Antigen-antibody detection method

A detection method, antigen-antibody technology, applied in biological testing, material inspection products, etc., can solve problems such as lack of versatility

Pending Publication Date: 2021-12-10
FOSHAN MICROWONDERS BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the current chip / electrode lacks versatility because of the inconsistency in process and materials, and the frequency points that characterize the specific binding of substances are different when used for accelerated detection. Therefore, it is difficult to determine the universal excitation signal according to the characteristic points

Method used

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  • Antigen-antibody detection method

Examples

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Embodiment 1

[0053] An antigen-antibody detection method of the present embodiment comprises the following steps:

[0054] The steps for determining the frequency band of the characteristic response include:

[0055] Determine the characteristic response frequency band of the same preset type of chip through different background liquids:

[0056] S110. Clean the chip with the background solution A for a first preset time. In this embodiment, the first preset time is more than 30 seconds, then add the background solution A to the chip, perform the first frequency scan, and obtain the first curve. In this embodiment, the frequency scan is a complex impedance frequency scan;

[0057] S120. Immediately remove the background liquid A in the chip after scanning and wash it with water for a first preset time. In this embodiment, the water is ultrapure water. Then use the background solution B to wash for the first preset time, and then add the background solution B after the cleaning is complet...

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Abstract

The invention relates to the technical field of immunoassay detection, and particularly discloses an antigen-antibody detection method which comprises the following steps: a characteristic response frequency band determination step: determining a characteristic response frequency band F1 of a chip of a preset type through different background solutions, and determining a characteristic response frequency band F2 caused by a sample through a sample solution; a selection step: judging whether the characteristic response frequency band F1 and the characteristic response frequency band F2 coincide or not; and a detection step: if coincidence exists, adding a sample on the chip, selecting a specific frequency band based on coincidence under a specific voltage, carrying out measurement under an acceleration condition, and determining the content of a target object in the sample or carrying out qualitative judgment according to the change rate of an electric signal value obtained by measurement. By adopting the method provided by the invention, the universality of chip immunodetection can be improved.

Description

technical field [0001] The invention relates to the technical field of immunoassay and detection, in particular to an antigen-antibody detection method. Background technique [0002] Immunoassay is a technique for qualitative or quantitative analysis of specific biochemical substances based on antigen-antibody binding. Antibodies recognize and bind to the corresponding antigen through the epitope on the surface of the antigen. This recognition also makes immune detection highly specific: for example, AIDS antibodies will only bind to AIDS antigens and will not react with other antigens. [0003] In the traditional detection method, the combination of the target antibody or antigen and the corresponding antigen or antibody in the kit depends on molecular diffusion motion and random Brownian motion. The whole process is very passive, so the detection time is long, from collecting samples to getting results It often takes tens of minutes to several hours. [0004] In order t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53
CPCG01N33/53
Inventor 刘晓竹徐海李俊胡勇杨黎华马良童立林杰宋娜
Owner FOSHAN MICROWONDERS BIOTECHNOLOGY CO LTD
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