Bivalent carrier of against disease for coat protein gene of turnip mosaic virus and coat protein gene of cucumber mosaic virus

Abstract

This invention provides a Bivalent carrier of against disease for coat protein gene of turnip mosaic virus and coat protein gene of cucumber mosaic virus. This carrier of pCam-TuMVCP-CMVCP consists of hygromycin, NoS terminator NoSter, Cucumber Mosaic virus Coat protein gene CMVCP, 35S promoter CaMV35Spro, Turnip Mosaic Virus Coat protein gene TuMVCP, and they connects each other one by one. TuMVCP and CMVCP is constructed on the same express carrier of plant. Because of CP has stopped uncoating process of the virus gene sequence. When the bare nucleotide of the inbreak virus enters into the cell, it is then re-covered by the free CP, and then the carrier stops the duplicate of the nucleotide; CP counterworks the virus particulate to expand and, then enhance the anti-disease ability of plants.

Description

technical field [0001] The invention relates to a bivalent disease-resistant vector pCam-TuMVCP-CMVCP of a turnip mosaic virus coat protein gene and a cucumber mosaic virus coat protein gene. Belongs to the field of biotechnology. Background technique [0002] Cruciferous vegetables are an important vegetable crop, and viral diseases are very common and extremely harmful diseases in the vegetable production process. For example, in the vegetable production areas of Zhejiang, the production reduction due to viral diseases can reach 30%-40% in general years, and the production reduction can reach more than 80% in severe years, or even no harvest. The period from the seedling stage to before and after the expansion of the head of the vegetable is a period of serious disease, which often causes the growth of the plant to weaken and even cause death. Although there are slight differences in the pathogens reported in various places, the pathogens of viral diseases are mainly tur...

AI Technical Summary

Problems solved by technology

Usually the prevention and treatment of vegetable virus diseases is to spray pesticides to prevent the spread of viruses, but the control effect is not ideal, and it will pollute the environment

Weak virus strains are hard to find by taking advantage of cross-protection

Traditional breeding methods to cultivate plant varieties resistant to viral diseases will encounter problems such as that the disease resistance of plants is often closely linked to undesirable traits, or the resistance is controlled by multiple genes, making the virus resistance unsatisfactory

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