A device and method for extracting the total DNA of substrate-attached microorganisms

A technology of microorganisms and substrates, applied in the field of molecular biology, can solve the problems of poor representativeness of results, rupture, poor representativeness of microorganisms, etc., and achieve the effects of improved representativeness, good quality and improved typicality
CN111269800BActive Publication Date: 2021-06-22NANJING UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING UNIV
Publication Date
2021-06-22

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Abstract

The invention discloses a device and method for extracting the total DNA of substrate-attached microorganisms, belonging to the technical field of molecular biology. The device includes a pressure-controlled flushing device, a negative pressure device and a collection device. The pressure of the flushing water is controlled by the pressure-controlled flushing device. The suspension produced by flushing the matrix through the negative pressure pipeline is collected to the collection device. The invention also discloses a DNA extraction method. The matrix is ​​washed under pressure, and the sample is collected with a negative pressure device, which overcomes the prior art. Aiming at the difficulty and incomplete sampling of microbial samples attached to the matrix, the representativeness, extraction efficiency and quality of the microbial genomic DNA attached to the matrix have been significantly improved, the bands are bright and clear, and there is basically no degradation. Fungi, etc. have good extraction efficiency.
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Description

technical field

[0001] The invention relates to the technical field of molecular biology, in particular to a device and a method for extracting the total DNA of substrate-attached microorganisms. Background technique

[0002] In recent years, with the development of sequencing technology, molecular biology methods for analyzing microbial diversity and function based on total deoxyribonucleic acid (DNA) in environmental samples have been continuously improved, including fluorescent quantitative nucleic acid amplification of a specific gene Detection (qPCR) method, metagenomic technology for sequencing the total DNA of microorganisms in the environment, etc., have gradually been widely used. Metagenomic technology is recognized as the most comprehensive and accurate method for studying environmental microbial community structure and functional genes, because it has no special selectivity (such as pure culture / enrichment), and it also reduces the preference of sequencing techno...

Claims

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