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Construction method of ultra-sensitive surface-enhanced Raman spectrum (SERS) sensor for measuring Hg<2+>

A surface-enhanced Raman and spectral sensor technology, applied in the field of nano-biotechnology detection, can solve problems such as expensive, complex and time-consuming, and limited universal application.

Active Publication Date: 2014-07-30
JIANGNAN UNIV
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Problems solved by technology

Although these methods can be used for Hg 2+ Perform accurate detection, but expensive instruments, complex and time-consuming sample pretreatment procedures and professional operator requirements limit the general application of this method

Method used

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  • Construction method of ultra-sensitive surface-enhanced Raman spectrum (SERS) sensor for measuring Hg&lt;2+&gt;
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Embodiment 1

[0020] A measure of Hg 2+ The construction method of ultrasensitive surface-enhanced Raman spectroscopy sensor, 15nm gold nanoparticles were used to capture Hg 2+ Sequence modification of two aptamers, AuNPs in Hg 2+ Assemble into a chain structure under the condition of existence, and the chain structure of gold nanoparticles is determined by surface-enhanced Raman spectroscopy (SERS); the specific steps are:

[0021] (1) 15nm gold nanoparticles were used to capture Hg 2+ The two aptamer sequence modification ：

[0022] The newly synthesized 15nm gold nanoparticles were concentrated tenfold by centrifugation at 10,000r / min, and then resuspended in 0.01 M Tris-HCl buffer to a final concentration of 20nM for DNA Coupling of DNA; DNA coupling was performed in a 100 μL reaction system, 2 μL of 4 μM DNA1 and DNA2 were added to 100 μL of 20 nM gold nanoparticle solution, so that the final concentration of DNA was 80 nM, DNA and gold nanoparticles at 4 : 1 molar ratio for coupl...

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Abstract

The invention relates to a construction method of an ultra-sensitive surface-enhanced Raman spectrum (SERS) sensor for measuring Hg<2+>, belonging to the technical field of nano-biotechnology detection. According to the method, gold nanoparticles with length of 15nm are respectively modified by two aptamer sequences for capturing Hg<2+> and are assembled to form chain structures in the presence of Hg<2+>, and the chain structures are measured by virtue of an SERS. By virtue of high selectivity and capturing capacity of double-stranded DNA consisting of mismatched bases of thymine-thymine (T-T) to Hg<2+>, the gold nanoparticles are assembled to form the chain structures with different lengths, and Hg<2+> is detected by virtue of SERS signals. The method is high in Hg<2+> detection sensitivity, good in specificity and high in practicability and is an ideal ultra-sensitive detection method for trace Hg<2+>.

Description

technical field [0001] The invention relates to a method for measuring Hg 2+ The invention discloses a method for constructing an ultrasensitive surface-enhanced Raman spectroscopy sensor, belonging to the technical field of nanobiotechnology detection. Background technique [0002] Mercury is a ubiquitous environmental pollutant, and all its oxidation states are highly toxic. The pollution sources mainly come from anthropogenic mercury emissions and the natural environment. At the same time, mercury is also a cumulative pollutant, which can accumulate in important tissues and organs of the human body, thereby causing serious harm to human health, especially the central nervous system, respiratory system, kidneys, heart, etc. caused by mercury poisoning. and muscle damage. Hg in drinking water set by the US Environmental Protection Agency 2+ The maximum allowable limit is 2ppb, so the development of highly sensitive Hg 2+ The detection method is particularly important. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 徐丽广胥传来尹红红匡华马伟刘丽强宋珊珊吴晓玲
Owner JIANGNAN UNIV
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