The invention discloses a
gene mutation multi-detecting method based on a
signal amplification DNA logic gate. The method comprises the following steps: performing high specificity recognition on a detecting target by invading into a
signal amplification reaction by utilizing
nucleic acid, and simultaneously, invading into the
signal amplification reaction by utilizing a controllable
DNA polymerase extension reaction bridging two-step
nucleic acid to form
signal amplification. The method disclosed by the invention has high detecting sensitivity and high detecting specificity to a
nucleic acid target.
A DNA logic gate is constructed by invading into the
signal amplification reaction by adopting the controllable
DNA polymerase extension reaction bridging two-step nucleic acid disclosed by the invention, a background signal is inhibited by introducing an
intervening sequence, and meanwhile, a two-step
signal amplification reaction exists, so that the defecting sensitivity is improved, and high signal-to-
noise ratio output can be realized under the conditions of trace amount of
DNA input and short reaction time. Moreover, through the method established by the invention, a plurality of
gene mutation points can be detected simultaneously and results can be subjected to logical judgment, and an analyzing process of
gene mutation detection in a clinically individualized medicating process is simplified.