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Preparation method of collagen polypeptide chelated calcium

A technology of collagen polypeptide and chelated calcium, which is applied in the field of synthesis of food additives and calcium supplements, can solve the problem of less calcium chelated by collagen polypeptide, and achieve the effects of increasing added value, reducing pollution, and being easy for human body to absorb

Active Publication Date: 2012-01-11
SHANGHAI FOOD RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the calcium supplement preparations using amino acid chelated calcium as raw material have not been fully developed in China, and collagen polypeptide chelated calcium is even less

Method used

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  • Preparation method of collagen polypeptide chelated calcium
  • Preparation method of collagen polypeptide chelated calcium
  • Preparation method of collagen polypeptide chelated calcium

Examples

Experimental program
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Effect test

Embodiment 1

[0028] (1) Select 100g of food-grade pigskin gelatin, gelatin to water mass ratio of 1:8, swell for 30 minutes, dissolve in a boiling water bath, adjust temperature to 50°C, pH 5.0, add compound protease (Protamex) 1.5 according to the enzyme-material ratio of 1.5% g, enzymatic hydrolysis for 3 hours; after inactivating the enzyme in a boiling water bath for 10 minutes, adjust the temperature to 55°C, pH 6.0, and the ratio of enzyme to material to 5 LAPU / g, add flavor protease (Flavourzyme), enzymolysis for 3 hours, inactivate the enzyme in a boiling water bath for 10 minutes, and obtain small Molecular weight collagen peptide solution;

[0029] (2) Add food-grade calcium chloride to the small molecule collagen polypeptide obtained above for chelation. The chelation conditions are: temperature 20°C, pH 6.0, adding ratio of 110mg calcium ion / g collagen polypeptide, water bath chelation 30min, after the chelation is completed, precipitate with absolute ethanol, centrifuge to rem...

Embodiment 2

[0033] (1) Choose food grade fish skin gelatin 100g, gelatin and water mass ratio is 1: 10 swelling 30min, after boiling water bath dissolves, adjust temperature 50 ℃, pH 6.0, add compound protease (Protamex) 1.5 according to enzyme material ratio is 1.5% g, enzymatic hydrolysis for 3 hours, after inactivating the enzyme in a boiling water bath for 10 minutes, adjust the temperature to 55°C, pH 6.0, and the ratio of enzyme to material to 10 LAPU / g, add flavor protease (Flavourzyme), enzymolysis for 3 hours, inactivate the enzyme in a boiling water bath for 10 minutes, and obtain small Molecular weight collagen peptide solution;

[0034] (2) Add food-grade calcium lactate to the small molecule collagen polypeptide obtained above for chelation. The chelation conditions are: temperature 30°C, pH 6.0, adding ratio of 130mg calcium ion / g collagen polypeptide, chelation in water bath for 30min After the chelation is completed, precipitate with absolute ethanol, centrifuge to remove ...

Embodiment 3

[0037] (1) Choose 100g of pharmaceutical grade cowhide gelatin, the gelatin to water mass ratio is 1:12, after swelling for 30min and dissolving in a boiling water bath, adjust the temperature to 40°C, pH 7.0, add compound protease (Protamex) 0.5 according to the enzyme material ratio of 0.5% g, enzymatically hydrolyze for 6 hours, inactivate the enzyme in a boiling water bath for 10 minutes, adjust the temperature to 60°C, pH 7.0, according to the enzyme material ratio of 20LAPU / g, add flavor protease (Flavourzyme), enzymolyze for 4 hours, inactivate the enzyme in a boiling water bath for 10 minutes, and obtain Collagen peptide liquid with small molecular weight;

[0038] (2) Add food-grade calcium gluconate to the small molecule collagen polypeptide obtained above for chelation. The chelation conditions are: temperature 50°C, pH 5.0, adding ratio of 70mg calcium ions / g collagen polypeptide, water bath chelation After 10 minutes, after the chelation is completed, precipitate ...

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Abstract

The invention provides a preparation method of collagen polypeptide chelated calcium. The preparation method comprises the following steps of: performing enzymolysis on animal skin gelatin by a two-step enzymolysis method to prepare micromolecule collagen polypeptide abundant in oxyproline; and performing chelation reaction on the micromolecule collagen polypeptide and food-grade soluble ionized calcium to prepare the collagen polypeptide chelated calcium. The process for preparing the collagen polypeptide chelated calcium is simple; the chelation rate reaches 77 milligrams of ionized calciumper gram of collagen polypeptide; the prepared collagen polypeptide chelated calcium has high water solubility, is absorbed by a human body easily, supplements the calcium element required by the human body, also supplements the oxyproline for promoting calcium absorption, and is a good calcium supplement product.

Description

technical field [0001] The invention relates to a preparation method of collagen polypeptide chelated calcium, which belongs to the technical field of synthesis of food additives and calcium supplements. Background technique [0002] Calcium is one of the most abundant mineral elements in the human body. Among all the elements that make up the human body, it ranks fifth after carbon, hydrogen, oxygen, and nitrogen. Calcium is an important component of bones and teeth, an element necessary for animal life activities, and the source of animal body metabolism. Animal life activities such as transmission of nerve substances, heartbeat, muscle contraction, etc. are all using and consuming calcium. . Due to many reasons such as eating habits, living habits, age levels, etc., calcium is still one of the most deficient nutrients in the Chinese population. With the improvement of people's living standards and the enhancement of health awareness, people are increasingly aware of th...

Claims

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Application Information

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IPC IPC(8): A23L1/304A23L1/305
Inventor 马志英沈亚领沈菊泉严维凌谢志镭彭巧云孙佳奇陈平任莉萍杨琦林露
Owner SHANGHAI FOOD RES INST
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