A method for specific stem cells to repair diseased pancreatic islets

A technology of stem cells and islets, applied in the field of stem cells, can solve the problems affecting the survival and expansion of cells on the scaffold, large physical size, etc., and achieve the effect of promoting recovery as soon as possible, increasing the success rate, and speeding up the speed

Active Publication Date: 2021-09-03
朗姿赛尔生物科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the traditional porous scaffold has a large physical size, which hinders the entry of nutrients and cells into the scaffold, seriously affecting the survival and expansion of cells on the scaffold.

Method used

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  • A method for specific stem cells to repair diseased pancreatic islets

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for repairing diseased islets with specific stem cells, comprising:

[0030] S11. The cells obtained after centrifugation of autologous adipose-derived mesenchymal cells are cultured with adipose-derived stem cells and passed to P3 generation;

[0031] S12. Digest the P3 generation adipose-derived mesenchymal stem cells and resuspend them in the adipose-derived mesenchymal stem cell medium, culture for 36 hours, then replace the medium in half every 72 hours, cultivate for 8 days, and obtain the cell fluid, which includes Islet acellular matrix;

[0032] S13. Add nanoporous micro-scaffolds to the PBS solution, disperse and mix with the cell solution to obtain the islet repair solution, and inject the islet repair solution to the islets. The preparation method of the islet decellularized matrix is:

[0033] S21. Take out the mouse islet tissue under aseptic conditions, rinse with PBS until the cleaning solution is clear, and then immerse in PBS to repeatedly f...

Embodiment 2

[0041] A method for repairing diseased islets with specific stem cells, comprising:

[0042] S11. The cells obtained after centrifugation of autologous adipose-derived mesenchymal cells are cultured with adipose-derived stem cells and passed to P3 generation;

[0043] S12. Digest the P3 generation adipose-derived mesenchymal stem cells and resuspend them in the adipose-derived mesenchymal stem cell medium, culture for 36 hours, then replace the medium in half every 72 hours, cultivate for 8 days, and obtain the cell fluid, which includes Islet acellular matrix;

[0044] S13. Add nanoporous micro-scaffolds to the PBS solution, disperse and mix with the cell solution to obtain the islet repair solution, and inject the islet repair solution to the islets. The preparation method of the islet decellularized matrix is:

[0045] S21. Take out the mouse islet tissue under aseptic conditions, wash it with PBS until the cleaning solution is clear, and then immerse it in PBS to repeate...

Embodiment 3

[0051] A method for repairing diseased islets with specific stem cells, comprising:

[0052] S11. The cells obtained after centrifugation of autologous adipose-derived mesenchymal cells are cultured with adipose-derived stem cells and passed to P3 generation;

[0053] S12. Digest the P3 generation adipose-derived mesenchymal stem cells and resuspend them in the adipose-derived mesenchymal stem cell medium, culture for 36 hours, then replace the medium in half every 72 hours, cultivate for 8 days, and obtain the cell fluid, which includes Islet acellular matrix;

[0054] S13. Add nanoporous micro-scaffolds to the PBS solution, disperse and mix with the cell solution to obtain the islet repair solution, and inject the islet repair solution to the islets. The preparation method of the islet decellularized matrix is:

[0055] S21. Take out the mouse islet tissue under aseptic conditions, rinse with PBS until the cleaning solution is clear, then immerse in PBS to repeatedly freez...

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Abstract

The invention relates to the field of stem cells, and provides a method for repairing diseased pancreatic islets by specific stem cells, which is used to improve the repairing effect of pancreatic islets. A method for repairing diseased islets with specific stem cells provided by the present invention, comprising: S11. The cells obtained after centrifuging autologous adipose-derived mesenchymal cells are cultured with adipose-derived stem cells and passed to the P3 generation; S12. The P3-generation adipose-derived mesenchymal Stem cells were digested and resuspended in adipose-derived mesenchymal stem cell culture medium, cultured for 36 hours, then half of the culture medium was replaced every 72 hours, cultured for 8 days, and cell fluid was obtained, and the medium contained pancreatic islet decellularized matrix; S13. In PBS The nanoporous micro-stent is added into the solution, dispersed and mixed with the cell solution to obtain the islet repair solution, and the islet repair solution is injected into the islet. The specific stem cells selected from the autologous body can improve the success rate of islet repair and promote the timely transformation of diseased islets into normal tissues.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a method for repairing diseased pancreatic islets by specific stem cells. Background technique [0002] Adipose-derived mesenchymal stem cells are cells with multi-directional differentiation potential, which can be directed to differentiate into islet-like cells, chondrocytes and other cells. Adipose-derived mesenchymal stem cells have the advantages of wide sources, convenient materials, little damage to the donor site, and no ethical issues. It has been proved in the literature that adipose-derived mesenchymal stem cells can be induced to differentiate into pancreatic islet cells with insulin secretion function, which is a promising candidate for clinical treatment. Diabetes offers new technical solutions. However, in the method of directly injecting and transplanting the differentiated insulin-secreting cells into the body, these insulin-secreting cells will circulate with the bloo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775A61K35/39A61K47/02A61P5/50
CPCA61K9/0019A61K35/39A61K47/02A61K47/61A61P5/50C12N5/0667
Inventor 陈忠平
Owner 朗姿赛尔生物科技(广州)有限公司
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