25 results about "DNA degradation" patented technology

Abstract Described herein, in one aspect, are antigen presenting cells (APCs) comprising an exogenous nucleic acid encoding one or more candidate antigens, wherein the one or more candidate antigens are expressed and presented with MHC class I or MC class II molecules; a molecular reporter of Granzyme B (GzB) activity; and c) an exogenous inhibitor of caspase-activated deoxyribonuclease (CAD)-mediated DNA degradation, a CAD knockout, or a caspase knockout (e.g., caspase 3 knockout). Described herein, in another aspect, is a system for detection of recognized antigen presentation by an antigen presenting cell to a cytotoxic lymphocyte or NK cell. Abstract 2018 / 22761 oM - cell Target ml Target cell cell cell Target Target Target SUBSTITUTE SHEET (RULE 26) cell cell cell Target Target cell cell 1 / 28 my Isolate recognized cell Library of target cells target cells and displaying different Add T cells from sample sequence antigens antigens of interest. CTLs deliver cytotoxic granules to target cells displaying cognate antigen FIG. 1 PCT / US2018 / 036663 20 26 20 53 56 07 J ul 2 02 6 2 0 2 6 2 0 5 3 5 6 0 7 J u l 2 0 2 6 2 0 1 8 / 2 2 7 6 1 o M a n d m y 1 / 2 8 m y L i b r a r y o f t a r g e t c e l l s d i s p l a y i n g d i f f e r e n tA d d T c e l l s f r o m s a m p l e of interest. CTLs deliver c y t o t o x i c g r a n u l e s t o t a r g e t c e l l s d i s p l a y i n g c o g n a t e a n t i g e n P C T / U S 2 0 1 8 / 0 3 6 6 6 3

The invention relates to the technical field of river biological monitoring, and mainly discloses a Yellow River basin aquatic organism diversity monitoring system, which comprises a suspension unit comprising a buoy assembly and a power supply assembly arranged on the buoy assembly; the collecting unit comprises a collecting barrel and a cleaning assembly, the collecting barrel and the cleaning assembly are arranged at the bottom of the buoy assembly, and the outer wall of the collecting barrel makes contact with the cleaning assembly; the fixing unit comprises a driving box and an anchor hook, the driving box is arranged at the bottom of the cleaning assembly, and the anchor hook is fixed to a driving set in the driving box; the problems that in an existing aquatic organism diversity monitoring system, a collecting device lacks an intelligent timing recycling mechanism, so that a sample is prone to rot after being soaked for a long time, DNA degradation is caused, and morphological characteristics are damaged are solved.

The invention provides a seaweed field sediment eDNA sampling method based on in-situ immobilization, and belongs to the technical field of seaweed field sediment detection.The method includes the steps that insertion parameters are adaptively adjusted by applying a vibration strategy optimization model in the seaweed field sediment sampling process, winding and grounding states are judged by inserting a state recognition model, and therefore the sampling accuracy of seaweed field sediment eDNA is improved; sampling compression is eliminated through a sediment compression self-adaptive compensation algorithm, and an optimal injection pressure curve is calculated through a multiphase flow fixing agent diffusion optimization algorithm to drive a fixing agent to uniformly cover an effective area within preset diffusion time to complete in-situ fixation. The technical problem that the DNA degradation rate is high due to the fact that a fixing agent cannot uniformly permeate in situ in the seaweed field sediment eDNA sampling process is solved.

The invention belongs to the technical field of nano material preparation and application, and discloses a method for liquid-phase stripping of iron oxychloride by cysteine and application. The method comprises the following steps: firstly, preparing blocky iron oxychloride through partial thermal decomposition of ferric chloride hexahydrate; and adding L-cysteine into the iron oxychloride solution, and carrying out ultrasonic treatment at 20-40kHz and 10-40 DEG C for 0.5-6 hours to finally prepare the cysteine-iron oxychloride nano material. The average size of the material is 20-200nm, and the material is strip-shaped, is rich in ferrous iron and oxygen vacancies, and has excellent peroxidase-like activity and Fenton reaction activity. The method is green, simple, convenient and low in cost. The prepared nano material can efficiently catalyze and generate active oxygen species such as hydroxyl free radicals, effectively kills drug-resistant escherichia coli and staphylococcus aureus through multiple mechanisms such as initiation of bacterial lipid peroxidation, DNA degradation and protein leakage consumption, shows good biocompatibility in a cytotoxicity test, and can be used for preparing a drug-resistant nano material. Wide application prospects are realized in the field of antibacterial preparations.

The utility model discloses a trace plant specimen DNA (deoxyribonucleic acid) adsorption and collection device, which belongs to the technical field of molecular biology and comprises a base, a grinding bowl is fixedly connected to the upper end of the base, and a first blanking hole and a second blanking hole are respectively formed in the bottom end of the inner wall of the grinding bowl. According to the scheme, powdery plant tissue is pushed into the second collecting tank or the first collecting tank from the interior of the grinding bowl, then powder in the powdery plant tissue is poured into the centrifugal tube at a time to be collected, and compared with the prior art that a digging spoon is used for transferring for multiple times, the powder collecting device has the advantages that the powder collecting device is simple in structure and convenient to use. Compared with the prior art, the extraction device has the advantages that the extraction speed is obviously increased, the retention time of plant tissues in air can be effectively shortened, the extraction speed is increased, the degradation degree of DNA (deoxyribonucleic acid) is reduced, the amount of extracted powder can be accurately controlled within two intervals of 50-200mg and 15-50mg through the second collection tank and the first collection tank, and convenience is brought to extraction work.

The application relates to the technical field of biological sample preservation, in particular to a sample preservation reagent for InDel molecular markers of Pachyhynobius and a preparation method thereof. Through multiple action mechanisms such as maintaining pH stability by a buffer system, inhibiting nuclease activity by a nucleic acid protective agent, and removing free radicals by an antioxidant, the DNA degradation in the preservation process can be significantly reduced, the integrity of the DNA is ensured, and a reliable template is provided for the accurate detection of subsequent InDel molecular markers. The use of a cell stabilizer can form a protective film around the cells, prevent ice crystal damage to the cells, and maintain the osmotic pressure balance of the cells, so that the cells can maintain a high activity in the preservation process, and high-quality DNA can be extracted from the cells in the subsequent process; the addition of an antibacterial agent can effectively inhibit the possible microbial contamination of the sample in the preservation process, prolong the preservation time of the sample, and ensure the quality and safety of the sample.

The application provides an environmental DNA extraction method and device. The environmental DNA extraction device comprises an upper seat body, a lower seat body and a detachable membrane assembly. The upper seat body is a column with a sealed upper end and an open lower end. Tangential flow water inlets and tangential flow water outlets are arranged on the two side walls of the upper seat body. The upper end of the lower seat body is open, and the lower end of the lower seat body is provided with a filtrate outlet. The lower end of the upper seat body is connected to the upper end of the detachable membrane assembly, and the upper end of the lower seat body is connected to the lower end of the detachable membrane assembly. The detachable membrane assembly is arranged between the upper seat body and the lower seat body. Environmental water flows into the detachable membrane assembly through the tangential flow water inlets. The detachable membrane assembly filters and adsorbs the environmental water to adsorb environmental DNA in the environmental water on the detachable membrane assembly, and the remaining filtrate flows out through the filtrate outlet of the lower seat body. The extraction process can be simplified, the time consumption can be reduced, the risk of DNA degradation and sample pollution can be avoided, and the DNA extraction quality can be improved.

The invention discloses a phase separation mediated nano trap for removing extracellular free DNA (Deoxyribonucleic Acid) as well as a preparation method and application thereof, and relates to the technical field of medical nano biological materials. The nano trap takes hollow mesoporous cerium dioxide as a core, DNA degrading enzyme is loaded in the nano trap through a coordinate bond, and a cationic polymer layer is modified on the surface of the nano trap, so that the nano trap has positive zeta potential. The structure can be used as a nucleation center to efficiently capture negatively charged cfDNA; the cfDNA is mediated to generate liquid-liquid phase separation to form a stable aggregate, and then the cfDNA is subjected to in-situ degradation through a loaded enzyme. The invention further discloses a construction method of the nano trap and application of the nano trap in preparation of drugs for treating or preventing systemic inflammatory response syndromes (such as septicopyemia) mediated by abnormal cfDNA level rise. The nano trap provided by the invention has the advantages of high cfDNA removal efficiency, innovative mechanism, synergistic effects of oxidation resistance and bacteria resistance and the like, and provides an effective new way for treatment of related diseases.

The invention belongs to the technical field of biology, and particularly discloses a library construction method for combined analysis of histone modification and DNA methylation and application. The method comprises the following steps: assembling fusion transposase with antibody binding activity and a first joint modified by methylated cytosine to obtain a transposase compound, specifically positioning the transposase compound to a target histone modification site for in-situ targeting fragmentation, and introducing the first joint to one end of the obtained DNA fragment; carrying out dephosphorylation and conversion treatment on the DNA fragment with the first linker, introducing a second linker to the other end of the treated DNA fragment, and carrying out amplification to construct a sequencing library; histone modification information and DNA methylation information can be obtained at the same time through sequencing and data analysis. According to the method, information loss caused by DNA degradation can be reduced to the maximum extent, the PCR repetition rate is reduced, high-resolution and multi-dimensional chromatin analysis is achieved with fewer cell samples, and data integrity is improved.

The invention discloses a composition and method for cell preservation and DNA extraction of NGS detection, and relates to the technical field of biological sample treatment.The composition is prepared from a cell sample preservation solution, a solid phase, a binding solution, a washing solution and an eluent. The preservation solution can avoid cell solidification and nucleic acid and protein cross-linking, reduce DNA degradation and false positive risks, and significantly improve DNA extraction quality, and meanwhile, the preservation solution has a lysis function, a sample can be stably preserved for 3-30 days at 4-30 DEG C without low-temperature conditions, additional lysis steps and protease are not needed during subsequent extraction, the operation can be completed by directly combining with a solid phase, the process is greatly simplified, and the cost is reduced. The efficiency is improved, and a reliable DNA template is provided for high-precision molecular diagnosis such as NGS.

The invention relates to the technical field of sequencing, in particular to a preparation method of a sequencing library and a reagent. The invention provides a preparation method of a sequencing library, which comprises the following steps of: additionally adding an additive or an additive combination capable of reducing a DNA secondary structure and / or reducing single-stranded DNA degradation in single-stranded cyclization reaction and / or rolling circle amplification reaction; an additive or an additive combination capable of reducing a DNA secondary structure, reducing single-stranded DNA degradation and / or reducing double-stranded DNA double-helix stability is additionally added in a double-stranded cyclization library rolling circle amplification reaction, so that the binding capacity of DNA ligase and / or strand displacement DNA polymerase and target nucleic acid is improved, and the situation that the rolling circle amplification efficiency of different circular DNAs is not uniform due to the secondary structure is reduced; the probability that the loose single-stranded DNA is easy to degrade is reduced, and the amplification uniformity of different GC regions and different sequence characteristic regions of the library is improved, so that the sequencing quality and the sequencing data yield are improved.

The invention discloses a method for identifying authenticity of DNA (Deoxyribose Nucleic Acid) degradation type bulbus fritillariae cirrhosae based on a digital PCR (Polymerase Chain Reaction) technology. The method comprises the following specific steps: S1, extracting DNA of a sample to be detected; s2, by taking the DNA as a template, carrying out digital PCR amplification reaction by utilizing a specific primer pair and a probe designed in an ITS2 region; and S3, according to the result of the digital PCR, identifying the fritillaria species of the sample to be detected. The invention provides a method which is high in sensitivity and strong in specificity, can directly and qualitatively detect the degradation of the DNA of the bulbus fritillariae cirrhosae, and can be used for quickly and accurately identifying the authenticity of the bulbus fritillariae cirrhosae.

The application discloses a detection primer combination for DNA degradation degree based on a micro-droplet digital PCR and application thereof, and belongs to the technical field of molecular biology. The detection primer combination comprises a primer pair and a probe for detecting a 235bp target, a primer pair and a probe for detecting a 145bp target, and a primer pair and a probe for detecting a 75bp target. The application utilizes digital PCR technology to detect three targets with different lengths, i.e., a large target, a medium target and a small target, and utilizes the number of the three targets to accurately evaluate the DNA degradation degree, help determine a subsequent STR typing method, and provide a new simple, economical, rapid and effective technical means for evaluation and detection of highly degraded forensic samples.

The invention relates to a low-temperature pollution-free storage and transportation device for lake sediment eDNA samples, and belongs to the technical field of environmental DNA detection. Comprising a container refrigerator, a supporting beam, a sliding seat, a V-shaped supporting roller and a pressing device, the supporting beam is longitudinally installed in the container refrigerator, the sliding seat is installed on the supporting beam in a sliding mode, the V-shaped supporting roller is installed on the sliding seat through a bearing in a free rotating mode, and the pressing device is installed on the sliding seat; the rock core tube and the sample in the rock core tube can be subjected to continuous low-temperature refrigeration and transportation, so that the DNA damage in the transportation process is reduced to the maximum extent, the DNA degradation is slowed down, and the introduction of exogenous DNA pollution in the sample collection and transportation process is reduced; the V-shaped supporting rollers are pushed and pulled to rotate in the loading and unloading process, so that the loading and unloading labor intensity can be effectively reduced; the V-shaped supporting rollers are matched with the pressing device to press and fix the rock core pipe, so that the stability in the transportation process is ensured; the storage and transportation of the rock core pipe sample are facilitated, and the storage time is prolonged.

The present invention discloses a multiplex amplification system for forensic human DNA detection by qPCR and its application. The multiplex amplification system simultaneously amplifies four targets, namely HS, HL, HY and IPC, by qPCR. Among them, the HS target is used for total DNA quantification, the HL target is used for degradation degree evaluation, the HY target is used for sex ratio judgment, and the IPC target is used for inhibitor detection. The kit of the present invention detects four targets, namely HS, HL, HY and IPC, by qPCR technology to achieve DNA quantification and quality analysis, and the operation is simple. It has high sensitivity (the lowest detection limit is 1 pg / μL), strong anti-inhibition ability, good specificity, can accurately quantify male and female DNA, can also evaluate the DNA degradation degree, judge PCR inhibitors, is applicable to a variety of biological samples, provides an efficient and accurate solution for forensic DNA detection, and helps solve cases.

Provided herein are compositions and methods to protect cargo DNA when it is loaded into lipid nanoparticles (LNPs) or similar nanocarrier systems. In certain embodiments, the compositions include a lipid nanoparticle (LNP) that includes a DNA cargo and a molecule capable of condensing and / or compacting DNA. In certain embodiments, the compositions also include a "helper molecule" that provides reduced inflammatory response and reduced DNA degradation by DNAse enzymes and autophagy / xenophagy during and after endosomal escape, upon administration.

The present application relates to the field of biotechnology and molecular diagnosis, and particularly relates to a methylation detection pretreatment kit and method for protecting DNA integrity, which can solve the problem that DNA degradation is serious in traditional bisulfite conversion technology, and conversion efficiency and DNA integrity are difficult to be obtained simultaneously, the kit comprises a conversion solution, a protection solution, a combination solution, a desulfonation solution, a washing solution and an elution solution, the conversion solution contains 2.5-4 mol / L bisulfite and linear polyethylene polyamine, the protection solution contains an organic denaturant and a polyphenol antioxidant, the DNA melting temperature is reduced by the organic denaturant, the linear polyethylene polyamine accelerates the conversion reaction, and the desulfonation system with low water activity is combined to complete the bisulfite conversion under mild conditions of 50-70 DEG C; the present application can realize the conversion efficiency of greater than 99.5% of unmethylated cytosine, greatly reduce DNA degradation, significantly improve the long fragment DNA recovery rate and the detection sensitivity of trace samples, and is suitable for the methylation detection of three generations of long read sequencing and clinical trace samples.

The invention provides a DNA extraction method and application thereof in multi-chromosome copy number variation analysis, and relates to the technical field of biomedical detection. The DNA extraction method provided by the invention comprises the steps of sample pretreatment, splitting decomposition, DNA adsorption and the like, and the DNA degradation rate of the FFPE sample is controlled below 15%. The detection system based on the extraction method comprises a wet experiment detection module, a biological information analysis module and a clinical decision module, wherein the wet experiment detection module executes the DNA extraction process; the biological information analysis module performs CNV detection through a dynamic sliding window algorithm, and calculates a Z value of a chromosome arm after GC correction and baseline normalization processing; the clinical decision module integrates MaxZ (maximum Z value) and CountZ (abnormal chromosome count) indexes and clinical variables to construct a model, and generates a risk layering report. The method and the system are suitable for multi-chromosome copy number variation analysis, and detection accuracy and clinical application value are improved.

The invention discloses a DNA macro bar code primer group for identifying animal species in pet food, a kit and application of the DNA macro bar code primer group and the kit, and relates to the technical field of molecular biology. The primer group comprises two pairs of primers 12S rRNA and 16S rRNA, forward and reverse sequences of the primer group are respectively specific basic group arrangement, and the primer group can cover mammals, poultry and fish species. During application, pet food DNA (deoxyribonucleic acid) is extracted, PCR (polymerase chain reaction) amplification is carried out by the primer group (12S and 16S primers respectively adopt specific amplification procedures), after Sanger sequencing, comparison with an NCBI (National Center of Biotechnology Information) database is carried out, and species are determined by taking 98% sequence similarity as a threshold value. The primer group can deal with the DNA degradation problem caused by high processing, the detection sensitivity and accuracy are improved, and an effective technical means is provided for pet food quality control.

The invention relates to a medical nano biological material, in particular to a preparation method and application of high-activity DNase-I nanoparticles capable of being inactivated in a light-operated mode. Strong electrostatic interaction occurs between a sulfonic negative charge group on a photosensitizer molecule and a positively charged region on the surface of DNase-I protein under the assistance of hydrogen bonds and hydrophobic interaction forces, so that the efficient photosensitizer and DNase-I co-loaded nanoparticles are formed, and the DNA degradation activity of DNase-I is remarkably enhanced. After nucleic acid pollutants in the environment are efficiently removed by the DNase-I nanoparticles, the loaded photosensitizer can be activated through illumination with specific wavelength such as an LED light source, and the active center of the DNase-I nanoparticles is irreversibly damaged by reactive oxygen species (ROS) generated by the photosensitizer, so that the activity of residual enzyme is thoroughly eliminated, and the problem of nuclease pollution is effectively solved.

The invention discloses an encoding gene of bacteriophage DNA enzyme, the nucleotide sequence of the encoding gene is shown as SEQID N0: 1, a recombinant vector containing the encoding gene of the bacteriophage DNA enzyme is transferred into escherichia coli BL21 (DE3) and is induced and purified to obtain the bacteriophage DNA enzyme, and the amino acid sequence of the bacteriophage DNA enzyme is shown as SEQID NO: 2. The bacteriophage DNA enzyme disclosed by the invention has relatively strong DNA degradation activity and can be applied to preparation of drugs for treating pseudomonas aeruginosa infection.

Provided herein are compositions and methods to protect cargo DNA when it is loaded into lipid nanoparticles (LNPs) or similar nanocarrier systems. In certain embodiments, the compositions include a lipid nanoparticle (LNP) that includes a DNA cargo and a molecule capable of condensing and / or compacting DNA. In certain embodiments, the compositions also include a "helper molecule" that provides reduced inflammatory response and reduced DNA degradation by DNAse enzymes and autophagy / xenophagy during and after endosomal escape, upon administration.

The present invention relates to a cosmetic composition for skin barrier improvement and skin regeneration, containing rose-derived DNA (polydeoxyribonucleotide (PDRN)) as an active ingredient. The rose-derived DNA minimizes DNA degradation caused by oxidative stress and the like, has a high molecular weight and high yield, and exhibits regeneration, wrinkle reduction, elasticity improvement and skin barrier maintenance and improvement effects superior to those of a conventional salmon-derived DNA or a low molecular weight DNA derived from other plants.

The invention belongs to the technical field of biological medicines, and particularly relates to a novel technology for preserving a DNA sample at room temperature. The technology selects calcium sulfate dihydrate as a stabilizer for DNA sample preservation, the preparation process is simple and easy to operate, the source is convenient, the cost is low, and the preservation effect is good. It is found that a DNA sample is mixed with a calcium sulfate aqueous solution, the content of DNA / CaSO4 is not higher than 25% (w / w), a solid mixture is prepared through a freeze drying technology, stable preservation of the DNA sample at the room temperature (10-40 DEG C, preferably 20-25 DEG C) can be achieved, the total loss caused by degradation of the DNA sample at the high temperature of 70-95 DEG C is reduced, the DNA degradation rate is improved, and the DNA sample can be stored stably at the room temperature (10-40 DEG C, preferably 20-25 DEG C). The STR sequence of a human genome DNA sample is stabilized; non-accelerated experiments show that the technology can maintain the effective stability of a DNA sample at room temperature for not less than 130 days.

Methods and systems of single-cell bisulfite sequencing are provided. The single-cell bisulfite sequencing techniques of the disclosure involve single-cell DNA (scDNA) droplets, where the scDNA are fragmented within the scDNA droplets prior to bisulfite conversion. The methods and systems further include barcode droplets, and fusing the scDNA droplets with the barcode droplets to form barcoded scDNA droplets for bisulfite conversion. The bisulfite conversion beneficially occurs within the bisulfite droplets to reduce DNA degradation. The single-cell bisulfite sequencing method and systems provide for bisulfite sequenced nucleic acid libraries.