Method for marking immune globulin by quantum dot

A technology of immunoglobulin and quantum dots, which is applied in the field of quantum dot-labeled immunoglobulins, can solve the problems of large loss of immunoglobulins, reduced activity, and low efficiency, and achieves simple operation and use of equipment, improved utilization, and high activity. high effect
CN103487574AActive Publication Date: 2014-01-01SHENZHEN KINGFOCUS BIOMDICAL ENG CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENZHEN KINGFOCUS BIOMDICAL ENG CO LTD
Publication Date
2014-01-01

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Abstract

The invention belongs to the field of a nano-biotechnology and particularly relates to a method for marking immune globulin by a quantum dot. The method comprises the following steps: washing the quantum dot, activating, washing the quantum dot for the second time, washing the immune globulin, coupling and separating. According to the method, structural characteristics of the water-soluble quantum dot, the reaction characteristics of EDC (1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) activated carboxyl and the reaction characteristics of a condensation reaction are utilized; the carboxyl on the surface of the quantum dot is activated sufficiently by optimizing conditions of all the steps and is in a suitable marking environment; the quantum dot can be combined with the surface of the immune globulin by the condensation reaction through optimizing the condition of the coupling step, and the marking efficiency is high. The immune globulin which is not reacted can be recycled to be marked continually through exclusion chromatography, separation and purification by a reaction solution obtained by the coupling step so that the utilization rate of the immune globulin is improved and the cost is saved. According to the method provided by the invention, the activity of a prepared protein-quantum dot conjugate is high, the operation and used equipment are simple, and the production can be enlarged industrially.
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Description

technical field

[0001] The invention relates to the field of nanobiology technology, in particular to a method for marking immunoglobulin with quantum dots. Background technique

[0002] Quantum dots (QDs) are aggregates composed of a certain number of actual atoms, and semiconductor compounds with three-dimensional dimensions less than 100nm. Quantum dots have many advantages such as high luminous intensity, wide excitation spectrum, narrow emission spectrum, long fluorescence lifetime, multifunctional surface modification and good stability. They have the potential to replace traditional organic fluorescent dyes in the field of fluorescence detection and have become a new generation of biological Fluorescent markers. In various immunological experiments, the method of tracking proteins and detecting protein content by labeling fluorescent molecules on proteins has been widely used.

[0003] In 2009, Zhang Guohua et al. published a method for labeling ractopamine antibodi...

Claims

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