Method for detecting bisphenol A by utilizing aptamer-based chiral sensor

An aptamer and sensor technology, applied in the field of nanobiotechnology detection

Active Publication Date: 2014-04-30
JIANGNAN UNIV
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  • Abstract
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Problems solved by technology

Although these methods have the characteristics of good stability, high sensitivity, and accurate quantitative and qualitative detection, they require expensive instruments, complex sample pretreatment processes, and professional operators. Immunological detection methods rely on antigen-antibody recognition reactions. However, the stability of the antibody and the specificity of the experimental results are the main factors affecting the detection results

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  • Method for detecting bisphenol A by utilizing aptamer-based chiral sensor
  • Method for detecting bisphenol A by utilizing aptamer-based chiral sensor

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Embodiment 1

[0021] An aptamer-based chiral sensor detection method for bisphenol A, the steps are:

[0022] (1) Gold nanoparticle modified DNA probe

[0023] Firstly, the newly synthesized 10nm and 20nm gold nanoparticles are concentrated 5 times to make the final concentration of 20 nM and 5 nM respectively, and then the gold nanoparticles and DNA are coupled according to the molar concentration ratio of 1:5, that is, 1 μL 2.5μM BPA aptamer (Aptamer) was modified on the surface of 20nm gold nanoparticles, 1 μL 10μM BPA aptamer partial complement sequence (Aptamer-C) was modified on the surface of 10nm gold nanoparticles; aged by adding salt Methods Gradually add NaCl to the gold nanoparticles to make the final concentration of NaCl reach 50 mM; after overnight incubation, the gold nanoparticles are centrifuged to remove uncoupled DNA;

[0024] Aptamer: 5’-SH-CCGGTGGGTG GTCAGGTGGG ATAGCGTTCC GCGTATGGCC CAGCGCATCA CGGGTTCGCA CCA-3’;

[0025] Aptamer-C: 5'-CCCACCTGAC CACCCACCGG-SH-3'.

[0026] (2) ...

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Abstract

The invention relates to a method for detecting bisphenol A (which is abbreviated as BPA) by utilizing an aptamer-based chiral sensor and belongs to the field of the detection of nano biological technology. The method comprises the following steps of modifying a DNA (deoxyribonucleic acid) probe by utilizing gold nanoparticles, assembling an asymmetric dipolymer through the gold nanoparticles, and detecting the BPA through the gold nanoparticle assembly by utilizing a circular dichroism (CD). The method has the beneficial effects that the gold nanoparticle probe modified by BPA aptamer is applied, on the basis of alkali-base complementary pairing principle, the aptamer and the gold nanoparticles modified by the complementary sequence are assembled to form the chiral dipolymer under the hybridization effect of the DNA, and the BPA is detected through the difference of CD signals under different BPA concentrations. The method is simple, fast, high in sensitivity, good in specificity and capable of detecting the real sample.

Description

Technical field [0001] A method for detecting bisphenol A by a chiral sensor based on an aptamer belongs to the field of nanobiotechnology detection. Background technique [0002] Bisphenol A (abbreviation BPA) is an important organic compound. In industrial production, bisphenol A is widely used as a raw material for the production of a variety of polymer materials, such as: polysulfone resin, polyphenylene ether resin, unsaturated Polyester resin, epoxy resin, polycarbonate, etc.; it can also be used in the production of fine chemicals, such as plasticizers, heat stabilizers, rubber antioxidants, flame retardants, antioxidants, coatings, pesticides, etc., at the same time It is also an "environmental endocrine disruptor". The residues of BPA in food are mainly through two ways of food raw materials and food packaging. Path 1: BPA is difficult to degrade in the environment, widely exists in nature, and is enriched in organisms, and enters our food through the food chain. ; App...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N21/25
CPCG01N21/25G01N33/53
Inventor 匡华胥传来尹红红马伟徐丽广宋珊珊刘丽强
Owner JIANGNAN UNIV
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