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Clinical ready-to-use active preservation method for adipose-derived stem cells

A preservation method and stem cell technology, applied in the field of cell preservation media

Active Publication Date: 2020-04-14
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The clinical treatment process has its particularity. For example, if the patient feels uncomfortable after liposuction, the hospital equipment, equipment failure or other reasons cannot use cell products in time, then how to preserve the ADSCs during the period before treatment can maintain the best activity of ADSCs and facilitate The safe and effective use of cell products is a problem worth studying

Method used

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  • Clinical ready-to-use active preservation method for adipose-derived stem cells
  • Clinical ready-to-use active preservation method for adipose-derived stem cells

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Embodiment 1

[0036] A method for clinical ready-to-use active preservation of adipose-derived stem cells, comprising the following steps:

[0037] (1) Separation of adipose-derived stem cells and grouping of temperature and cell preservation solution:

[0038] ①Adipose tissue from 6 cases of 25-40-year-old female thigh and abdomen liposuction was taken, and adipose-derived stem cells were extracted by the same 0.1% collagenase digestion and centrifugation method, planted and subcultured at the same cell density, and P2 generation cells were obtained ;

[0039] ②Take well-growing P2 generation cells, wash each culture bottle with PBS, use 1mL 0.25% trypsin, place in a 37°C incubator to digest for 5min, add 3mL PBS buffer to dilute, centrifuge at 300g for 10min, discard the supernatant, and add 1mL PBS buffer, after blowing gently, the cell counting plate is counted and recorded;

[0040] ③Prepare 14 test tubes in the ultra-clean bench, each with 5×10 5 The cell density was suspended in 1...

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Abstract

The invention discloses a clinically ready-to-use vital preservation method for adipose-derived stem cells. The method comprises the following steps of suspending the adipose-derived stem cells into a non-immunogenicity human-derived serum or a human platelet rich plasma with the volume fraction being 10 + / -0.5 percent, and standing and preserving at 4 DEG C. The clinically ready-to-use vital preservation method for the adipose-derived stem cells provided by the invention is applicable to a ready-to-use ADSCs clinic treatment applying technique, and the ADSCs are provided with the optimal preservation temperature and the most suitable cell preservation medium during transportation or a short-time preservation process, so that the clinically ready-to-use ADSCs maintains the optimal cell activity before being applied in a human body, the effectiveness and the safety of the application of the clinical adipose-derived stem cells are improved, and clinical application and a clinical technology of the autologous adipose-derived stem cells are conveniently popularized. The preservation method provided by the invention has the advantages of conformation to theclinical standard, economy, simplicity, convenience, and capability of better maintaining cell activity. With popularization and application of the clinically ready-to-use vital preservation method for the adipose-derived stem cells, the effectiveness and the safety of the clinical application of cell products can be improved, and great economic and social benefits can be achieved.

Description

technical field [0001] The invention relates to the best storage temperature and the most suitable cell storage medium during short-term transportation and storage, in particular to a clinical ready-to-use active storage method for adipose-derived stem cells. Background technique [0002] The short-term storage method of adipose-derived stem cells in clinical application is related to the effect and safety of cell therapy. The period from the preparation of the cell product to the time before the treatment (including the transportation of the cell product) keeps the cell product still in the most active state. In the best state or after entering the human body, it can exert a better therapeutic effect. Relevant literature reports on cell in vitro preservation techniques mainly include non-cryopreservation at 4°C, program-controlled cooling in liquid nitrogen (196°C), and direct cryopreservation at -80°C. The method of cryopreservation of cells mainly focuses on the long-ter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 刘宏伟廖选李朦李升红
Owner JINAN UNIVERSITY
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