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A kind of composite microbial agent for water environment treatment and its application

A technology of compound microbial inoculants and inoculants, applied in microorganism-based methods, microorganisms, water pollutants, etc., can solve the problems of nitrite and nitrate accumulation, poor adaptability, and low total nitrogen removal rate. The effect of eliminating black odor, strong ability and improving sediment condition

Active Publication Date: 2020-09-29
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the problems existing in the prior art, the object of the present invention is to provide a compound microbial bacterial agent with high efficiency and safety, which can improve the poor adaptability of traditional bacterial agents, the accumulation of nitrite and nitrate, and the low removal rate of total nitrogen. and other defects, providing effective solutions for my country's water environment governance

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  • A kind of composite microbial agent for water environment treatment and its application
  • A kind of composite microbial agent for water environment treatment and its application
  • A kind of composite microbial agent for water environment treatment and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The screening of each bacterial strain in the composite microbial bacterial agent of embodiment 1

[0054] 1. Avoid the compatibility of antagonistic strains

[0055] There are multiple survival relationships among microorganisms, including the possibility that strains of different species may antagonize each other, that is, the strains may inhibit or kill other functional strains by metabolizing certain substances and weaken the denitrification ability of the denitrification bacteria group. In order to ensure that the denitrification bacteria group can stably exert its denitrification effect, the compatibility of antagonistic strains should be avoided as much as possible. In this example, antagonistic tests were performed on all candidate strains with nitrogen removal function, and the strains with particularly significant antagonistic effects were eliminated. Further narrow the scope, and conduct antagonism test against the strains with better nitrogen removal functi...

Embodiment 2

[0073] The preparation of embodiment 2 composite microbial bacterial agents

[0074] 1) Solid slope culture: inoculate Pseudomonas otitidis, Pseudomonas kunmingensis, Pseudomonas asiatica and Alcaligenes sp. Cultivate in slant medium to fully activate the strain;

[0075] 2) Primary seed cultivation: inoculate the four activated bacteria in step 1) respectively in sterile liquid medium, and continuously shake and cultivate for 12-18 hours to obtain the primary seed cultivation liquid;

[0076] 3) Secondary seed culture: the obtained primary seed culture bacterial liquid is connected to the fermenter culture liquid with an inoculum amount of 10% volume ratio, and continuously shakes and cultivates for 10-12 hours;

[0077] 4) Inoculate the secondary seed liquids of the four microorganisms obtained in step 3) into the fermentation medium respectively, carry out high-density fermentation culture, and obtain bacterial agents A, B, C, and D respectively;

[0078] 5) Mix bacterial...

Embodiment 3

[0080] Embodiment 3 Composite microbial liquid bacterial agent of the present invention

[0081] The compound microbial bacterial agent is in the form of bacterial liquid, including

[0082] The viable count of Pseudomonas otitidis was 4×10 7 -6×10 8 cfu / mL, the viable count of Kunming Pseudomonas (Pseudomonas kunmingensis) is 4×10 7 -6×10 8 cfu / mL, the viable count of Pseudomonas asiatica is 1×10 8 -6×10 8 cfu / mL, the viable count of Alcaligenes sp. is 2×10 8 -2×10 9 cfu / mL.

[0083] Composite microbial liquid bacterial agent 1: composed of Pseudomonas otitidis, Pseudomonas kunmingensis, Pseudomonas asiatica, Alcaligenes sp. The effective viable counts of the aforementioned four microorganisms in Agent 1 were: 4×10 8 cfu / mL, 4×10 8 cfu / mL, 4×10 8 cfu / mL, 4×10 8 cfu / mL.

[0084] Bacteria 2: Composed of Pseudomonas otitidis, Pseudomonas kunmingensis, Pseudomonas asiatica, and Alcaligenes sp., as mentioned above in Bacteria 2 The effective viable counts of the four...

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Abstract

The invention relates to composite microbial agents, in particular to a composite microbial agent for water environment treatment and an application thereof. The composite microbial agent comprises: Pseudomonas otitidis, Pseudomonas kunmingensis, Pseudomonas asiatica, and Alcaligenes sp., and the total viable cell count of the microbial agent is not less than 1 x 10<9> cfu / mL. The composite microbial agent has low cost and no pollution to environment, a polluted water body is treated at an adding ratio of 0.01%-0.1%, after 1-2 weeks, removal efficiency of ammonia nitrogen is close to 100%, andtotal nitrogen elimination is up to 60% or more. At the same time, no accumulation of nitrites and nitrates is caused, the functional strain is traced in situ after repairing is completed, and thus relatively strong adaptability of the microbial agent in an actual water environment is verified. The composite microbial agent can be used for water environment treatment, and a feasible method for elimination of black odorous water body in China is provided.

Description

technical field [0001] The invention relates to the technical field of composite microbial bacterial agent and water environment treatment, in particular to a composite microbial bacterial agent for water environment treatment and its application. Background technique [0002] The black and odorous water body is mainly caused by the eutrophication of the water body. Excessive nutrients are conducive to the rapid reproduction of microorganisms, and consume dissolved oxygen in the water body and cause a large number of anaerobic microorganisms to multiply, thereby producing a large number of odors (hydrogen sulfide, ammonium etc.) and reduced (FeS, MnS, etc.) substances. [0003] At present, water environment treatment is mainly through interception and emission reduction, improvement of water body purification, dredging, restoration, etc. Among them, bioremediation technology, especially the addition of microbial agents, such as the use of nitrification and denitrification ba...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F11/02C02F101/16C12R1/38
CPCC02F3/34C02F11/02C02F2101/16C02F2101/163C02F2101/166C02F2103/007C02F2209/14C02F2209/15C02F2209/16C02F2303/02C12N1/20
Inventor 刘双江朱愿刘志培冯婕姜成英魏大伟彭潇柳晓东高喜燕刘缨吴梦茹
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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