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Angiotensin converting enzyme inhibitory peptide production method

A technique for angiotensin, inhibitory activity

Inactive Publication Date: 2006-12-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These indicate that there is a complex degradation mechanism in the enzymatic hydrolysis process

Method used

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  • Angiotensin converting enzyme inhibitory peptide production method

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1, a kind of production method of angiotensin-converting enzyme inhibitory active peptide, carries out following steps successively:

[0037] 1), after mixing rice grains and distilled water in the container according to the weight ratio of 1:4.13, adjust the pH value to 8.02 with a sodium hydroxide solution with a concentration of 2.5M;

[0038] 2), after adding trypsin into the container, put it into a constant temperature water bath for enzymolysis reaction; the temperature of the water bath is 37°C, the reaction time is 4 hours, and the amount of trypsin is 1.5% of the weight of rice grains;

[0039] 3), and then put the container into boiling water and boil for 10 minutes to terminate the hydrolysis reaction; the obtained product is centrifuged, and the supernatant obtained by centrifugation is concentrated, dried under vacuum freezing (vacuum degree 40-44Pa) at -45°C-30°C, The angiotensin-converting enzyme inhibitory active peptide is obtained.

[0040...

Embodiment 2

[0041] Embodiment 2, a kind of production method of angiotensin-converting enzyme inhibitory active peptide, carry out the following steps successively:

[0042] 1), after mixing rice grains and distilled water in the container according to the weight ratio of 1:4, adjust the pH value to 7.9 with a sodium hydroxide solution with a concentration of 2.0M;

[0043] 2), after adding trypsin into the container, put it into a constant temperature water bath for enzymolysis reaction; the temperature of the water bath is 35°C, the reaction time is 4.5 hours, and the amount of trypsin is 1.6% of the weight of rice grains;

[0044] 3), then put the container into boiling water and boil for 12 minutes to terminate the hydrolysis reaction; the obtained product is centrifuged, and the supernatant obtained by centrifugation is concentrated, dried under vacuum freezing (vacuum degree 40-44Pa) at -45°C-30°C, The angiotensin-converting enzyme inhibitory active peptide is obtained.

[0045] Th...

Embodiment 3

[0046] Embodiment 3, a kind of production method of angiotensin-converting enzyme inhibitory active peptide, carry out the following steps sequentially:

[0047] 1), after mixing rice grains and distilled water in the container according to the weight ratio of 1:4.5, adjust the pH value to 8.1 with a sodium hydroxide solution with a concentration of 3.0M;

[0048] 2), after adding trypsin into the container, put it into a constant temperature water bath to carry out the enzymolysis reaction; the temperature of the water bath is 40°C, the reaction time is 3.5 hours, and the amount of trypsin is 1.4% of the weight of rice grains;

[0049] 3), and then put the container into boiling water and boil for 8 minutes to terminate the hydrolysis reaction; the obtained product is centrifuged, and the supernatant obtained by centrifugation is concentrated, dried under vacuum freezing (vacuum degree 40-44Pa) at -45°C-30°C, The angiotensin-converting enzyme inhibitory active peptide is obta...

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Abstract

This invention exposed the production method of angiotensin-converting enzyme inhibitory petides. The steps are as follow: 1) mix the rice dregs and distiller in container by the proportion of 1:4 - 4.5, then adjust the pH value to 7.9 - 8.1 with alkali solutions of 2.0 - 3.0M density; 2) add trypsin in container then put it in constant temperature water bath to react; the temperature of water bath is 35 - 40DEG C, the time of reaction is 3.5 - 4.5 hours. The amount of trypsin is as much as 1.4 - 1.6% of the weight of rice dregs; 3) then put the container in boiling water to stop the reaction; separating the obtained product by centrifugal, then condensing, freezing and drying the supernatant, what obtained is angiotensin-converting enzyme inhibitory petides. This finally obtained angiotensin-converting enzyme inhibitory petides is safe, economy and effective.

Description

technical field [0001] The invention relates to a production method of an angiotensin-converting enzyme inhibitory active peptide. Background technique [0002] Rice is one of the main grains in the world. More than half of the world and more than two-thirds of the population in my country take rice as a staple food. my country's planting area ranks second in the world, and its total output ranks first in the world. The annual output is 185 million tons, accounting for about 36% of the world's total rice output. Most of these rices are used for industrial purposes, such as monosodium glutamate fermentation, starch sugar industry, etc., except for a part of supplying people's daily diet. A large number of by-products are produced in industrial production using rice as raw material, for example, a large amount of rice grains are produced in monosodium glutamate factories, glucose factories and other fermentation factories. Rice grains are produced after saccharification of r...

Claims

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Application Information

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IPC IPC(8): C12P21/02A61P9/12
Inventor 何国庆陈启和阮晖玄国东
Owner ZHEJIANG UNIV
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