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Calcium supplement of fish scale protein peptide calcium chelate and preparation method thereof

A fish scale protein and chelate technology, which is applied in the direction of peptide/protein components, anti-toxic agents, drug combinations, etc., can solve the problems of low absorption utilization rate and large side effects, and achieve lower blood pressure, increase absorption utilization rate, and safe preparation process Effect

Inactive Publication Date: 2011-09-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention uses enzymatic hydrolysis technology to prepare fish scale protein calcium chelate microcapsule calcium supplement, and provides its preparation method to overcome the defects of large side effects and low absorption and utilization rate of traditional calcium supplement products, so that the microcapsule calcium supplement can be Maintain a soluble state in the intestinal tract, so as to develop a natural, safe and efficient biological calcium supplement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 0.6kg of fish scale protein, make it into a 6% (weight to volume ratio) protein solution, add 6g of trypsin, adjust the enzymolysis temperature to 42°C, enzymolysis time is 6h, and the pH is 7.0 for enzymolysis. After the enzymolysis, 90 Inactivate the enzyme at ℃ for 10 minutes, and perform ultrafiltration with ultrafiltration equipment (the molecular weight of the ultrafiltration membrane is 3000Da) to obtain relatively high-purity fish scale protein bioactive peptides, which are cooled to room temperature for later use. The fish scale protein bioactive peptide solution, according to the bioactive peptide and CaCl 2 (as Ca 2+ A mass ratio of 5:1, a reaction pH of 6.0, a reaction temperature of 45° C., and a reaction time of 30 min were used for the chelation reaction to obtain the fish scale protein peptide calcium chelate.

[0019] The mixture of fish scale protein and polysaccharide is used as the wall material, the mass ratio of fish scale protein and polysac...

Embodiment 2

[0021] Take 0.6 kg of fish scale protein, make it into a 6% (weight to volume ratio) protein solution, add 10 g of trypsin, adjust the enzymolysis temperature to 45° C., enzymolysis time is 5 hours, and the pH is 7.2 for enzymolysis. After the enzymolysis, 70 Inactivate the enzyme at ℃ for 20 minutes, and perform ultrafiltration with ultrafiltration equipment (ultrafiltration membrane molecular weight is 3000Da) to obtain relatively high-purity fish scale bioactive peptides, which are cooled to room temperature for later use. The fish scale protein bioactive peptide solution, according to the bioactive peptide and CaCl 2 (as Ca 2+ A mass ratio of 4:1, a reaction pH of 6.3, a reaction temperature of 55° C., and a reaction time of 20 minutes were used for the chelation reaction to obtain the fish scale protein peptide calcium chelate.

[0022] The mixture of fish scale protein and polysaccharide is used as the wall material, the mass ratio of fish scale protein and polysacchari...

Embodiment 3

[0024] Take 0.6 kg of fish scale protein, make it into a 6% (weight to volume ratio) protein solution, add 15 g of trypsin, adjust the enzymolysis temperature to 45° C., enzymolysis time is 5 hours, and the pH is 7.5 for enzymolysis. After the enzymolysis, 85 Inactivate the enzyme at ℃ for 15 minutes, and perform ultrafiltration with ultrafiltration equipment (ultrafiltration membrane molecular weight is 3000Da) to obtain relatively high-purity fish scale protein bioactive peptides, which are cooled to room temperature for later use. The fish scale protein bioactive peptide solution, according to the bioactive peptide and CaCl 2 (as Ca 2+ A mass ratio of 3:1, a reaction pH of 6.5, a reaction temperature of 60° C., and a reaction time of 15 minutes were used for the chelation reaction to obtain the fish scale protein peptide calcium chelate.

[0025] The mixture of fish scale protein and polysaccharide is used as the wall material, the mass ratio of fish scale protein and poly...

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Abstract

The invention relates to fish scale protein peptide calcium chelate, a microcapsule calcium supplement of the chelate and preparation methods thereof. In the invention, calcium chloride and the enzymatic hydrolysate of fish scale protein are chelated, thus improving the solubility of the calcium salt under a subalkaline condition in the intestinal tracts and improving the absorption utilization ratio of calcium, and the preparation process is safe and pollution-free. In the invention, an appropriate wall material is also adopted for microencapsulating the fish scale protein peptide calcium chelate to prepare the microcapsule calcium supplement of the fish scale protein peptide calcium chelate; the calcium supplement can cover up the off flavor of the calcium salt and can gradually release Ca<2+> under the subalkaline condition in the intestinal tracts, and the biological absorption utilization ratio of the Ca<2+> is high without toxic side effect; and due to the fish scale protein peptide, the fish scale protein peptide calcium chelate not only can prevent and treat osteoporosis, but also has the physiological functions of resisting oxidation, adjusting immunity, reducing blood pressure and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fish scale protein peptide calcium chelate, its microcapsules, their preparation method and biological application. Background technique [0002] Calcium is one of the essential mineral elements for the human body. Calcium is an important component of bones and teeth. Calcium participates in the stress process of nerve and muscle tissue, and also participates in blood coagulation, regulation of enzyme activity, hormone secretion, and maintenance of acid-base balance of body fluids. And regulate the normal physiological function of cells. However, ingredients such as phosphate and oxalic acid in food interfere with the absorption of calcium, and the absorption and utilization rates of inorganic calcium supplement products and organic calcium supplement products are also low. Therefore, finding a safe and effective calcium supplement product is of great significance to h...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K38/39A61K9/50A61P3/02A61P39/06A61P37/02A61P9/12
Inventor 刘尊英黄海吴浩浩
Owner OCEAN UNIV OF CHINA
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