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Method for producing composition for regenerating cartilage using freeze-dried hyaline cartilage powder, composition for regenerating cartilage produced using same, method for producing scaffold for regenerating patient-specific cartilage using composition for regenerating cartilage, and scaffold for regenerating patient-specific cartilage

A hyaline cartilage, patient-customized technology, used in the production of a cartilage regeneration composition using freeze-dried hyaline cartilage powder, the use of the produced cartilage regeneration composition, and the use of the cartilage regeneration composition to produce patient-customized cartilage regeneration scaffolds and In the field of cartilage regeneration scaffolds customized for patients, it can solve the problems of easy destruction of cartilage and difficult distribution and transplantation of cells, so as to achieve the effect of promoting cartilage regeneration.

Inactive Publication Date: 2021-11-26
ROKIT HEALTHCARE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to gravity, it is difficult for transplanted cells to be evenly distributed and transplanted to the damaged area, and there is a problem of inducing differentiation into fibrocartilage instead of hyaline cartilage, resulting in the phenomenon that cartilage is easily destroyed

Method used

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  • Method for producing composition for regenerating cartilage using freeze-dried hyaline cartilage powder, composition for regenerating cartilage produced using same, method for producing scaffold for regenerating patient-specific cartilage using composition for regenerating cartilage, and scaffold for regenerating patient-specific cartilage
  • Method for producing composition for regenerating cartilage using freeze-dried hyaline cartilage powder, composition for regenerating cartilage produced using same, method for producing scaffold for regenerating patient-specific cartilage using composition for regenerating cartilage, and scaffold for regenerating patient-specific cartilage
  • Method for producing composition for regenerating cartilage using freeze-dried hyaline cartilage powder, composition for regenerating cartilage produced using same, method for producing scaffold for regenerating patient-specific cartilage using composition for regenerating cartilage, and scaffold for regenerating patient-specific cartilage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] [Example 1] - the preparation of freeze-dried hyaline cartilage powder

[0090] After the obtained hyaline cartilage tissue was sliced, it was freeze-dried at a pressure of about 5 bar and a temperature of about -80° C. for about 12 hours in a near-vacuum atmosphere, and pulverized using a ball mill.

[0091] figure 2 is a set of images showing the freeze-dried hyaline cartilage tissue and its pulverization results according to Example 1. refer to figure 2 , different from figure 1 , it can be confirmed that hyaline cartilage powder is micronized into very fine particles without agglomeration.

[0092] That is, there may be problems in that the hyaline cartilage powder prepared as in Comparative Example 1 requires a separate dispersion process due to agglomeration and may clog a printer nozzle. In addition, the hyaline cartilage powder prepared as in Comparative Example 1 needs to be frozen at about -20°C, which is inconvenient. On the other hand, the freeze-drie...

experiment example 1

[0094] [Experimental Example 1]——Biocompatibility experiment of freeze-dried hyaline cartilage powder

[0095] To confirm the biocompatibility and regenerative capacity of the hyaline cartilage powder prepared as in Example 1, it was observed by hematoxylin and eosin (H&E) staining after injection into the dermis of laboratory mice, 4 weeks and 12 weeks later. Cytocompatibility results of freeze-dried hyaline cartilage powder.

[0096] Figure 4 The results after 4 weeks and 12 weeks according to Experimental Example 1 are shown. Specifically, refer to Figure 4 , After 4 weeks, it could be confirmed that the cells were concentrated around the implanted freeze-dried hyaline cartilage powder, and after 12 weeks, the cells were concentrated around the new tissue formed by the freeze-dried hyaline cartilage powder. Therefore, it could be confirmed that although the hyaline cartilage was freeze-dried, there were no side effects such as inflammation or reduction in tissue regene...

experiment example 2

[0097] [Experimental Example 2] - Animal experiment for confirmation of cartilage regeneration (in vivo experiment)

[0098] Autologous adipose tissue is obtained as follows: 70 to 150 mL of aspirate is taken from the abdomen of a laboratory beagle dog using liposuction under anesthesia, the aspirate is allowed to stand for about 5 minutes, and then the saline and the adipose tissue extracted with it are removed. sediment of blood.

[0099] Subsequently, the syringe containing the subject's fat and the fresh syringe were installed in the two-way inlet of the connector with a stainless steel syringe filter (Adnizer, SKT-AN-2400, BSL) with a pore size of 2.4 mm, and the fat was removed by piston movement. The fibers are removed from the autologous adipose tissue by passing it through the filter two or three times.

[0100] Subsequently, the fiber-free fat was injected into a filter bag (adipose tissue processing device, Tiss'you) containing a PET filter with a pore size of 500 ...

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Abstract

The present invention relates to a method for using a freeze-dried hyaline cartilage powder to produce a composition for regenerating cartilage, and a composition for regenerating cartilage produced by using the method, the method comprising: A) a step for preparing hyaline cartilage; B) a step for freeze-drying and crushing the hyaline cartilage, and producing a freeze-dried hyaline cartilage powder; C) a step for producing an adipose tissue extract from autologous adipose tissue; and D) a step for producing a composition which is for regenerating cartilage and including the freeze-dried hyaline cartilage powder and the adipose tissue extract.

Description

technical field [0001] This application claims priority and benefit from Korean Patent Application No. 10-2019-0027705 filed on March 11, 2019, the disclosure of which is incorporated herein by reference in its entirety. [0002] This specification relates to a method for preparing a composition for cartilage regeneration using freeze-dried hyaline cartilage powder, a composition for cartilage regeneration prepared by the method, a method for producing a patient-customized scaffold for cartilage regeneration using the composition for cartilage regeneration, and patient customization Scaffolds for cartilage regeneration. Background technique [0003] In the hinge part where human bones meet, articular cartilage composed of hyaline cartilage contacts each other and generates internal pressure and tension, and there is a synovial sac containing synovial fluid in each joint to reduce friction caused by joint movement. [0004] Although the cause of degenerative changes in artic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61F2/30
CPCA61L2400/06A61L2430/06B33Y80/00A61L27/3612A61L27/3604A61L27/3654A61L27/3691A61L27/50A61L27/222A61L27/225A61L27/16A61L27/18A61F2/30756A61F2/30942A61F2002/30766A61F2002/30957A61F2002/30985C08L35/04C08L75/04A61L27/3625B33Y70/10
Inventor 柳石焕
Owner ROKIT HEALTHCARE INC
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