Method for preparing silkworm chrysalis source ACE (Angiotensin Converting Enzyme) inhibitory peptide by ultrasonic-ionic liquid coupling technology

A technology of ionic liquids and ultrasound, applied in the field of bioengineering, can solve the problems of preparing silkworm chrysalis-derived ACE inhibitory peptides, and achieve the effects of shortened enzymatic hydrolysis time, low raw material prices, and simple production

Active Publication Date: 2014-02-12
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Ultrasonic-ionic liquid coupling technology has been used in biocatalytic reactions, such as the Chinese patent "A method for ultrasonic-assisted ionic liquid to catalyze microalgae oil to produce biodiesel (publication number: CN102732385A)"; Coupling report on preparation of ACE inhibitory peptide from silkworm chrysalis

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  • Method for preparing silkworm chrysalis source ACE (Angiotensin Converting Enzyme) inhibitory peptide by ultrasonic-ionic liquid coupling technology

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Effect test

Embodiment 1

[0024]According to the ratio of liquid to material 20mL / g, the silkworm chrysalis protein was prepared into a suspension with distilled water, and then mixed with an equal volume of ionic liquid and added to the beaker. The 24kHz ultrasonic probe was immersed in the water phase, and the ultrasonic wave was turned on. The pretreatment temperature was controlled at 25°C. The ultrasonic intermittent ratio is 1:1, the ultrasonic power is 300W, and the pretreatment time is 20min. After the pretreatment, centrifuge to collect the silkworm pupae protein and recover the ionic liquid. The collected silkworm pupae protein is washed with distilled water and centrifuged until the residual ionic liquid is removed. , the pretreated silkworm chrysalis protein was prepared into a solution with a substrate concentration of 1.0% (w / v, g / mL) with distilled water, under the conditions of enzymolysis temperature 45°C, enzymolysis pH 8.0, and enzyme addition 2000U / g Hydrolyze with alkaline protease ...

Embodiment 2

[0026] According to the ratio of liquid to material of 27mL / g, the silkworm chrysalis protein was prepared into a suspension with distilled water, and then mixed with an equal volume of ionic liquid and added to the beaker. The 24kHz ultrasonic probe was immersed in the water phase, and the ultrasonic wave was turned on. The pretreatment temperature was controlled at 30°C. The ultrasonic intermittent ratio is 1:1, the ultrasonic power is 406W, and the pretreatment time is 32min. After the pretreatment, centrifuge to collect silkworm chrysalis protein and recover the ionic liquid. The collected silkworm chrysalis protein is washed with distilled water and centrifuged until the residual ionic liquid is removed. , the pretreated silkworm chrysalis protein was prepared into a solution with a substrate concentration of 2.5% (w / v, g / mL) with distilled water, under the conditions of enzymolysis temperature 50°C, enzymolysis pH 8.5, and enzyme addition 3000U / g Hydrolyze with alkaline p...

Embodiment 3

[0028] According to the ratio of liquid to material 40mL / g, the silkworm chrysalis protein was prepared into a suspension with distilled water, and then mixed with an equal volume of ionic liquid and added to the beaker. The 24kHz ultrasonic probe was immersed in the water phase, and the ultrasonic wave was turned on. The pretreatment temperature was controlled at 35°C. The ultrasonic intermittent ratio is 1:1, the ultrasonic power is 500W, and the pretreatment time is 40min. After the pretreatment, centrifuge to collect silkworm chrysalis protein and recover the ionic liquid. The collected silkworm chrysalis protein is washed with distilled water and centrifuged until the residual ionic liquid is removed. , the pretreated silkworm chrysalis protein was prepared into a solution with a substrate concentration of 5.0% (w / v, g / mL) with distilled water, under the conditions of enzymolysis temperature 55°C, enzymolysis pH 9.0, and enzyme addition 4000U / g Hydrolyze with alkaline prot...

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Abstract

The invention discloses a method for preparing a silkworm chrysalis source ACE (Angiotensin Converting Enzyme) inhibitory peptide from by an ultrasonic-ionic liquid coupling technology. The method comprises the following steps: preparing silkworm chrysalis protein into a suspension by using distilled water, subsequently mixing the suspension with an isopyknic ionic liquid and adding into a flask, dipping an ultrasonic probe into a water phase so as to perform ultrasonic-ionic liquid coupling treatment, after the treatment is accomplished, centrifuging, collecting the silkworm chrysalis protein and recycling the ionic liquid, firstly washing the collected silkworm chrysalis protein and subsequently centrifuging so as to remove the residual ionic liquid, and preparing the ACE inhibitory peptide from pretreated silkworm chrysalis protein by using an enzyme method. Compared with the conventional untreated method, the method for preparing the silkworm chrysalis source ACE inhibitory peptide by an ultrasonic-ionic liquid coupling technology remarkably improves the activity of the ACE inhibitory peptide, that is, the activity is increased by 34-54%.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a production method of functional food and biopharmaceuticals, in particular to a method for preparing silkworm chrysalis-derived ACE inhibitory peptides by coupling ultrasonic waves and ionic liquids. Background technique [0002] Hypertension is known as the "silent killer" internationally. At present, there is no specific medicine that can cure high blood pressure, and patients with high blood pressure need to take medicine for life. Studies have found that angiotensin-I converting enzyme (ACE) is considered to be the key enzyme leading to high blood pressure in the human body. - The prostaglandin system plays an important role in the regulation of blood pressure. Therefore, by inhibiting ACE activity, the blood pressure of hypertensive patients can be effectively controlled to normal levels. More than 20 kinds of ACE-inhibiting antihypertensive drugs such as captopril, enalapril...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 贾俊强吴琼英桂仲争颜辉
Owner JIANGSU UNIV OF SCI & TECH
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