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Preparation and electrochemical sensing application research of tin dioxide-three-dimensional graphene nanocomposite immobilized protein modified electrode

A nanocomposite material and protein modification technology, which is applied in the direction of material electrochemical variables, material analysis, material analysis by electromagnetic means, etc., can solve the difficult problems of direct electrochemistry, shedding or denaturation, and achieve low preparation cost and detection The effect of low limit and wide detection range

Pending Publication Date: 2018-04-20
HAINAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the active centers of enzymes are usually buried deeply, it is difficult to perform direct electrochemistry on the surface of the electrode, and they are directly adsorbed on the surface of the bare electrode and are prone to shedding or denaturation.

Method used

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  • Preparation and electrochemical sensing application research of tin dioxide-three-dimensional graphene nanocomposite immobilized protein modified electrode
  • Preparation and electrochemical sensing application research of tin dioxide-three-dimensional graphene nanocomposite immobilized protein modified electrode
  • Preparation and electrochemical sensing application research of tin dioxide-three-dimensional graphene nanocomposite immobilized protein modified electrode

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Experimental program
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Effect test

Embodiment 1

[0024] Preparation of ionic liquid carbon paste electrode: Weigh a certain amount of graphite powder and N-hexylpyridine hexafluorophosphate (HPPF 6 ) and add liquid paraffin as a binder, put it into a mortar and grind it thoroughly. After being fully ground, fill it into a glass electrode tube with an inner diameter of 4 mm, insert a copper wire as a wire, and compact the solid mixture in the glass electrode tube to obtain CILE. Polish the electrode surface to a mirror surface before use.

Embodiment 2

[0026] Preparation of SnO 2 -3DGR: Take 10 mL of 3.6 mg / mL graphene oxide (GO) aqueous solution and sonicate for 30 min, and weigh 0.066 g SnCl 2 • 2H 2 O and 0.054 g urea were added to 20 mL and stirred until fully dissolved, then added dropwise to GO and stirred rapidly for 1 h, then transferred to a 50 mL autoclave and heated at 180 °C for 16 h, cooled to room temperature to obtain a cylindrical hydrogel gel, washed three times with water and ethanol, and then freeze-dried at -87°C for 12 h to obtain SnO 2 -3DGR nanocomposite, the SnO 2 -3DGR electron microscope characterization picture figure 1 As shown, it can be seen that the loaded SnO 2 The size of the nanoparticles is about 4 nm.

Embodiment 3

[0028] Preparation of CTS / Mb / SnO 2 -3DGR / CILE composite film modified electrode: the prepared SnO 2 -3DGR suspension was dropped onto the surface of CILE to obtain SnO 2 -3DGR modified electrode, after drying, add the prepared Mb dropwise, after natural drying, add CTS dropwise to obtain CTS / Mb / SnO 2- 3DGR / CILE ionic liquid carbon paste electrode.

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Abstract

The invention discloses preparation and electrochemical sensing application research of a tin dioxide-three-dimensional graphene (SnO2-3DGR) nanocomposite immobilized protein modified electrode, and belongs to the technical field of biosensors. A method provided by the invention comprises the following steps: preparing a SnO2-3DGR nanometer material, dispensing the SnO2-3DGR nanometer material onto the surface of a carbon ionic liquid electrode (CILE), then dispensing myoglobin (Mb) onto the SnO2-3DGR modified CILE surface, naturally airing, dropwise adding a chitosan (CTS) solution, and drying a third-generation electrochemical enzyme sensor. The prepared sensor has the characteristics of a simple process, convenient operation, low cost, high detection sensitivity, strong electrical signal and simple electrode pretreatment, and can be used for detecting organic small molecular trichloroacetic acid (TCA), with a detection limit of 1.67 mmol / L.

Description

technical field [0001] The invention relates to a SnO2-3DGR nano composite material immobilized protein modified ionic liquid carbon paste electrode; the invention also relates to a preparation method of the modified electrode and its application in electrochemical sensing and detection. Background technique [0002] Carbon paste electrode is a kind of carbon electrode made by mixing conductive graphite powder and hydrophobic binder into a paste, and then filling it in the electrode tube. It has the advantages of simple preparation, low price and good selectivity. , high sensitivity, wide potential application range and easy surface renewal. Room temperature ionic liquids refer to liquid substances composed entirely of anions and cations at room temperature and nearby temperatures, which have the advantages of wide electrochemical window, high conductivity, good thermal and chemical stability, etc. Ionic liquid is used as a hydrophobic binder and graphite powder to prepare ...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30G01N27/327
CPCG01N27/308G01N27/3278G01N27/48
Inventor 孙伟文作瑞牛学良李晓燕赵文舒李小宝
Owner HAINAN NORMAL UNIVERSITY
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