Composite condensation product with double-embedding function as well as preparation method and application of composite condensation product

A complex coagulation and functional technology, applied in the field of complex coagulation and its preparation, can solve problems such as less research, and achieve the effects of rich sources, improved embedding efficiency, and enhanced biological stability

Pending Publication Date: 2021-01-12
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on protein-polysaccharide interaction at home and abroad mainly focuses on the nano-delivery system formed by water-soluble protein and water-soluble polysaccharide. Fewer, and most of the research is limited to embedding one kind of substance, there are relatively few reports on the co-embedding of substances with different polarities in the same delivery system

Method used

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  • Composite condensation product with double-embedding function as well as preparation method and application of composite condensation product
  • Composite condensation product with double-embedding function as well as preparation method and application of composite condensation product
  • Composite condensation product with double-embedding function as well as preparation method and application of composite condensation product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Preparation of 2% zein solution (Zein):

[0048] 0.2 g of zein (purchased from Sigma Aldrich Inc., USA) was dissolved in 10 ml of 80% (v / v) aqueous ethanol, stirred in a magnetic stirrer until the solution was clear, and made into 2% (20 mg / v) mL) of Zein solution.

[0049] (2) Preparation of chitosan (Cs) antisolvent solution:

[0050] The chitosan of 0.07g (molecular weight is about 300kDa; Deacetylation degree 95%) is dissolved in the acetic acid solution of 70ml 1% (v / v), stirs until solution clarification in magnetic stirrer, makes 0.1% (1mg / v) mL) of Cs anti-solvent solution, placed in a refrigerator at 4°C overnight for hydration.

[0051] (3) Preparation of ZCsNPS-Cur-Que composite condensates:

[0052] ① The ratio of fixed zein to the total weight of two polyphenols (curcumin and quercetin) is 20:1, wherein the weight ratio between curcumin (Cur) and quercetin (Que) is 1: 1. Weigh a certain amount of curcumin and quercetin and dissolve in 2% zein solut...

Embodiment 2

[0062] With reference to the preparation method in Example 1, the pH of the fixed composite particle system is 4.0, and the weight ratio between Cur and Que is 1:1, and the total amount of 2% Zein solution and two kinds of polyphenols is 20: 1. Mixing ratios of 10:1 and 5:1, that is, 0.01g (0.005g Cur and 0.005g Que), 0.02g (0.01g Cur and 0.01g Que), 0.04g (0.02g Cur and 0.02g Que ) The two total polyphenols were added to three 10mL 2% Zein solutions being stirred in a magnetic stirrer respectively, and then the Cs anti-solvent solution was quickly poured into the dissolved Zein-Cur-Cur- In the Que solution, the stirring rate was 600 rpm, and after stirring for 2 minutes, the mixed solution was transferred to a rotary evaporator. Under the conditions of 88rpm and 55°C, rotate and heat for 12min to completely evaporate the ethanol, and obtain 3 kinds of ZCsNPS-Cur-Que complex coacervates with different mixing mass ratios. The pH of the solution was adjusted to 4.0 by 1M HCl so...

Embodiment 3

[0065] With reference to the preparation method in Example 1, the pH of the fixed composite particle system is 5.0, the weight ratio between Cur and Que is 1:1, and the total amount of 2% Zein solution and two kinds of pigments (Cur and Que) is measured by mass. The ratio is 20:1, 10:1, and 5:1, that is, 0.01g (0.005g Cur and 0.005g Que), 0.02g (0.01g Cur and 0.01g Que), 0.04g (0.02g Cur and 0.02g Que) were added into 3 parts of 10mL 2% Zein solutions being stirred in a magnetic stirrer respectively, and then the Cs anti-solvent solution was quickly poured into the already dissolved solution being stirred in a magnetic stirrer In the Zein-Cur-Que solution, the stirring speed was 600rpm, and after stirring for 2min, the mixed solution was transferred to a rotary evaporator. Under the conditions of 88rpm and 55°C, rotate and heat for 12min to completely evaporate the ethanol, and obtain 3 kinds of ZCsNPS-Cur-Que complex coacervates with different mixing mass ratios. Adjust the ...

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Abstract

The invention discloses a composite condensation product with a double-embedding function as well as a preparation method and application of the composite condensation product. The preparation methodcomprises the following steps: (1) adding Zein into an ethanol water solution, and carrying out stirring for dissolving to obtain a Zein solution; (2) adding chitosan into an acetic acid solution, carrying out stirring for dissolving, and then carrying out overnight hydration to obtain a chitosan anti-solvent solution; (3) adding curcumin and quercetin into the Zein solution, carrying out uniformstirring and mixing, and then carrying out overnight hydration to obtain a Zein-Cur-Que mixed solution; and (4) adding the chitosan anti-solvent solution into the Zein-Cur-Que mixed solution, carryingout uniform stirring and mixing, then carrying out rotary heating to remove ethanol, and then adjusting the pH value to 3.0-7.0 to obtain the composite condensation product with the double-embeddingfunction. The composite condensation product disclosed by the invention can simultaneously embed two substances with different polarities, is high in embedding rate and high in bioavailability, and can be used in the field of food processing.

Description

technical field [0001] The invention belongs to the field of modern health food processing, and in particular relates to a composite coacervate with double embedding function and its preparation method and application. Background technique [0002] The use of nanoparticles as biopolymers to deliver functional ingredients such as curcumin (Cur) has aroused great interest among researchers. In particular, proteins and polysaccharides are suitable materials as nano-delivery systems due to their good biocompatibility, biodegradability, non-antigenicity, and low toxicity. Therefore, more and more scholars are committed to studying the biological significance of the system formed by the protein-polysaccharide interaction and exploring the application potential of the protein-polysaccharide complex. Complexes are formed mainly through electrostatic interactions between anionic biomacromolecules and cationic biomacromolecules, followed by hydrophobic interactions, hydrogen bonds, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/185A23L33/105A23L29/30A23L5/43
CPCA23L33/185A23L33/105A23L29/30A23L5/43A23V2002/00
Inventor 袁杨苏春儒陈琦蕙
Owner GUANGZHOU UNIVERSITY
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