Preparation method of magnetic separation fluorescence quantitative detection kit of aflatoxin B1

A fluorescence quantitative detection, aflatoxin technology, applied in fluorescence/phosphorescence, measurement devices, analytical materials, etc., can solve the problem of inability to perform quantitative detection

Inactive Publication Date: 2010-12-22
JILIN UNIV
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Problems solved by technology

At present, the method of rapid detection with nanomaterials is to use gold standard test paper for detection. This met

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  • Preparation method of magnetic separation fluorescence quantitative detection kit of aflatoxin B1
  • Preparation method of magnetic separation fluorescence quantitative detection kit of aflatoxin B1
  • Preparation method of magnetic separation fluorescence quantitative detection kit of aflatoxin B1

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Embodiment Construction

[0017] 1. Preparation of magnetic particles:

[0018] (1) Referring to the document J.Am.Chem.Soc., 2004, 126, 273-279, Fe is synthesized by pyrolysis method 3 o 4 magnetic particles. to N 2 Add iron acetylacetonate [Fe(acac) 3 ] (0.3532g), 1,2-dodecanediol (1.0117g), oleic acid (1.0220mL), oleylamine (0.99mL) were mixed, benzyl ether (10mL), magnetically stirred. The mixture was heated to 200°C for 2h; then the nitrogen flow was stopped, and then heated to about 300°C for 1h, the heating was stopped to terminate the reaction, and cooled to room temperature. After cooling, add 20mL ethanol to the three-necked flask, centrifuge at 14000rpm for 20min, discard the supernatant; disperse the precipitate in a mixed solution of 25uL oleic acid, 25uL oleylamine and 5mL n-hexane; centrifuge at 6000rpm for 10min; discard the precipitate, and add 10mL ethanol to the supernatant , centrifuged at 10000rpm for 20min, and discarded the supernatant to obtain Fe 3 o 4 Magnetic particles...

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Abstract

The invention belongs to the technical field of nanobiology, particularly relating to a preparation method of a magnetic separation fluorescence quantitative detection kit of aflatoxin B1. The invention is a measuring method which combines the quick separation technology of an immunological detection system and magnetic nanometer material with the lightening characteristics. Firstly, magnetic isolating reagent, namely compound (I) is prepared; then, aflatoxin B1 is coupled to the compound (I); fluorescent particle solution (compound (II)) coupled with holoantigen is added; the aflatoxin B1 in the compound (II) and residual antibody on the compound (I) generate immune response to form the immunological sandwich compound (III) of the compound (I) and compound (II); and thus, the density of aflatoxin is in inverse proportion to the fluorescence intensity of the fluorescence particle solution of the final detected solution. The invention has the advantages of quick, accurate and obvious detection, high sensitivity and the like.

Description

technical field [0001] The invention belongs to the field of nano-biotechnology, in particular to a kind of aflatoxin B 1 The preparation method of the immunomagnetic separation fluorescent quantitative detection kit. Background technique [0002] Aflatoxin (AF for short) is a secondary metabolite mainly produced by Aspergillus flavus and Aspergillus parasiticus, which is found in corn, wheat, sorghum, sweet potato, soybean, cottonseed, poultry eggs, meat, milk and dairy products all over the world. There was aflatoxin contamination in all of them. [0003] Among the 18 aflatoxins discovered so far, aflatoxin B 1 (AFB 1 ) is the most toxic mycotoxin, its toxicity is 68 times that of arsenic, and its ability to induce liver cancer is 75 times that of dimethylnitrosamine. One of the strongest substances. AFM 1 、AFB 2 、AFG 1 next. At the same time, AF is very stable and can be completely destroyed when heated to 230°C, so it is not easy to eliminate in general cooking ...

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Application Information

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IPC IPC(8): G01N33/532G01N21/64
Inventor 徐力张代辉郭轶张学忠吕绍武
Owner JILIN UNIV
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