Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing carbon nanotube-protein stationary phase

A technology for immobilizing carbon nanotubes and proteins, which is applied in the nanometer field, can solve problems not involved in the application of microfluidic chips, and achieve the effects of increasing the amount of immobilization, good stability, and high activity

Inactive Publication Date: 2006-07-19
FUDAN UNIV
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are domestic patents (patent application number: 99113091.X, publication number: CN 1280986A) reporting the use of protein as a chiral stationary phase in chromatography, but it does not involve the application in microfluidic chips

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing carbon nanotube-protein stationary phase
  • Method for preparing carbon nanotube-protein stationary phase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment one. the preparation method I of protein stationary phase

[0030] (1) Purified carbon nanotubes

[0031] Put 1g of SWNTs in a round bottom flask, add 16ml concentrated HNO 3 , reflux in an oil bath at 140°C for 4 hours, dilute with water, centrifuge, discard the supernatant, repeat washing and centrifugation until the supernatant pH ~ 6, filter with a microporous membrane with a pore size of 0.22 μm to obtain a black solid, dry for later use .

[0032] (2) Truncated nanotubes

[0033] Take 50 mg of the above-mentioned purified SWNTs in 45 ml of concentrated HNO 3 and concentrated H 2 SO 4 Sonicate in (1:3v / v) for 8 hours, dilute, filter, wash the solid until the filtrate is close to neutral, and dry the solid for later use.

[0034] (3) Activation of nanotubes and covalent binding of proteins

[0035] Weigh 0.075 mg of the cut-off SWNTs above, add 1 ml PBS, sonicate for 5 minutes, add 0.4 mg EDAC and sonicate for 2 hours, continue to add 0.9 mg BSA, a...

Embodiment 2

[0036] Embodiment two. The preparation method II of protein stationary phase

[0037] The difference from the preparation method I of the protein stationary phase is that the above cut-off 0.075mg SWNTs was added to 1ml PBS, sonicated for 5 minutes, 0.3mg EDC and 0.75mg NHS were added and sonicated for 2 hours, and 0.9mg BSA was added, and stirred for 24 hours Then the protein stationary phase is obtained.

Embodiment 3

[0038] Example three. Separation of tryptophan enantiomers.

[0039] Using the BSA stationary phase prepared in the above example, use a micro-sampler to fill the PMMA chip modified with polymers, place it in a refrigerator at 4°C to dry, use phosphate buffer as the mobile phase, and tryptophan enantiomers can be separated . The separation conditions are as follows: the separation channel length of the chip is 3.2 cm; the separation field strength is +218 V / cm; 20 mM phosphate buffer solution, pH=7.4; 24 μM tryptophan; and the voltage is 0.6 V for constant potential detection. figure 2Separation of tryptophan enantiomers is shown. It can be seen that tryptophan isomers can be baseline-separated within 70 seconds in the chip channel with BSA protein stationary phase, and the resolution is about 1.28. This result shows that the protein stationary phase prepared according to the invention can not only be stably fixed in the channel of the chip, but also preserve the original c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The preparation method for chiral separating stationary phase with functional nano carbon tube bonded protein as microflow chip overcomes the defect of bad effect and unstable performance led by unstable chip electroosmosis, can be applied to modified chip effectively by means of the fixed phase in chip separation channel, and has very high success rate. Wherein, the fixed protein has high activity and well stability.

Description

technical field [0001] The invention relates to the fields of nano, biotechnology and micro total analysis, and provides a preparation method that can be used as a chiral stationary phase of a microfluidic chip. Background technique [0002] Chiral research plays an important role in the fields of life sciences, new materials, and new drugs. Among them, chiral separation has always been the focus and difficulty in this field. Over the years, chromatography and chiral capillary electrophoresis have become relatively mature modes for the separation of chiral isomers (Wan, H., Blomberg, L.G., J. Chromatogr. A2000, 875, 43-88). In recent years, with the development of Micro Total Analysis System (μ-TAS), chiral separation based on microfluidic chip has attracted more and more attention due to its advantages of fast, efficient, low sample consumption and high integration. In these studies, cyclodextrin and its derivatives (Belder, D., Ludwig, M., Electrophoresis 2003, 24, 2422-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K17/14B01J20/29
Inventor 孔继烈刘宝红翁雪香毕红艳
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com