Lactobacillus rhamnosus and application thereof in preparing bacteriocin
A technology of Lactobacillus rhamnosus and bacteriocin, which is applied in the field of food microorganisms, can solve the problems of drug resistance of pathogenic bacteria and threaten human health, and achieve the effects of safe source, less toxic and side effects, and good thermal stability
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[0019] The Lactobacillus rhamnosus zrx01 was isolated and screened from kefir grains. The frozen zrx01 strain was inoculated into MRS medium and cultured at a constant temperature of 200r / min at 37°C for 24h as the first-level seed solution. The primary seeds were inoculated into 100 mL MRS medium at an inoculum amount of 2%, cultivated overnight as secondary seed liquid, and kept in a refrigerator at 4°C. Streak the cultured secondary seed solution zrx01 plate on the MRS plate and incubate at 37°C for 2-3 days to observe the colony morphology. A single colony was picked and Gram stained, and the morphology of the bacteria was observed under a microscope. The results showed that the colony of strain zrx01 was in the form of moist milky white droplets, opaque, uniform in texture, and neat edges. After staining with Gram stain and microscopic observation, the bacteria became short rod-shaped Gram-positive bacteria ( figure 1 ). The biochemical characteristics (Table 1) show tha...
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