Double-labeling kit for detecting troponin (Tn) and compound and preparation and detection methods of double-labeling kit

A technology of troponin and complexes, which is applied in the field of immunoassay analysis technology and nanobiology, can solve the problems of increase and long duration, achieve long storage time, wide range of standard curve, and improve detection accuracy

Pending Publication Date: 2020-02-25
浙江博实生物科技有限公司
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Problems solved by technology

Although the half-life of troponin is very short (cTnT 2 hours, free cTnI 2h~5d), its degradation pr

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  • Double-labeling kit for detecting troponin (Tn) and compound and preparation and detection methods of double-labeling kit
  • Double-labeling kit for detecting troponin (Tn) and compound and preparation and detection methods of double-labeling kit
  • Double-labeling kit for detecting troponin (Tn) and compound and preparation and detection methods of double-labeling kit

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Embodiment Construction

[0040] Below in conjunction with accompanying drawing and embodiment the present invention is described in further detail:

[0041] As an embodiment of the double-labeled kit and preparation and detection method for detecting troponin and complexes described in the present invention, the reaction principle is as follows: Figure 1-2 , an antibody against the cTnI region protected by endogenous cTnC serves as a solid-phase antibody to capture all cTnI, an antibody against cTnC is labeled Eu for detection of cTnI-C complexes, and an antibody against the end of cTnI is labeled with Sm , used to detect the complete fragment of cTnI, because the end of cTnI is easily hydrolyzed after entering the blood, so that the complete cTnI is reduced, and the cTnI-C complex exists more stably, so that the complete cTnI and cTnI-C complex can be detected , and calculating its ratio will be helpful in judging the duration of the onset of AMI.

[0042] In this embodiment, the double-labeled t...

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Abstract

The invention relates to a double-labeling kit for detecting troponin (Tn) and a compound and preparation and detection methods of the double-labeling kit, and belongs to the field of immunodetectionanalysis technologies and nano-biotechnologies. The kit comprises a reaction buffer solution integrated on a reagent strip, a cleaning solution, an enhancement solution, a magnetic particle solution coating a cTnI monoclonal antibody, a europium-labeled cTnC monoclonal antibody solution and a samarium-labeled cTnI monoclonal antibody solution and 2-6 bottles of additional freeze-dried calibrationproducts of natural cTnI antigens (containing cTnC) with different concentrations. The kit can provide a nearly-homogeneous double-labeling reaction system to ensure that the content of cTnI with complete fragments and the cTnI-C compound is detected simultaneously, the detection time is shortened, the efficiency is improved, and convenience is brought to clinical use.

Description

technical field [0001] The invention belongs to the field of immunoassay analysis technology and nano-biotechnology, in particular to a double-labeled kit for detecting troponin and its complex and a preparation and detection method. Background technique [0002] Cardiac troponin (cTn) is a regulatory protein of cardiac muscle contraction. cTn is composed of subunits of three different genes: cardiac troponin I (cTn I) and troponin C (TnC), and cardiac troponin T (cTnT). [0003] cTn exists in cardiomyocytes in the form of cTnI-C-T complex and free cTnI. After the myocardial injury is released into the blood circulation, cTnI-C-T can be further decomposed into cTnI-C complex and free cTnT, and cTnI-C is its The major form in the blood. Since their content in normal serum is very small, they are significantly increased in acute myocardial infarction (AMI). When cardiomyocytes are damaged by factors such as ischemia and hypoxia, free cTnI and cTnT can be rapidly released fr...

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Application Information

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IPC IPC(8): G01N33/68G01N33/577
CPCG01N33/577G01N33/68
Inventor 黄飚任志奇静亚茹
Owner 浙江博实生物科技有限公司
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