Biological cell ultrasonic atomic force microscopic detection system and method

Abstract

The invention relates to a biological cell ultrasonic atomic force microscopic detection system and method under the physiological environment. The system is composed of a detector, a piezoelectric transducer, a probe control module, a phase-locked amplifier and an ultrasonic ranging module. The detector is arranged in the physiological environment to scan biological cells, and the piezoelectric transducer vibrates at ultrasonic frequency. Ultrasonic signals penetrate through the biological cells to generate different signal responses. The system acquires the response signals so as to obtain the biological cell morphology and internal maps. The ultrasonic ranging module records and processes echo delay signals and quantitatively expresses an acoustic path of a vertical direction so as to reconstruct the depth map of the biological cells. Nanoscale ultra-micro internal structural analysis of the biological cells can be realized, a probe can be accurately located and guided to inject drug loading nanoparticles into cancer cells and the stress changes of the morphology and the internal structure can be observed so that the system and the method can be used for nanoscale lossless measurement of the living cells under the physiological environment and have a directive significance for nano-biotechnology and nano-medicine.

Description

technical field [0001] The invention relates to a biological cell ultrasonic atomic force microscopic detection system and method, and is especially suitable for the non-destructive measurement of the nanoscale internal structure of cells in a physiological environment. Background technique [0002] With the development of nanotechnology, atomic force microscopy (AFM) has become one of the most important nanotechnology. Because of its wide working range, its application prospect is extremely wide, especially in the field of biomedicine, which has important research value. [0003] At the nanoscale, the properties and behaviors of biological samples and materials are not only related to their surface structures, but also to their internal structures. In today's technology, transmission electron microscope (TEM) is the main measurement technique for imaging internal structures. When measuring, TEM needs to cut the sample to the thickness that electrons can penetrate, generall...

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Problems solved by technology

When measuring, TEM needs to cut the sample to the thickness that electrons can penetrate, generally only tens of nanometers, so the cells need to be sliced, which will also destroy the cells

In 2009, De Jonge et al. of the University of Southern California successfully imaged the submicroscopic structure of COS7 fibroblasts in a liquid environment using scanning transmission electron microscopy (STEM). Metal layer, which is a destructive measurement

In 2009, Zhang Bo, Cheng Qian and others from Tongji University combined the atomic force microscope with a self-made frequency conversion acoustic excitation platform to study the acoustic image characteristics of rat aortic smooth muscle cells. characteristic

[0004] Due to the low rigidity of cells, and the difficulty in conducting nanoscale nondestructive testing of biological living cells in physiological environments, especially for internal structure detection and imaging, there is still a lack of effective research methods to achieve nanoscale ultrafine internal structure analysis methods.

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