The present invention discloses a 3Cr2Mo die steel heat treatment process which is characterized by comprising the following steps, to be more specific, (1) forging; (2) annealing, to be more specific, heating to the temperature of 850 DEG C, performing thermal insulation for 2-4h, at isothermal temperature of 720 DEG C, performing thermal insulation for 4-6h, then cooling in a furnace to 500 DEG C, and taking out of the furnace to cool in the air; (3) quenching and tempering, to be more specific, at the quenching heating temperature of 860-870 DEG C, performing oil quenching, and at 540-580 DEG C performing tempering, wherein the surface hardness is 30-35HRC; and (4) carburizing process, to be more specific, heating at 900 DEG C for (6-8) h, after infiltration, at 850-870 DEG C performing oil quenching, and then performing low temperature tempering at 200-250 DEG C for (2-3) h. The 3Cr2Mo die steel heat treatment process can improve organizational stability of 3Cr2Mo die steel, and has high hot hardness, wear resistance and toughness and other overall performances.