Compound microecological preparation and preparation method and application thereof

A technology of compound microecology and preparation, applied in the direction of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of inability to improve egg quality, high feed-to-egg ratio, poor safety, etc., and achieve high quality, high activity Bacterial count and the effect of improving production performance

Active Publication Date: 2018-03-23
TIANJIN BIOFEED TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In view of the above problems, the present invention provides a compound probiotic preparation and its preparation method and application

Method used

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  • Compound microecological preparation and preparation method and application thereof
  • Compound microecological preparation and preparation method and application thereof
  • Compound microecological preparation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Primary screening of strains

[0056] From the soil next to the auxiliary building of the Feed Research Institute of the Chinese Academy of Agricultural Sciences, lactic acid bacteria, bacillus and yeast were screened by natural selection method.

[0057] (1') Lactic acid bacteria screening

[0058] The collected samples were serially diluted with sterile water, and three gradients were selected to apply separation solid medium (tryptone 10g, yeast extract 10g, 12% skimmed milk powder emulsion 200mL, agar 15g, CaCO 3 3g, H 2 O 800mL, pH 6.5, sterilized at 115°C for 20min), cultured at 37°C for 48h, selected strains producing calcium soluble circles for colony observation, streaked and purified strains with different colony shapes, and observed the morphology under a microscope.

[0059] (2') Bacillus Screening

[0060] The collected samples were serially diluted with sterile water, and three gradients were selected for 80°C water bath treatment for 10 minutes, t...

Embodiment 2

[0065] Embodiment 2 compound probiotics (I)

[0066]Lactobacillus plantarum (Lactobacillus) BFC1602 (CGMCC No.13132), Bacillus subtilis (Bacillus subtilis) BFC1601 (CGMCC No.13131) and Saccharomyces cerevisiae (Saccharomycescerevisiae) BFC1603 (CGMCC No.13133) were fermented separately, centrifuged Fermentation broth and wet cells were collected, and the cells were resuspended with physiological saline. Mix Lactobacillus BFC1602, Bacillus subtilis BFC1601 and Saccharomyces cerevisiae BFC1603 in a ratio of 3:4:6 to prepare a microbial mixture. After the microbial mixture and the protective agent are mixed in a mass ratio of 3:4, the coating solution is added for coating. The mass ratio of the microbial mixture to the coating solution is 2:3.5. The formulations of the protective agent and the coating solution are shown in Table 1.

[0067] In the prepared compound microecological preparation, the number of live bacteria of plant Lactobacillus (Lactobacillus) BFC1602 was 5.0×10...

Embodiment 3

[0070] Embodiment 3 compound probiotics (II)

[0071] Lactobacillus plantarum (Lactobacillus) BFC1602 (CGMCC No.13132), Bacillus subtilis (Bacillus subtilis) BFC1601 (CGMCC No.13131) and Saccharomyces cerevisiae (Saccharomycescerevisiae) BFC1603 (CGMCC No.13133) were fermented separately, centrifuged Fermentation broth and wet cells were collected, and the cells were resuspended with phosphate buffer (pH 6.8). Mix Lactobacillus BFC1602, Bacillus subtilis BFC1601 and Saccharomyces cerevisiae BFC1603 in a ratio of 1:2:4 to prepare a microbial mixture. After the microbial mixture and the protective agent are mixed in a mass ratio of 1:3, the coating solution is added for coating. The mass ratio of the microbial mixture to the coating solution is 1:3. The formulations of the protective agent and the coating solution are shown in Table 2.

[0072] In the prepared compound microecological preparation, the number of live bacteria of plant Lactobacillus (Lactobacillus) BFC1602 was 4...

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Abstract

The invention provides a composite microecological preparation and a preparation method and application thereof. The composite microecological preparation is prepared from lactobacillus plantarum, bacillus subtilis and saccharomyces cerevisiae. The composite microecological preparation as a feed additive for laying hens has a higher viable cell count, has the advantages of improving the productionperformance of the laying hens, delaying the decline of an egg production rate, and improving the quality of eggs, and has broad application prospects and great market value.

Description

technical field [0001] The invention belongs to the field of microecological preparations, and relates to a composite microecological preparation and a preparation method and application thereof. Background technique [0002] Eggs are a great source of protein for humans. At present, laying hens are usually fed in cages. The eggs produced are large in size and high in yield, but the taste and nutritional value are often not as good as those produced by ordinary domestic laying hens, and caged laying hens also generally have poor immunity. Low gastrointestinal function and poor ovarian function. How to improve the immunity, gastrointestinal function and ovarian function of laying hens, improve the taste and nutritional value of eggs, and narrow the quality gap between caged eggs and domestic eggs has always been the primary problem that researchers need to solve. [0003] Adding microecological feed additives to the feed is an important way to adjust the intestinal microeco...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18A23K50/75A23K10/18A23K20/163A23K20/174A23K10/20A23K20/105A23K40/30C12R1/25C12R1/125C12R1/865
CPCA23K10/18A23K10/20A23K20/105A23K20/163A23K20/174A23K40/30A23K50/75C12N1/18C12N1/20
Inventor 邓雪娟蔡辉益王春雨刘世杰刘晶晶李宗平程善燕
Owner TIANJIN BIOFEED TECH CO LTD
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