The invention discloses a dendrobium candidum tissue culture seedling culture medium for increasing alkaloid and a preparing method thereof. The dendrobium candidum tissue culture seedling culture medium is prepared from apple juice, citric acid, saccharin soluble hydrate, tea polyphenol, sylvite, microcosmic salt, nitrogen salt, calcium salt, magnesium salt, iodate, boron salt, molybdenum salt, zinc salt, manganese salt, copper salt, ferric salt, vitamin, amino acid, indolebutyric acid, gibberellin, 6-benzylaminopurine, naphthylacetic acid, kinetin, asparagine, conditioner, cosolvent, a dispersing agent, compatilizer, stabilizer, an antibacterial agent and water. According to the dendrobium candidum tissue culture seedling culture medium, the obtained seedling is good in quality and high in survival rate, and can effectively improve the content of alkaloid, polysaccharide and amino acid.