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Method for improving infertility by employing stem cells

A technique for infertility and infertility, applied in the field of cell therapy, can solve the problems that mature eggs cannot be ingested, the operation of fertilized eggs is affected, and the implantation of fertilized eggs is adversely affected. Epithelial repair and improvement of premature ovarian failure

Pending Publication Date: 2020-05-19
卡替(上海)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2. Endometriosis Pelvic endometriosis and ovarian endometriosis can form peritoneal adhesions, which can cause adhesions outside the umbrella end of the fallopian tube or adhesions around the ovary, so that mature eggs cannot be taken into the fallopian tube; cause Extensive adhesions can also affect the operation of fertilized eggs
2. Abortion and curettage cause damage to the endometrium: the endometrium is divided into three layers, the top layer peels off every month, the middle layer and the bottom layer do not peel off, if the middle layer is damaged, the impact is not great, if the bottom layer Injured, then the consequences will be more serious
Abnormal thickening of the endometrium can lead to infertility. The endometrium is affected by hormone levels in the body. If excessive thickening is mainly caused by changes in hormone levels, it will not only affect menstruation, but may also affect normal menstruation. ovulation, and it is not conducive to the implantation of fertilized eggs, leading to habitual miscarriage or infertility

Method used

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Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with specific embodiments.

[0019] The invention discloses a method for improving infertility by using stem cells. The method specifically includes the following steps: firstly extract the patient's autologous adipose stem cells in a hospital, and the doctor needs to extract the patient's autologous adipose stem cells; Transport Autologous adipose stem cells are transported to the cell preparation center in a refrigerated state, and then the autologous adipose stem cells are cultured in a culture bottle in a GMP laboratory for passage and expansion; after 29 days to 35 days of culture, the amplified autologous adipose Stem cells are transported back to the hospital through cold chain transportation, and the expanded autologous adipose stem cells are reinfused into the body of infertile patients. The reinfusion method is intravenous or intramuscular injection. 20,000 to 60,000 units / kg, injected once...

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Abstract

The invention discloses a method for improving infertility by employing stem cells. Firstly, autologous adipose-derived stem cells of a patient are extracted in a hospital; then, the autologous adipose-derived stem cells are transported under cold storage to a cell preparation center by cold-chain transportation; then, the autologous adipose-derived stem cells are cultured in a culture flask for passaging and proliferation in a good manufacturing practice (GMP) lab; after the autologous adipose-derived stem cells are cultured for 29 days to 35 days, the proliferated autologous adipose-derivedstem cells are transported back to the hospital in a manner of cold-chain transportation; the proliferated autologous adipose-derived stem cells are injected back into the body of the infertility patient; and the number of the autologous adipose-derived stem cells injected each time is 20000-60000 units per kg, the autologous adipose-derived stem cells are injected once at intervals of 8-10 days,every four times of injection is taken as a course of treatment, and the autologous adipose-derived stem cells need to be continuously injected for 10-20 courses of treatment in total. The method disclosed by the invention can bring new hope for infertility patients; the stem cells can repair the endometrium of an infertility woman to make the endometrium regenerated, and premature ovarian failureis effectively improved, thus being conducive to androlepsis.

Description

technical field [0001] The invention belongs to the technical field of cell therapy, and in particular relates to a method for improving infertility by using stem cells. Background technique [0002] The medical definition of infertility is that no contraceptive measures have been taken for more than one year, and the sex life is normal without successful pregnancy. It is mainly divided into primary infertility and secondary infertility. Primary infertility is never conceived, and secondary infertility is infertility after being pregnant. By this strict definition, infertility is a common problem, affecting at least 10% to 15% of couples of reproductive age. According to the prediction of WHO, infertility in the 21st century will become the third major disease next to tumors and cardiovascular and cerebrovascular diseases. It is an important symbol to reflect the happiness of the family and the quality of life, and it is also an important indicator to measure the actual si...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P15/08
CPCA61K9/0019A61K35/28A61P15/08
Inventor 李军
Owner 卡替(上海)生物技术有限公司
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