Lactobacillus rhamnosus and application thereof in preparing bacteriocin

A technology of Lactobacillus rhamnosus and bacteriocin, which is applied in the field of food microorganisms, can solve the problems of drug resistance of pathogenic bacteria and threaten human health, and achieve the effects of safe source, less toxic and side effects, and good thermal stability

Active Publication Date: 2018-06-12
HENAN INST OF SCI & TECH
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  • Description
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  • Application Information

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Problems solved by technology

The growth of pathogenic bacteria can be inhibited by chemical drugs, but the abuse of antibiotics makes pathogeni

Method used

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  • Lactobacillus rhamnosus and application thereof in preparing bacteriocin
  • Lactobacillus rhamnosus and application thereof in preparing bacteriocin
  • Lactobacillus rhamnosus and application thereof in preparing bacteriocin

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Embodiment 1

[0019] The Lactobacillus rhamnosus zrx01 was isolated and screened from kefir grains. The frozen zrx01 strain was inoculated into MRS medium and cultured at a constant temperature of 200r / min at 37°C for 24h as the first-level seed solution. The primary seeds were inoculated into 100 mL MRS medium at an inoculum amount of 2%, cultivated overnight as secondary seed liquid, and kept in a refrigerator at 4°C. Streak the cultured secondary seed solution zrx01 plate on the MRS plate and incubate at 37°C for 2-3 days to observe the colony morphology. A single colony was picked and Gram stained, and the morphology of the bacteria was observed under a microscope. The results showed that the colony of strain zrx01 was in the form of moist milky white droplets, opaque, uniform in texture, and neat edges. After staining with Gram stain and microscopic observation, the bacteria became short rod-shaped Gram-positive bacteria ( figure 1 ). The biochemical characteristics (Table 1) show tha...

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Abstract

The invention discloses lactobacillus rhamnosus and application thereof in preparing bacteriocin. The preservation taxonomic name of lactobacillus rhamnosus is lactobacillus rhamnosus zrx01, the lactobacillus rhamnosus is preserved in the China General Microbiological Culture Collection Center on December 22nd, 2017, and the preservation number of lactobacillus rhamnosus is CGMCC No.15076. The bacteriocin generated by the screened lactobacillus rhamnosus zrx01 can inhibit various harmful bacteria, especially has a strong inhibition function on gram-positive staphylococcus aureus, Listeria monocytogenes, bacillus anthraci, gram-negative escherichia coli, campylobacter jejuni and acid soil alicyclobacillus spp, has no inhibition function on lactobacillus plantarum, lactobacillus acidophilusand other probiotics, and also has no inhibition function on lactobacillus rhamnosus zrx01 and most bacteriocin.

Description

Technical field [0001] The invention belongs to the technical field of food microorganisms, and specifically relates to a Lactobacillus rhamnosus and its application in preparing bacteriocins. Background technique [0002] Food safety is an important aspect of people's choice of food. Among them, diseases caused by food-borne microorganisms are widespread in both developed and developing countries, seriously threatening public health. After years of research, food pathogenic microorganisms related to health hazards have been identified, such as Salmonella enteritidis and Eschericia coli, which cause 75% of foodborne diseases. Chemical drugs can inhibit the growth of pathogenic bacteria. However, the abuse of antibiotics makes pathogens resistant to disease and seriously threatens human health. Therefore, natural products have received more and more attention. Bacteriocins are peptides or protein substances with antibacterial activity produced by lactic acid bacteria. They have a...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/00C12R1/225
CPCC07K14/335C12N1/205C12R2001/225
Inventor 冉军舰朱明明焦凌霞梁新红赵瑞香卢艳清
Owner HENAN INST OF SCI & TECH
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