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Method for detecting bisphenol A by utilizing aptamer-based chiral sensor

An aptamer and sensor technology, applied in the field of nano-biotechnology detection

Active Publication Date: 2015-07-08
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods have the characteristics of good stability, high sensitivity, and accurate quantitative and qualitative detection, they require expensive instruments, complex sample pretreatment processes, and professional operators. Immunological detection methods rely on antigen-antibody recognition reactions. However, the stability of the antibody and the specificity of the experimental results are the main factors affecting the detection results

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  • Method for detecting bisphenol A by utilizing aptamer-based chiral sensor
  • Method for detecting bisphenol A by utilizing aptamer-based chiral sensor

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Embodiment 1

[0021] A method for detecting bisphenol A based on an aptamer-based chiral sensor, the steps are:

[0022] (1) Gold nanoparticles modified DNA probes

[0023] Firstly, the newly synthesized 10nm and 20nm gold nanoparticles were concentrated 5 times to make the final concentrations of 20 nM and 5 nM respectively, and then the gold nanoparticles and DNA were coupled according to the molar concentration ratio of 1:500, that is, 1 μL 2.5 μM BPA aptamer (Aptamer) was modified to the surface of 20 nm gold nanoparticles, and 1 μL of 10 μM BPA aptamer partial complementary sequence (Aptamer-C) was modified to the surface of 10 nm gold nanoparticles; Methods NaCl was gradually added to the gold nanoparticles, so that the final concentration of NaCl reached 50 mM; after overnight incubation, the gold nanoparticles were centrifuged to remove uncoupled DNA;

[0024] Aptamer: 5'-SH-CCGGTGGGTG GTCAGGTGGG ATAGCGTTCC GCGTATGGCC CAGCGCATCA CGGGTTCGCA CCA-3';

[0025] Aptamer-C: 5'-CCCACCTGAC...

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Abstract

The invention relates to a method for detecting bisphenol A (which is abbreviated as BPA) by utilizing an aptamer-based chiral sensor and belongs to the field of the detection of nano biological technology. The method comprises the following steps of modifying a DNA (deoxyribonucleic acid) probe by utilizing gold nanoparticles, assembling an asymmetric dipolymer through the gold nanoparticles, and detecting the BPA through the gold nanoparticle assembly by utilizing a circular dichroism (CD). The method has the beneficial effects that the gold nanoparticle probe modified by BPA aptamer is applied, on the basis of alkali-base complementary pairing principle, the aptamer and the gold nanoparticles modified by the complementary sequence are assembled to form the chiral dipolymer under the hybridization effect of the DNA, and the BPA is detected through the difference of CD signals under different BPA concentrations. The method is simple, fast, high in sensitivity, good in specificity and capable of detecting the real sample.

Description

technical field [0001] The invention discloses a method for detecting bisphenol A based on an aptamer-based chiral sensor, which belongs to the field of nanobiological technology detection. Background technique [0002] Bisphenol A (abbreviated as BPA) is an important organic compound. In industrial production, bisphenol A is widely used as a raw material for the production of various polymer materials, such as: polysulfone resin, polyphenylene ether resin, unsaturated Polyester resin, epoxy resin, polycarbonate, etc.; can also be used in the production of fine chemicals, such as: plasticizers, heat stabilizers, rubber antioxidants, flame retardants, antioxidants, coatings, pesticides, etc., while It is also an "environmental endocrine disruptor". Bisphenol A residues in food are mainly through two ways: food raw materials and food packaging. Way 1: Bisphenol A is difficult to degrade in the environment, widely exists in nature, enriches in organisms, and enters our food th...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCG01N21/25G01N33/53
Inventor 匡华胥传来尹红红马伟徐丽广宋珊珊刘丽强
Owner JIANGNAN UNIV
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