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Construction method of chiral sensor for detecting DNA enzymatic assembly of lead ion

A DNA enzyme and construction method technology, applied in the field of nano-biotechnology detection, can solve the problems of complex sample pretreatment, expensive instruments, time-consuming and so on

Inactive Publication Date: 2014-04-02
JIANGNAN UNIV
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Problems solved by technology

[0003] Traditional Pb 2+ Detection methods include inductively coupled plasma (ICP) and atomic absorption spectrometry. These methods have the advantages of high sensitivity and accurate quantification, but the required instruments are expensive, sample pretreatment is complicated and time-consuming, and the detection personnel are required to have certain professional skills. Knowledge, high cost of analysis

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  • Construction method of chiral sensor for detecting DNA enzymatic assembly of lead ion
  • Construction method of chiral sensor for detecting DNA enzymatic assembly of lead ion

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Embodiment 1

[0023] Determination of Pb 2+ Construction method of DNase-assembled chiral sensor. Including: 10nm and 20nm silver nanoparticles are respectively modified with a pair of DNA probes, and the silver nanoparticles are assembled into an asymmetric chiral dimer under the action of DNase, and the silver nanoparticle assembly is used as a detection sensor for Pb 2+ For the detection, the CD spectrum is used for detection; the specific steps are:

[0024] (1) Silver nanoparticles of 10nm and 20nm were modified with a pair of DNA probes respectively

[0025] Concentrate the newly synthesized 10nm and 20nm silver nanoparticles by centrifugation at 10000r / min and 8000r / min respectively, and then resuspend the silver nanoparticles into 10mM Tris-HCl buffer containing 50mM NaCl , so that the final concentrations of 10nm and 20nm silver nanoparticles were 50 nM and 20 nM respectively; then DNA probes and silver nanoparticles were coupled at a molar ratio of 10:1, specifically as follows:...

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Abstract

The invention provides a construction method of a chiral sensor for detecting DNA enzymatic assembly of a lead ion, belonging to the field of nano-biotechnology detection. The method comprises the steps of modifying a silver nano particle of 10nm and a silver nano particle of 20nm with a pair of DNA probes respectively, and assembling the modified silver nano particles to form an asymmetrical chiral dimer under the action of DNA enzyme, wherein the silver nano particle assembly is taken as a detecting sensor for detecting Pb<2+> by circular dichroism (CD). The construction method is used for detecting the Pb<2+> through a CD signal by the means that the big silver nano particle and the small silver nano particle are assembled to form the asymmetrical chiral dimmer under the action of the Pb<2+>-dependent DNA enzyme and by virtue of the silver nano particle probe. The method can be used for performing ultra-sensitive detection on the Pb<2+>, is high in detecting sensitivity and good in specificity, and is capable of detecting a trace target object in a water solution.

Description

technical field [0001] A method for the determination of Pb 2+ The invention relates to a method for constructing a chiral sensor assembled by DNA enzyme, and belongs to the field of nanometer biotechnology detection. Background technique [0002] Pb 2+ It is one of the most toxic heavy metal ions. Lead pollution comes from production wastewater and wastes such as mining, smelting, rubber production, dyes, printing, ceramics, lead glass, solder, cables and lead pipes. In addition, tetraethyl lead in automobile exhaust is a highly toxic substance. With the rapid development of industry and agriculture in our country, the pollution of heavy metals in the environment is becoming more and more serious. The poison caused by heavy metal pollution persists and will enter the food chain with the recirculation of soil and water, posing a threat to food safety and endangering human life and health. So far, lead has no known physiological role in living organisms. Any lead in human...

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Application Information

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IPC IPC(8): G01N21/76G01N1/28
Inventor 徐丽广胥传来尹红红匡华刘丽强马伟宋珊珊
Owner JIANGNAN UNIV
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