The invention discloses a heat-resistant aluminum alloy containing Mo-Be and a preparation method of the heat-resistant aluminum alloy containing Mo-Be and belongs to the field of metal material engineering. The heat-resistant aluminum alloy containing Mo-Be is prepared from, by mass percent, 6%-7% of Cu, 0.5%-0.7% of Mn, 0.5%-0.7% of Ni, 0.3%-0.4% of Cr, 0.3%-0.4% of Be, 0.3%-0.4% of Mo, 0.2%-0.3% of V, 0.15%-0.25% of Ti, 0.01%-0.015% of B, 2.5%-3% of Ce, 1.5%-2% of La and the balance Al and inevitable impurities. The preparation method includes the steps that firstly, an aluminum and chromium intermediate alloy is molten, after other intermediate alloys and aluminum are added, a covering agent is added, slag is pushed aside after heat preservation, an aluminum refining agent is added, and slag is separated after heat heat preservation; secondly, casting is performed; and thirdly, cast blanks are subjected to solid solution and aging treatment. According to the alloy, due to multi-element alloying, the component range of the alloy is adjusted, high-temperature strength and high-temperature lasting performance of the alloy are remarkably improved after heat treatment, and the highest transient tensile strength reaches 260 MPa-270 MPa at 250 DEG C. Tensile strength ranges from 233 Mpa to 238 Mpa after heating is performed at 200 DEG C for 200 h, and tensile strength ranges from 187 Mpa to 195 Mpa after heating is performed at 250 DEG C for 200 h.