Gamma-polyglutamic acid hydrogel obtained by using enzymic method, and preparation method thereof

A polyglutamic acid and hydrogel technology, which is applied in the field of preparing hydrogel by cross-linking with glutamine transaminase, can solve the problems of complex production process, high equipment requirements, inconvenient operation, etc., and achieves low equipment requirements and high water absorption. Swellability, easy operation effect

Inactive Publication Date: 2013-12-04
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method is to use 60Co γ-ray crosslinking to prepare hydrogel, but this method requires high equipment and is inconvenient to operate
Chemical methods, that is, adding hydrophobic chemical crosslinking agents, such as diamines, haloalkanes, sulfides, epoxy resins, compound salts, and ethylene glycol glycidyl ether, etc., are prepared through a series of chemical reactions. Most of the joint agents are petroleum resources, and there will be certain safety problems when used in the field of biomedicine. In addition, the production process is complicated and the cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] γ -PGA is a commercially available food and pharmaceutical grade raw material with a molecular mass of 1.2 million Daltons, with a content of 99% (mass fraction); casein is a biochemical reagent with a content of 92%; TG enzyme is a commercially available food-grade enzyme reagent, It contains 1 % (mass fraction) enzyme and 99 % (mass fraction) maltodextrin.

[0016] Using pH 7.4 Tris / HCl buffer as solvent, prepare 1%~5% (mass fraction) γ -PGA solution and 10% (mass fraction) casein solution, in which casein solution should be dissolved by stirring in a water bath at 50°C overnight, mix 10 mL of each of the two prepared solutions, adjust the pH to 7.4, add 0.3% (mass fraction) ) of TG enzyme, after stirring and dissolving, put it in a water bath at 40 °C, take it out after 7 hours, take a boiling water bath for 5 minutes, and put it in the refrigerator at 4 °C for 15 hours to form a gel. Pour out the supernatant and use Distilled water was used to wash off the residua...

example 2

[0019] The source of raw materials is the same as that of Example 1.

[0020] Using pH 7.4 Tris / HCl buffer as solvent, prepare 10 % (mass fraction) casein solution, stir and dissolve in water bath at 50 °C overnight, take 16 mL of the prepared casein solution, add 1 mL ~ 5 mL of pH 7.4 Tris / HCl Buffer and 0.1g~0.5g γ -PGA powder, mix well, adjust the pH to 7.4, add 0.3% (mass fraction) TG enzyme, stir to dissolve, put it in a 40°C water bath, take it out after 6 hours, take a boiling water bath for 5 minutes, and put it in a refrigerator at 4°C After refrigerating for 15 hours, the gel can be formed. The supernatant is poured out, and the uncrosslinked components remaining on the surface of the gel are washed with distilled water, and then the gel is freeze-dried or air-dried at 50°C to obtain the gel. finished product. Immerse the dried gel in distilled water, filter out the unabsorbed water with 4 layers of gauze at regular intervals, and weigh to obtain the wet weight of ...

example 3

[0023] The source of raw materials is the same as that of Example 1.

[0024] Use Tris / HCl buffer with pH 7.4 as solvent to prepare 10% (mass fraction) casein solution, stir and dissolve in water bath at 50°C overnight, take 15mL~19mL of the prepared casein solution, add 1mL~5mL pH 7.4 Tris / HCl buffer and 1.67g ~ 2.11g γ -PGA powder, mix well, adjust the pH to 7.4, add 0.3% (mass fraction) TG enzyme, stir to dissolve, put it in a 40°C water bath, take it out after 6 hours, take a boiling water bath for 5 minutes, and put it in a refrigerator at 4°C After refrigerating for 15 hours, all the raw materials can be cross-linked to form a gel without residual supernatant, and then the gel can be freeze-dried or air-dried at 50°C to obtain a finished gel. Immerse the dried gel in distilled water, filter out the unabsorbed water with 4 layers of gauze at regular intervals, and weigh to obtain the wet weight of the gel. Repeat the operation until the water absorption of the gel reach...

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PUM

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Abstract

The invention relates to a method for preparing biodegradable super water absorbent gamma-polyglutamic acid (gamma-PGA) hydrogel. The method comprises the following steps: using transglutaminase (TG enzyme) as a crosslinking agent, and through acyl transfer reaction, performing crosslinking on gamma-PGA and casein protein to form composite protein super water absorbent hydrogel. The invention further relates to the biodegradable super water absorbent gamma-polyglutamic acid (gamma-PGA) hydrogel and application thereof. The method has the advantages that (1) the reaction conditions are mild, the time is short, the operation is simple and convenient, and requirements on equipment are low; (2) sources of raw materials are security, and the hydrogel is environmental-friendly and degradable; (3) lots of active sites are positioned inside the hydrogel, and can be combined with medicine to retain the activity of the medicine, so that an ideal biomedical material is provided; (4) the hydrogel has a stronger water absorption swelling property and a stable watering binding capacity.

Description

technical field [0001] The present invention relates to a gamma - A novel amino acid hydrogel with polyglutamic acid components, specifically relating to a method for preparing a hydrogel by cross-linking with transglutaminase and the application of the hydrogel. Background technique [0002] In recent years, hydrogels have been widely used in food biology, agriculture, forestry and horticulture, petrochemical industry, medical and health care, etc. However, at present, most of the materials for preparing hydrogels are chemically synthesized. Not only the raw materials and preparation will cause certain pollution to the environment, but also the prepared products will decompose slowly in nature, which will also cause pollution to the environment. In addition, with the increasing application of hydrogels in the field of biomedicine, the research on biodegradable hydrogels has been promoted due to the special requirements of biomedical materials. [0003] Produced by microbi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08L77/04C08L89/00A61K47/42A61K47/34A23L1/228A23L1/30A23L1/305A23L33/175
Inventor 惠明高春媛田青赵红月黄亮夏克东黄继红
Owner HENAN UNIVERSITY OF TECHNOLOGY
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