Nanometer vauqueline liposome and preparation method thereof

A technology of strychnine liposome and strychnine, which is applied in the field of nano strychnine liposome and its preparation, can solve the problems of experimental research and clinical application limitations, high toxicity, etc.

Inactive Publication Date: 2010-12-08
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its high toxicity, experimental research and clinical application are greatly limited.
There is no clinical report on the direct application of strychnine

Method used

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  • Nanometer vauqueline liposome and preparation method thereof
  • Nanometer vauqueline liposome and preparation method thereof
  • Nanometer vauqueline liposome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Weigh 60mg of strychnine, 450mg of hydrogenated lecithin and 150mg of protein cholesterol and dissolve them in 20ml of chloroform, transfer them to a round bottom flask, and evaporate the organic solvent under reduced pressure at a temperature of 40°C at a speed of 150r / min. The film was dried under vacuum at room temperature for 24 hours. Then add 20ml of Tween 80 aqueous solution with a concentration of 9mg / ml and 40ml of phosphate buffer, adjust the pH to 7.2, fully swell for half an hour, and then ultrasonicate in a water bath for 10min, and then use an ultrasonic cell pulverizer at a certain power Ultrasonic for 20 minutes, the strychnine liposome with white opalescence was obtained. Electron microscope photos such as figure 1 , The liposome particle size is about 200nm.

[0032] Ultrasonic cell pulverizer is a general-purpose equipment, and the model JYD-900 ultrasonic cell pulverizer produced by Shanghai Zhixin Instrument Co., Ltd.

Embodiment 2

[0034] Weigh 6mg of strychnine, 400mg of soybean lecithin and 200mg of protein cholesterol, dissolve them in 20ml of propyl acetate, transfer them to a round bottom flask, and evaporate the organic solvent under reduced pressure at a water bath temperature of 35°C at a speed of 300r / min. Form a film and dry it under vacuum for 20 hours at room temperature. Then add 10ml of Span80 aqueous solution with a concentration of 1mg / ml and 90ml of phosphate buffer, adjust the pH to 8.0, swell for 1 hour, then ultrasonicate in a water bath for 10min, and then use an ultrasonic cell pulverizer to sonicate for 20min at a certain power. The strychnine liposome with white opalescence was obtained.

Embodiment 3

[0036] Weigh 168mg of strychnine, 400mg of egg yolk lecithin and 20mg of serum cholesterol and dissolve them in 100ml of isoamyl alcohol, transfer them to a round-bottomed flask, and evaporate the organic solvent under reduced pressure at a temperature of 40°C at a speed of 150r / min. Form a film and dry it under vacuum for 24 hours at room temperature. Then add 60ml of polyethylene glycol phosphate buffer with a concentration of 10mg / ml, adjust the pH to 8.5, fully swell for half an hour, then ultrasonicate in a water bath for 10min, and then use an ultrasonic cell pulverizer to sonicate at a certain power for 20min. The strychnine liposome with white opalescence was obtained.

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Abstract

The invention discloses a nanometer vauqueline liposome and preparation method which consists of dissolving strychnine, lecithin and cholestrin into organic solvent with a predetermined volume, removing organic solvent from the solution through depressed evaporation for film forming, charging microcosmic salt cushioning solution containing a predetermined concentration of surface active agent, and carrying out swelling, free assembling, ultrasonic dispersing. The prepared strychnine liposome suspending liquid can be used for treating various diseases including gout, arthritis and traumatic injury.

Description

technical field [0001] The invention relates to a nano strychnine liposome and a preparation method thereof. Background technique [0002] Strychnium is a perennial plant belonging to the Strychraceae. According to literature reports (Miao Xinrong et al. Clinical Application of Nuxebra, Xinjiang Traditional Chinese Medicine, 2000, 18(2): 61; Huang Xiru et al. Research Progress of Nuxebra, Shanghai Journal of Traditional Chinese Medicine, 2005, 39(1) : 62; Ying Senlin et al. Clinical application of Nuxebra chinensis, Journal of Tianjin University of Traditional Chinese Medicine, 2003, 22(1): 51; 21): 2200-2200.), Nuxychia has outstanding functions of dispelling stagnation, reducing swelling, dredging collaterals and relieving pain, etc. It is clinically used to treat various painful diseases such as gout, arthritis, bruises and other painful diseases. But at the same time, the clinical use of Nuxebra chinensis is restricted by various factors, mainly as follows: (1) It is h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/475A61P29/00A61K9/127
Inventor 刘昌胜袁媛杨柏灿
Owner EAST CHINA UNIV OF SCI & TECH
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