed. quick effect

Active Publication Date: 2016-10-12
CHANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved in the present invention is: in order to overcome the problems such as slow binding speed and poor stability of existing polypeptide ligands and QDs, limit its popular use in the biological field; Peptide ligands and quantum dots have an impact on self-assembly. Long histidine sequences can enhance binding affinity. However, due to steric hindrance, it is difficult for peptide sequences with more than 6 histidine residues to improve the binding force.

Method used

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  • Novel annular polypeptide ligand used for modifying quantum dots
  • Novel annular polypeptide ligand used for modifying quantum dots
  • Novel annular polypeptide ligand used for modifying quantum dots

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Embodiment 1

[0020] The method of the present invention adopts the conventional solid-phase Fmoc method, that is, the monomer amino acid protected by Fmoc on the solid-phase resin is deprotected to expose the amino group, and forms a peptide bond with the carboxyl group of the amino acid in the solution through a condensation reaction, thereby linking the amino acid to the On the resin, the peptide chain is extended from the C-terminus to the N-terminus.

[0021] 1. Basic materials:

[0022] (1) Resin and linking molecule: The resin selected by the solid-phase Fmoc method is Rink Amide- resin. This kind of resin has very good swelling property, which can make the condensation reaction between the peptide chains better, and there is enough network space to meet the growing peptide chains. HBTU and HOBt are used as linking molecules to immobilize the polypeptide molecules on the resin.

[0023] (2) Monomer: The monomer used in the synthesis is a chemically modified α-amino acid.

[0024...

Embodiment 2

[0046]Peptide synthesis steps refer to Example 1, according to HCHLH D P L The order of PHLHCH sequences was synthesized from right to left. Synthesis of a Novel Cyclic Peptide Ligand Sequence: ATTO-HCHLH D P L PHLHCH.

Embodiment 3

[0048] Peptide synthesis steps refer to Example 1, according to HCHVH D P L The order of PHVHCH sequences was synthesized from right to left. Synthesis of a novel cyclic polypeptide ligand sequence: ATTO-HCHVH D P L PHVHCH

[0049] According to the method of Example 1, the peptide sequences obtained in Examples 2-3 were analyzed with QDs by capillary electrophoresis, and the detection conditions were the same as in Example 1. The test results showed that they could all be stably combined with QDs.

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Abstract

The invention discloses a novel annular polypeptide ligand (ATTO-H6) used for modifying quantum dots, and belongs to the technical field of nano-biology. The novel annular polypeptide ligand comprises a dye ATTO 590 (1) used for undergoing FRET together with quantum dots, a sequence (2) used for polypeptide cyclization, histidine intervening sequences (3) used for combining the quantum dots, and sequences (4) used for polypeptide folding, and all sequences are combined through peptide bonds. The ligand is simple to synthesize, and the combination of six histidine intervening sequences and the quantum dots greatly improves the combination rate and stability of the ligand and the quantum dots, so the ligand can be widely used in biological marking as a fluorescence nano-probe.

Description

technical field [0001] The invention relates to the field of nanobiology technology, in particular to a novel cyclic polypeptide ligand for modifying quantum dots. Background technique [0002] Compared with traditional organic dyes, quantum dots (QDs) have more excellent spectral properties, such as wide and continuous distribution of excitation spectrum, and symmetrical distribution of emission spectrum with narrow width, adjustable color, and high photochemical stability, not easy to light Solution (Marcel BJ, Mario M, Peter G, et al. Science 1998, 281, 2013-2016). These superior properties make QDs widely used in in vivo cell imaging and biological specific labeling. It is and will increasingly become a very important probe tool in molecular cell imaging research. Therefore, the synthesis of water-soluble quantum dots with high performance has received close attention. [0003] The development of QDs-based sensors is highly dependent on novel ligands and their self-ass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08
CPCC07K7/08
Inventor 王建浩张晨澄邱琳蒋鹏举柳丽王车礼滕一万陈瑶
Owner CHANGZHOU UNIV
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