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A magnetically responsive multi-gradient drug-loaded microsphere prepared by double in situ hybridization

A multi-gradient, magnetic responsive technology, applied in the fields of organic active ingredients, pharmaceutical science, drug combination, etc., to achieve the effect of simple preparation procedure, good biomagnetic orientation, and easy operation

Active Publication Date: 2017-04-12
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the combination of traditional Chinese medicine and biomaterials in the field of bone tissue repair at home and abroad is still in its preliminary stage

Method used

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  • A magnetically responsive multi-gradient drug-loaded microsphere prepared by double in situ hybridization
  • A magnetically responsive multi-gradient drug-loaded microsphere prepared by double in situ hybridization
  • A magnetically responsive multi-gradient drug-loaded microsphere prepared by double in situ hybridization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Dissolving icariin in absolute ethanol, dissolving chitosan in an acetic acid solution with a volume fraction of 1%, and adding the alcohol solution of icariin to the acetic acid solution of chitosan under magnetic stirring , stirring to make each component mix evenly, and the content of chitosan and icariin calculated by mass percentage is 2% and 0.05% respectively;

[0037] (2) According to n(Fe 3+ ): n(Fe 2+)=2:1 ratio, a certain amount of soluble iron salt and soluble ferrous salt were added to the above mixture, and the two were continuously stirred for 30min, and the theoretically formed Fe 3 o 4 The mass ratio is 2%;

[0038] (3) Prepare a 1.5mol / L soluble calcium salt solution, add it to the mixed solution obtained in (2), and keep stirring to make it fully mixed evenly to obtain a mixed solution of microsphere precursor;

[0039] (4) Prepare 0.5mol / L NaOH ethanol / water solution, wherein the volume ratio of ethanol to water is 1~2:1, and prepare 1.8mol / L...

Embodiment 2

[0044] (1) Dissolving icariin in absolute ethanol, dissolving chitosan in an acetic acid solution with a volume fraction of 1%, and adding the alcohol solution of icariin to the acetic acid solution of chitosan under magnetic stirring , stirring to make each component mix evenly, and the contents of chitosan and icariin calculated by mass percentage are 1.5%, 0.08% respectively;

[0045] (2) According to n(Fe 3+ ): n(Fe 2+ )=2:1 ratio, a certain amount of soluble iron salt and soluble ferrous salt were added to the above mixture, and the two were continuously stirred for 45min, and the theoretically formed Fe 3 o 4 The mass ratio is 3%;

[0046] (3) Prepare 2.4mol / L soluble calcium salt solution, add it to the mixed solution obtained in (2), and keep stirring to make it fully mixed evenly to obtain the microsphere precursor mixed solution;

[0047] (4) Prepare 1.0mol / L NaOH ethanol / water solution, wherein the volume ratio of ethanol to water is 1~2:1, and prepare 1.2mol / L ...

Embodiment 3

[0052] (1) Dissolving icariin in absolute ethanol, dissolving chitosan in an acetic acid solution with a volume fraction of 2%, and under magnetic stirring, add the alcohol solution of icariin to the acetic acid solution of chitosan , stirring to make each component mix evenly, and the content of chitosan and icariin calculated by mass percentage is 2.5%, 0.03% respectively;

[0053] (2) According to n(Fe 3+ ): n(Fe 2+ )=2:1 ratio, a certain amount of soluble ferrous salt and soluble ferrous salt were added to the above mixture, and the two were continuously stirred for 1h, and the theoretically formed Fe 3 o 4 The mass ratio is 4%;

[0054] (3) Prepare a 3mol / L soluble calcium salt solution, add it to the mixed solution obtained in (2), and keep stirring to make it fully mixed evenly to obtain the microsphere precursor mixed solution;

[0055] (4) Prepare 1.5mol / L NaOH ethanol / water solution, wherein the volume ratio of ethanol to water is 1-2:1, and prepare 0.6mol / L solu...

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Abstract

The invention discloses a multi-gradient drug-loading microsphere having magnetic response and prepared through dual in-situ hybridization. According to the dual in-situ hybridization principle and the nano self-assembly technology, a bone repair drug-loading microsphere is developed; chitosan serves as an organic matrix, ferric chloride and ferrite serve as precursors of inorganic phase Fe3O4, and a calcium-microcosmic salt solution serves as a precursor of inorganic phase hydroxyapatite; a chitosan solution containing traditional Chinese medicine and icariin is fully and evenly mixed with the ferric chloride and the ferrite firstly, and then is evenly and fully mixed with soluble calcium salt, by means of a high-voltage static microsphere forming device, the mixture is dipped into an alkali liquor containing soluble phosphate drop by drop, the mode that organic macromolecules are adopted in biomineralization to control inorganic phase growth is simulated, the organic macromolecules become nuclear crystals on the organic matrix in a dual in-situ hybridization mode, and the multi-gradient drug-loading microsphere is prepared. The novel bone repair material is simple in preparation process, biocompatibility is good, and the capacity of controlling slow release of medicine is obviously enhanced through unique biological magnetic guide quality and multi-gradient performance.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and specifically relates to a multi-gradient drug-loaded composite microsphere and a preparation method thereof developed according to the principle of double in-situ hybridization and nanometer self-assembly technology. Background technique [0002] The repair of bone defects caused by tumors, tuberculosis, trauma and metabolic bone diseases has always been one of the difficult problems in the field of biomedicine. Currently commonly used allograft bone transplantation, autologous bone transplantation and other methods have some problems in varying degrees. For example, autologous bone sources are extremely limited and cannot be used for large bone defects; allogeneic bone has immune rejection and may cause pathogen infection. In recent years, with the development of biomedical engineering, drug delivery system has attracted more and more attention as an excellent bone repair mater...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/04A61L27/46A61L27/54A61L27/50A61K9/16A61K31/7048A61K41/00A61K47/36A61P19/08
Inventor 陈景帝潘盼盼张玉珏钟胜男张其清
Owner FUZHOU UNIV
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