Method for extracting isoflavone from kudzuvine root efficiently

A technology for isoflavones and pueraria, which is applied in the field of efficient extraction and preparation of isoflavones, can solve the problems of low extraction rate, low efficiency, water extraction purity, and low extraction rate, and achieves the effects of improving dissolution rate and extraction rate and reducing cost.

Inactive Publication Date: 2012-01-18
JIANSU MAOBAO GEYE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The purity obtained by alcohol extraction is high, but the process is cumbersome, the loss of active ingredients is large, the extraction rate is low, the time is long and the cost is high, and it is mainly used for medical and clinical purposes
The water extraction method has the disadvantages of low purity and extraction rate
[0003] In view of the disadvantages of high energy consumption, long time and low efficiency in the traditional extraction methods of kudzu root isoflavones such as heating reflux method, percolation method and dipping method, it is not suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Get Jurong wild kudzu root 1000g and pulverize to 60-80 purpose kudzu root powder. Add Pueraria root powder to 10L of 70% ethanol solution for extraction, adjust the pH to 6, add 100g cellulase and 100g protease at 60°C for enzymolysis, and the enzymolysis time is 60min to obtain Pueraria root extract. After enzymolysis, the Pueraria root extract was treated by ultrasonic-microwave treatment, the ultrasonic frequency of the ultrasonic-microwave treatment was 40KHz, the output power of the ultrasonic transducer was 40KHz, the microwave power was 86-96W, and the temperature was controlled at 45-53°C for 25 minutes. Then concentrated under reduced pressure and freeze-dried to obtain kudzu root isoflavones.

Embodiment 2

[0016] Get Jurong wild kudzu root 1000g and pulverize to 60-80 purpose kudzu root powder. Add Pueraria root powder to 25L of 70% ethanol solution for extraction, adjust the pH to 6, add 125g cellulase and 250g protease at 60°C for enzymolysis, and the enzymolysis time is 66min to obtain Pueraria root extract. After enzymolysis, the Pueraria root extract was treated by ultrasonic-microwave treatment, the ultrasonic frequency of the ultrasonic-microwave treatment was 40KHz, the output power of the ultrasonic transducer was 40KHz, the microwave power was 86-96W, and the temperature was controlled at 45-53°C for 30 minutes. Then concentrated under reduced pressure and freeze-dried to obtain kudzu root isoflavones.

Embodiment 3

[0018] Get Jurong wild kudzu root 1000g and pulverize to 60-80 purpose kudzu root powder. Add Pueraria root powder to 35L of 70% ethanol solution for extraction, adjust the pH to 6, add 88g of cellulase and 262g of protease at 60°C for enzymolysis, and the enzymolysis time is 58min to obtain Pueraria root extract. After enzymolysis, the Pueraria root extract was treated by ultrasonic-microwave treatment, the ultrasonic frequency of ultrasonic-microwave treatment was 40KHz, the output power of the ultrasonic transducer was 40KHz, the microwave power was 86-96W, and the temperature was controlled at 45-53°C for 20 minutes. Then concentrated under reduced pressure and freeze-dried to obtain kudzu root isoflavones.

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Abstract

The invention discloses a method for extracting isoflavone from kudzuvine root efficiently, and belongs to the technical field of the processing of agricultural products and food. The method comprises the following steps of: (1) crushing, namely crushing kudzuvine root to form 60 to 80-mesh kudzuvine root powder; (2) performing enzymolysis, namely adding 70 percent of ethanol into the kudzuvine root powder, adding cellulase and protease and performing the enzymolysis to obtain kudzuvine root extract, wherein 1 to 2 grams of the cellulase and the protease are added into each 100 milliliters of solution; and (3) extracting, namely performing ultrasonic-microwave processing on the obtained kudzuvine root extract from the step (2), concentrating under reduced pressure, freezing and drying to obtain kudzuvine root isoflavone. In the method, the kudzuvine root isoflavone is extracted by a compound enzymolysis technology of the cellulase and the protease and combining an ultrasonic-microwave assisted extraction technology, so the technical advantages of chemical enzymolysis and physical extraction are expressed fully, the cost is reduced, and the dissolution rate and extraction rate of the kudzuvine root isoflavone are improved, so that the extraction purity is greatly higher than that by conventional extraction method.

Description

technical field [0001] The invention relates to a method for extracting compounds from plants, in particular to a method for efficiently extracting and preparing isoflavones from kudzu root. Background technique [0002] In recent years, the extraction and efficacy of isoflavones have attracted much attention. Currently, the methods for extracting pueraria isoflavones from Pueraria radiata are: heating reflux method, percolation method, dipping method, physical enhanced assisted extraction, etc., and the extraction rate of active ingredients in reflux method is lower than High, but the amount of solvent is large, the cost is high, and the waste liquid pollutes the environment; the percolation method uses less solvent, but the extraction time is long. At present, the extraction of kudzu root total isoflavones generally adopts hot extraction method in production, but it has the disadvantages of high energy consumption, long time and low efficiency. Pueraria flavonoids are usu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/488A61K125/00
Inventor 夏荣光陈义勇赵晓联李书娟翁夕锋
Owner JIANSU MAOBAO GEYE CO LTD
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