41 results about "Mouse Hippocampus" patented technology

The invention relates to application of ginseng pectin in resisting depressive behavior, and belongs to the field of traditional Chinese medicine application. Ginseng pectin is given to a mouse for 12 days in force feeding manner, and the activity of resisting a depressive behavior is evaluated by an open-field locomotor activity test, an elevated plus maze test, a social interference test and a forced swimming test. Results indicate that by using the ginseng pectin, the interference time and frequency in a mouse social interference test can be increased, the invasion time and frequency of the mouse can be reduced, the time rate and frequency rate of an open-arm of the mouse entering an elevated plus maze can be increased, the standing time of the mouse forced swimming test can be reduced, and protein expression of hippocampus beta-catenin and brain-derived neurotrophic factor (BDNF) of the mouse can be increased along with changes of the behaviors, namely the ginseng pectin can be used for enhancing release of transmitters among synapses and connection among synapses by inducing the protein expression of hippocampus beta-catenin and BDNF so as to influence the neuron plasticity and improve neuron development and achieve the activity of resisting the depressive behavior.

The invention belongs to the field of medicine, and discloses new applications of triptorelin, especially applications of triptorelin in preparation of drugs for inhibiting neuroinflammation. According to the present invention, the experiment results show that triptorelin can inhibit microglia activation, can significantly inhibit LPS-induced inflammatory mediator (TNF-alpha and iNOS) release in mouse hippocampus, and can significantly increase the resolution of new and old things in LPS-induced learning and memory impairment mice, wherein the spontaneous alternation rate in Y maze is increased significantly, such that it is indicated that triptorelin has significant protection effects on LPS-induced learning and memory impairment in mice; and triptorelin has remarkable neuroinflammation inhibition activity, can be used for preparing drugs for preventing and / or treating neuroinflammation, acute and chronic neurodegenerative diseases and other related diseases, and has high clinical application value and development prospect.

The invention provides a construction method and application of an AAV virus vector capable of specifically expressing Cre recombinase in a hippocampus CA2 region of a mouse. The construction method comprises following steps: a partial promoter region of a mouse hippocampus CA2 specific expression gene Map3k15 is intercepted to be connected with Cre recombinase and cloned to a pAAV vector withoutan initial promoter. The constructed AAV vector with the Map3k15 promoter connected with Cre recombinase can effectively realize specific overexpression of Cre recombinase in the hippocampus CA2 region of a mouse so as to realize specific regulation and control of neuron activity in the CA2 region; a CA2 neuron tracing and regulating system independent of Cre transgenic mice is established, an effective tool is provided for researching functions of the CA2 region, and a basis is provided for subsequent research on CA2 functional molecular mechanism.

The invention relates to new application of mulberroside A and its derivative resveratrol in preparation of a drug for protecting the intestinal barrier. In the drug, the mulberroside A or the derivative thereof is an active ingredient. The influence of mulberroside A and its derivative on hippocampus neuroinflammation of high-fructose diet mice, the influence on intestinal barrier damage of high-fructose diet mice and the influence on intestinal flora disorder of high-fructose diet mice are studied through pharmacological experiments. It is found that the mulberroside A and its derivative canobviously inhibit activation of central microglial cells and inflammatory factor level of mice with high fructose diet. It is proved that the mulberroside A and its derivative have the effect of protecting the intestinal barrier, can be used for preparing a drug for preventing and / or treating central nervous inflammation and related neurodegenerative diseases caused by intestinal barrier damage,can remodel intestinal flora disorder caused by high fructose diet and are used for preparing a drug for adjusting intestinal flora disorder.

The invention discloses a traditional Chinese medicine composition, a preparation method and application thereof. The traditional Chinese medicine composition is composed of Coptidis Rhizoma Cortex Phellodendron, Cortex Phellodendri, Gardenia, Salvia Miltiorrhiza, Curcuma longa and Shichangpu. The traditional Chinese medicine composition can significantly improve the learning and memory dysfunction of 5×FAD transgenic mice, reduce the expression of Aβ40 / 42 in the hippocampus, and increase the 5 The expression levels of pre- and post-synaptic proteins in the hippocampus of ×FAD transgenic mice can improve the excitatory synaptic transmission disorder in the hippocampus of 5×FAD mice, and at the same time inhibit the apoptosis of hippocampal neurons and the proliferation of microglia in 5×FAD mice In addition, it can inhibit the expression of inflammatory factors in the serum and hippocampus of 5×FAD mice, which provides a reliable theoretical basis for the preparation of drugs for the treatment or adjuvant treatment of Alzheimer's disease.

The invention relates to the technical field of biomedicine technology, and particularly discloses application of inhibitors of miRNA-182, miRNA-188 and miRNA-199a. The therapeutic effect of a miRNA inhibitor AM-182 / 188 / 199a on AD is clarified, specifically, the neuronal function of the hippocampus of AD mice and the plasticity of neuronal neurites are improved, the formation of A[beta] is reduced, the learning and memory ability of the AD mice are improved, new prevention and treatment methods are provided for the clinical treatment of AD, and new ideas are provided for the preparation of drugs for the treatment of AD.

The invention discloses an application of dioscin to preparation of medicines for treating Alzheimer's diseases. The Morris water maze testing result shows that a dioscin administration group can shorten the escaping latency period, prolong original platform quadrant residence time and increase the number of times of passing through a platform, which indicates that the dioscin can significantly improve the spatial learning and memorizing capacity of AD mice; the gait analysis result indicates that the dioscin can significantly increase the gait indexes of the AD mice, and strengthen the physical strength of the AD mice, so that the movement velocity of the AD mice is accelerated, the stride frequency is increased, the stepping length is increased and the duty cycle can be significantly shortened; the dioscin can significantly reduce the MDA level in hippocampal tissue of the AD mice, increase the GSH level and the SOD level, and improve oxidative damage of brain tissue of the AD mice;and the dioscin can restrain AchE activity of the hippocampal tissue, increase ChAT level, influence a cholinergic system, obviously improve the pathology structure of the brain tissue of the AD miceand ultrastructural damage of nerve cells. The dioscin can be used as a single effective component to be prepared into medicines, and can be combined with other medicines for preparing a compound preparation.

The invention belongs to the field of medicine, and discloses new applications of triptorelin, especially applications of triptorelin in preparation of drugs for inhibiting neuroinflammation. According to the present invention, the experiment results show that triptorelin can inhibit microglia activation, can significantly inhibit LPS-induced inflammatory mediator (TNF-alpha and iNOS) release in mouse hippocampus, and can significantly increase the resolution of new and old things in LPS-induced learning and memory impairment mice, wherein the spontaneous alternation rate in Y maze is increased significantly, such that it is indicated that triptorelin has significant protection effects on LPS-induced learning and memory impairment in mice; and triptorelin has remarkable neuroinflammation inhibition activity, can be used for preparing drugs for preventing and / or treating neuroinflammation, acute and chronic neurodegenerative diseases and other related diseases, and has high clinical application value and development prospect.

The invention discloses an application of Cistanche deserticola polysaccharide in medicine preparation used for improving learning memory function, and is characterized in that a channel water labyrinth and a Morris labyrinth are used for respectively discussing the influences of the mouse learning memory acquired obstacle caused by Cistanche deserticola polysaccharide on the scopolamine hydrobromide, the mouse learning memory reproducibility obstacle caused by ethanol and the mouse learning memory consolidation obstacle, which show that the Cistanche deserticola polysaccharide has intelligence promotion effect, and the application provided by the invention makes a research for a effect mechanism that the Cistanche deserticola polysaccharide improves the learning memory function to reduce the mouse hippocampus and cerebral cortex AChE active content and increase the content of Ach in brain.

The invention relates to an application of chitosan nanogel loaded with oxytocin in preparation of a medicine for early intervention of Alzheimer's disease. The research finds that the chitosan nanogel loaded with oxytocin has a remarkable effect in the aspect of early treatment of the Alzheimer's disease. Cellular level experiment results show that the chitosan nanogel loaded with the oxytocin can inhibit microglial cell activation caused by lipopolysaccharide and reduce expression of inflammatory factors in microglial cells. Animal level experiment results show that the chitosan nanogel loaded with the oxytocin can significantly reduce the expression level of inflammatory factors of hippocampus of an early model mouse with the Alzheimer's disease, significantly reduce beta-amyloid protein deposition and neuron damage, and significantly improve the learning and memory ability of the early model mouse with the Alzheimer's disease. The chitosan nanogel loaded with the oxytocin is simple in preparation process, mild in reaction condition and easy in surface modification.

The invention provides a traditional Chinese medicine composition for improving memory and mild cognitive impairment. The traditional Chinese medicine composition comprises 40-80 parts of thinleaf milkwort root-bark, 40-80 parts of grass leaf sweetflag rhizome, 40-80 parts of ginseng, 80-120 parts of tall gastrodia tuber, 80-120 parts of papermulberry fruit, 80-120 parts of ginkgo leaves and 0.01-3 parts of borneol. The traditional Chinese medicine composition can achieve the effects of obviously increasing the number density and surface density of synapse of the molecular layer of mouse hippocampal CA1, obviously enhancing the SOD activities of central cerebral cortex and hippocampus, reducing the content of lipofuscin of the neuron, obviously improving the learning and memory impairment of spontaneous senile dementia model mice, obviously enhancing the activity and fiber density of NOS1 of the central cerebral cortex and hippocampus and obviously up-regulating the contents of SS, AVP and ACTH of the cerebral cortex and hippocampus, especially the content of SS. The traditional Chinese medicine composition can achieve the effect of obviously improving the clinical symptoms of memory and cognitive impairment and has definite curative effects and great application prospects.

The invention provides a construction method and application of an AAV virus vector capable of specifically expressing Cre recombinase in the CA2 region of mouse hippocampus. The invention cuts out the partial promoter region of mouse hippocampus CA2 specific expression gene Map3k15 and connects it with Cre recombinase, and clones it into the pAAV vector without initial promoter. The AAV vector with the Map3k15 promoter linked to the Cre recombinase constructed by the present invention can effectively specifically overexpress the Cre recombinase in the CA2 region of the mouse hippocampus to achieve specific regulation of neuron activity in the CA2 region, and establishes a small gene that does not depend on the Cre transgene. The mouse CA2 neuron tracing and regulation system provides an effective tool for studying the function of the CA2 region, and provides a basis for the subsequent study of the molecular mechanism of CA2 function.

The application belongs to the field of medicine, and discloses the application of triptorelin, especially the application of triptorelin in the preparation of medicaments for treating depression. Experiments have shown that in the forced swimming test, triptorelin can significantly reduce the immobility time of depression model mice induced by chronic restrictive stress, lipopolysaccharide, plantar injection of CFA and early social isolation, indicating that it has a positive effect on depression model mice. Obvious improvement. At the same time, triptorelin can significantly increase the expression of chronic restrictive stress-induced mouse hippocampal BDNF and neuronal synaptic plasticity-related genes c-fos, egr1, arc, npas4. Therefore, triptorelin has significant antidepressant activity, can be used to prepare medicines for treating depression, and has high clinical application value and development prospect.

The invention discloses an auxiliary positioning device for mouse brain injection, which comprises a constant temperature base plate, a vertical axis moving scale and a clamping assembly. The four corners of the lower surface of the constant temperature base plate are threaded with non-slip feet, and the inside of the constant temperature base plate is installed with A constant temperature heating assembly, a fixed component is fixed on the rear side of the upper surface of the constant temperature bottom plate, the vertical axis moving scale is installed on the left surface of the constant temperature bottom plate, and the upper end of the vertical axis moving scale is fixed with a vertical plate, the inside of the vertical axis A screw is installed in the middle position, and the top of the screw is welded with an adjustment handle. The outer wall of the screw is threaded with a ball slide, and the right side of the ball slide is equipped with a horizontal axis moving scale, and the clamping assembly is installed on the horizontal axis. Move the ruler below. The present invention greatly improves the accuracy of the entire positioning device by adopting a moving scale with a positioning accuracy of 0.01mm, thus solving the problem in the prior art that precise positioning cannot be performed due to the small hippocampus of mice.