Dendrobium small molecule peptide and preparation method thereof

A technology for small molecule peptides and dendrobium, which is applied in complex biological enzyme digestion and biochemical separation process, small molecule peptides of dendrobium and its application fields, can solve the problems of complicated post-processing steps, unfavorable mass production, increased process time, etc., and saves money Enzymatic hydrolysis time, easy cleaning and regeneration, reduction of ash and salt generation effect

Inactive Publication Date: 2017-12-08
陈玫辛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the above-mentioned patented technology, the peptides prepared by the single enzyme are often of poor product quality, and multiple enzymes are used for enzymatic hydrolysis. The cost is high and the process time is greatly increased. The post-processing steps are cumbersome, which is not conducive to mass production.

Method used

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  • Dendrobium small molecule peptide and preparation method thereof
  • Dendrobium small molecule peptide and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of the dendrobium small molecular peptide is to obtain the dendrobium small molecular peptide product through pretreatment, extraction, enzymolysis, separation and purification, concentration, drying and refining.

[0031] 1) Fresh Dendrobium nobile is crushed and refined to obtain a slurry;

[0032] 2) Adding water with a mass 5 times the mass of the slurry, heating to 40°C, and ultrasonically extracting for 30 minutes with an ultrasonic power of 30kW to obtain an extract;

[0033] 3) Heat the extract to 50°C, first add 0.08% xylanase and 0.01% cellulase in a mass ratio to the extract to hydrolyze for 1 hour, then add 0.1 mass ratio to the extract The alkaline protease of %, the plant protease of 0.1% carry out secondary enzymolysis, and enzymolysis time is 2 hours; Described plant protease is made of papain, bromelain and actinidia protease, and the mass ratio of three kinds of enzymes is 4:3: 3.

[0034] 4) Heat the enzymolysis solution to 85...

Embodiment 2

[0039] The preparation method of the dendrobium small molecular peptide is to obtain the dendrobium small molecular peptide product through pretreatment, extraction, enzymolysis, separation and purification, concentration and drying.

[0040] 1) Fresh Dendrobium nobile is crushed and refined to obtain a slurry;

[0041] 2) Add water with a mass three times the mass of the slurry, heat to 60°C, perform ultrasonic extraction for 20 minutes, and the ultrasonic power is 30kW to obtain an extract;

[0042] 3) Warm the extract to 52°C, first add 0.1% xylanase and 0.02% cellulase in a mass ratio to the extract for 0.5 hours, and then add a mass ratio of 0.15 to the extract The alkaline protease of %, the plant protease of 0.15% carry out secondary enzymolysis, and enzymolysis time is 2.5 hours; Described plant protease is made of papain, bromelain and actinidia protease, and the mass ratio of three kinds of enzymes is 4:3: 3.

[0043] 4) Heating the enzymolysis solution to 85°C for...

Embodiment 3

[0048] The preparation method of the dendrobium small molecular peptide is to obtain the dendrobium small molecular peptide product through pretreatment, extraction, enzymolysis, separation and purification, concentration and drying.

[0049] 1) Fresh Dendrobium nobile is crushed and refined to obtain a slurry;

[0050] 2) adding water with a mass 4 times the mass of the slurry, heating to 80°C, and ultrasonically extracting for 10 minutes with an ultrasonic power of 30kW to obtain an extract;

[0051] 3) Heat the extract to 55°C, first add 0.12% xylanase and 0.05% cellulase to react for 0.5 hours in mass ratio to the extract, and then add 0.2% mass ratio to the extract The alkaline protease of %, the plant protease of 0.25% carry out secondary enzymolysis, and enzymolysis time is 1.5 hours; Described plant protease is made of papain, bromelain and actinidia protease, and the mass ratio of three kinds of enzymes is 4:3: 3.

[0052] 4) Heating the enzymolysis solution to 102° C...

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Abstract

The invention provides a preparation of dendrobium small molecule peptides and dendrobium small molecule peptides obtained therefrom. The preparation method comprises the following steps: 1) pretreatment; 2) extraction; 3) enzymolysis; 4) separation and purification; and 5) concentration and drying. The enzymolysis time is shortened to 2-3h by way of compound biological enzyme digestion on xylanase, cellulase, alkaline protease and plant protease. The pH value needs not to be adjusted during enzymolysis, so that the process time is shortened, and meanwhile, the desalting process is canceled, and the production efficiency of the dendrobium small molecule peptide is increased. The molecular weight of the dendrobium small molecule peptides obtained by the preparation method is primarily at 100-500Da, so that the dendrobium small molecule peptides are easily absorbed by a human body and can be applied to the field of food.

Description

technical field [0001] The invention belongs to the technical field of biology and new medicine, and relates to a preparation method for extracting dendrobium small molecular peptides by using a compound biological enzymatic digestion and biochemical separation process using dendrobium as a raw material. In addition, the present invention also relates to the dendrobium small molecular peptide prepared by the above method and its application. Background technique [0002] Dendrobium is a traditional precious Chinese medicine in my country, listed as top grade in "Shen Nong's Herbal Classic". Dendrobium dendrobium nature and flavor are sweet and slightly salty, cold, return stomach, kidney, lung meridian. Tonify stomach and promote body fluid, nourish yin and clear away heat. [0003] Dendrobium mainly contains active ingredients such as dendrobium polysaccharides, alkaloids, amino acids, trace elements, and phenanthrene compounds. Nearly half of the amino acids are essentia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23L33/18
CPCA23V2002/00A23L33/18C07K1/34C12P21/06A23V2250/55
Inventor 陈玫辛田新风
Owner 陈玫辛
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