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Molecular marker closely linked to Chinese cabbage turnip mosaic virus resistance gene retrcs03 and application thereof

A technology of turnip mosaic virus and molecular marker, which is applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of low linkage degree of molecular markers, and achieves a simple, fast and stable screening method. High, low-dose effect

Active Publication Date: 2019-10-25
VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Previous studies only initially screened three molecular markers BrID90143 (4.2cM), BrSSR4068 (4.2cM) and BrID10645 (10.1cM) linked to this gene (Zeng Qiang et al. 2014), but the linkage degree of the above molecular markers is not high

Method used

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  • Molecular marker closely linked to Chinese cabbage turnip mosaic virus resistance gene retrcs03 and application thereof
  • Molecular marker closely linked to Chinese cabbage turnip mosaic virus resistance gene retrcs03 and application thereof
  • Molecular marker closely linked to Chinese cabbage turnip mosaic virus resistance gene retrcs03 and application thereof

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Embodiment 1

[0070] Example 1 Screening of Molecular Markers Closely Linked to Gene retrcs03, Marker Sequencing and Gene Mapping

[0071] Plant material: The disease-resistant parent material used in the present invention is the high-generation inbred line material '73' of Chinese cabbage with high resistance to TuMV, the susceptible material is '06-247', and the segregation population is '06-247'×'73' ×'73' backcross population. The above-mentioned materials were sterilized with the seedling substrate imported from Danish Pinshi, then sowed in 6.5cm plastic seedling pots, and placed in an artificial climate room for cultivation. The culture conditions are: temperature 25°C, humidity 60%, light intensity 8000-9000 lux.

[0072] Toxic source material: C4 strain of TuMV, cited from Vegetable and Flower Research Institute of Chinese Academy of Agricultural Sciences, multiplied and cultivated on susceptible materials one month before use.

[0073] Identification of TuMV inoculation: When the...

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Abstract

The invention provides a molecular marker closely linked to a Chinese cabbage turnip mosaic virus resistance gene retrcs03 and application thereof, and belongs to the field of biotechnology. The molecular marker further locates a Chinese cabbage TuMV resistance gene retrcs03, contributes to better utilization in Chinese cabbage anti-TuMV molecular marker-assisted selection, meanwhile can locate the gene, and lays a foundation for the cloning, further utilization and research of the gene. At the same time, the application of the molecular marker in breeding work can greatly reduce the economicloss caused by TuMV epidemic to Chinese cabbage production, is beneficial to reducing production cost, and has great application potential and high economic value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker closely linked with Chinese cabbage turnip mosaic virus resistance gene retrcs03 and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Turnip mosaic virus (TuMV for short) belongs to the family Potyviridae and the genus Potyvirus Y, and is mainly transmitted through contact with aphids or sap. TuMV is the virus with the widest host range and the most harmfulness in the family Potatoviridae. It is widely distributed in the world. It is distributed in all continents except Antarctica, and the host range is very wide. It can infect 43 fam...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 李巧云满卫萍赵智中张志刚刘栓桃王荣花
Owner VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI
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