Method for establishing fingerprint maps of rhaponticum uniflorum of Mongolian medicines and method for evaluating quality of rhaponticum uniflorum

Abstract

The invention relates to a method for establishing fingerprint maps of rhaponticum uniflorum of Mongolian medicines. The method includes 1), preparing test solution, to be more specific, arranging medicinal material powder in a 100 mL conical flask with a stopper, adding 70% methanol into the conical flask, carrying out microwave extraction, arranging the conical flask in a room-temperature environment, then weighing the conical flask, supplementing lost weight of the conical flask by the aid of solvents, filtering liquid to obtain filter liquid and filtering the filter liquid by the aid of filter membranes with micro-pores with the sizes of 0.45 micrometer; 2), filling an efficient liquid-phase chromatographic instrument with the test solution to obtain the fingerprint maps. Chromatographic conditions includes that chromatographic columns are octadecylsilane chemically bonded silica chromatographic columns; mobile phases include acetonitrile (A) and 0.2% phosphoric acid aqueous solution (B), and the B is eluted by 85% (for 0 min), 78% (for 12 min), 74% (for 13 min) and 60% (for 60 min) in a gradient manner. Detection conditions include that the detection wavelengths of DAD (diode array detectors) are 230-400 nm; column temperatures are 20-40 DEG C; the sample sizes are 5-30 micro-L. The invention further provides a method for evaluating the quality of the rhaponticum uniflorum of the Mongolian medicines by the aid of the fingerprint maps. The methods have the advantages that the methods are high in sensitivity and good in repeatability; the varieties and the quantities of chemical components in Mongolian medicinal materials with the rhaponticum uniflorum can be comprehensively reflected by the aid of the obtained standard fingerprint maps.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to a method for establishing fingerprints of Chinese medicinal materials and a method for evaluating the quality of medicinal materials. Background technique [0002] With the rapid development of the Mongolian medicine industry, the problem of decreasing wild resources caused by irrational use of resources and environmental changes has become prominent, seriously restricting the healthy development of the Mongolian medicine industry. Self-collection and self-use of Mongolian medicinal materials are also very common. At present, there are some local medicinal materials specially used for Mongolian medicine that are not commercially available, and some commercial medicinal materials are available in the market, but the varieties are confusing and the clinical use is unclear. Reasonable and effective use of Mongolian medicinal materials has caused great trouble. Leaking reed flower is on...

AI Technical Summary

Problems solved by technology

This not only restricts the effective utilization of the resources of the leaking

Images