38results about How to "Improve typicality" patented technology

The invention relates to a method for establishing fingerprint maps of rhaponticum uniflorum of Mongolian medicines. The method includes 1), preparing test solution, to be more specific, arranging medicinal material powder in a 100 mL conical flask with a stopper, adding 70% methanol into the conical flask, carrying out microwave extraction, arranging the conical flask in a room-temperature environment, then weighing the conical flask, supplementing lost weight of the conical flask by the aid of solvents, filtering liquid to obtain filter liquid and filtering the filter liquid by the aid of filter membranes with micro-pores with the sizes of 0.45 micrometer; 2), filling an efficient liquid-phase chromatographic instrument with the test solution to obtain the fingerprint maps. Chromatographic conditions includes that chromatographic columns are octadecylsilane chemically bonded silica chromatographic columns; mobile phases include acetonitrile (A) and 0.2% phosphoric acid aqueous solution (B), and the B is eluted by 85% (for 0 min), 78% (for 12 min), 74% (for 13 min) and 60% (for 60 min) in a gradient manner. Detection conditions include that the detection wavelengths of DAD (diode array detectors) are 230-400 nm; column temperatures are 20-40 DEG C; the sample sizes are 5-30 micro-L. The invention further provides a method for evaluating the quality of the rhaponticum uniflorum of the Mongolian medicines by the aid of the fingerprint maps. The methods have the advantages that the methods are high in sensitivity and good in repeatability; the varieties and the quantities of chemical components in Mongolian medicinal materials with the rhaponticum uniflorum can be comprehensively reflected by the aid of the obtained standard fingerprint maps.

The invention discloses a method for improving flavor of snowflake pear juice by use of composite glycosidase, wherein the snowflake pear juice is adopted as a raw material. According to the method disclosed by the invention, potential aromatic substances in the juice can be released, thus the loss of aroma substances is reduced in a juice processing process, the aroma quality is improved, and the product typicality is enhanced. After aroma enhancement by the composite glycosidase, the juice aroma is stronger, the mouthfeel is harmonious, and the market potential is huge.

The invention relates to a secondary fermentation process of blueberry fruit wine. The secondary fermentation process comprises the following steps: raw material sorting, crushing, and pulping; enzymolysis; juice squeezing; freezing concentration; ice yeast main fermentation and fruit wine yeast secondary fermentation; pot pouring, clarification and filtration; ageing; sterilization, cooling and filtration; and storage, filtration and encapsulation. By utilizing a ice yeast main fermentation-fruit wine yeast secondary fermentation technique, the concentration of a dry extract of the blueberry fruit wine is increased, the generation amount of grain oil in a fermentation process is reduced, and the typicality of the finished wine is improved.

The invention discloses grape distilled wine and a preparation method thereof. The preparation method includes: using vitis vinifera variety merlot as a raw material, and performing crushing and squeezing, low-temperature fermenting and one-time distillation to obtain distilled raw wine; aging, freezing, filtering and filling to obtain grape distilled wine of 62.0+ / -1.0%vol. One-time distillation is adopted to replace two-time distillation, so that energy consumption and distillation time are reduced. No additive is used in the whole production process of the distilled wine. The grape distilled wine obtained by the method has aroma of grape red berry, has mellow aroma endowed by the distillation process, is harmonious in wine body and presents fruity aroma and typical performance more sufficiently.

The invention discloses a device and method for extracting the total DNA of substrate-attached microorganisms, belonging to the technical field of molecular biology. The device includes a pressure-controlled flushing device, a negative pressure device and a collection device. The pressure of the flushing water is controlled by the pressure-controlled flushing device. The suspension produced by flushing the matrix through the negative pressure pipeline is collected to the collection device. The invention also discloses a DNA extraction method. The matrix is ​​washed under pressure, and the sample is collected with a negative pressure device, which overcomes the prior art. Aiming at the difficulty and incomplete sampling of microbial samples attached to the matrix, the representativeness, extraction efficiency and quality of the microbial genomic DNA attached to the matrix have been significantly improved, the bands are bright and clear, and there is basically no degradation. Fungi, etc. have good extraction efficiency.