The invention provides an herbal enzyme. The enzyme comprises fruit raw materials and traditional Chinese medicine raw materials. The fruit raw materials comprise the following components of, by weight, 1-3 parts of strawberries, 1-3 parts of honey-dew melons, 1-3 parts of blueberries, 1-5 parts of pears, 1-6 parts of litchis, 2-5 parts of kiwifruit, 2-5 parts of grapes, 1-4 crataegus, 1-3 parts of watermelons, 1-3 parts of muskmelons, 1-4 parts of bananas, 2-5 parts of citrus maxima, 2-3 parts of apples, 1-3 part of pomegranates, 1-4 parts of cherries, 1-4 parts of peaches, 1-5 parts of Chinese dates, 2-6 parts of pineapples and 3-6 parts of papayas. The traditional Chinese medicine raw materials comprise the following components of, by weight, 2-5 parts of panax pseudoginseng var. notoginseng, 0.06-0.1 part of cordyceps sinensis, 2-4 parts of radix isatidis, 3-3.5 parts of roses, 1-4 parts of dioscorea opposite, 3-6 parts of dendrobium officinale, 2-6 parts of chrysanthemum, 2-5 parts of mahonia fortune, 1-4 parts of pyrrosia lingua and 1-6 parts of glycyrrhiza uralensis. The enzyme is fermented with the fruits and the Chinese herbal medicine as the raw materials, so that the obtained enzyme comprises the diversified components, and the healthcare function of the enzyme is improved; and moreover, the enzyme can meet the function of medicined diet and nourishing food and can be directly ate by customers. In addition, the invention discloses a preparation method of the herbal enzyme.