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Application of arabidopsis thaliana AtSR33 gene in inhibition of turnip mosaic virus infection

A technology of turnip mosaic virus and Arabidopsis thaliana, applied in the field of genetic engineering, can solve problems such as insufficient resistance, dwarfing of plants, and production failure, and achieve the effect of improving disease resistance and hindering infection

Active Publication Date: 2022-01-04
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

TuMV seriously harms the growth of vegetables, causing plant dwarfing, yellow wilt, mosaic, etc., and can cause serious yield loss or even extinction on cabbage
In addition, TuMV can also be spread by rubbing inoculation with aphids and sap leaves. In addition, there are different strains of TuMV in the field, which brings great difficulties to the control
[0003] At present, there are no pesticides that can effectively inhibit plant viruses. The most important thing in the prevention and control of plant viruses is the selection of disease-resistant varieties. Some anti-virus genes have been screened out in plants, but most of them have insufficient resistance. Therefore, finding genes with obvious anti-virus effects is particularly important in disease-resistant breeding

Method used

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  • Application of arabidopsis thaliana AtSR33 gene in inhibition of turnip mosaic virus infection
  • Application of arabidopsis thaliana AtSR33 gene in inhibition of turnip mosaic virus infection
  • Application of arabidopsis thaliana AtSR33 gene in inhibition of turnip mosaic virus infection

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Embodiment Construction

[0044]The following experimental steps are exemplary, aiming to provide further instructions on the construction of AtSR33-CFP overexpression vector and the application of Arabidopsis overexpression materials to inhibit TuMV infection. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0045] After in-depth analysis of the functions of SR subfamily proteins, the Col-0 Arabidopsis AtSR33 gene was cloned by PCR method; the nucleic acid sequence of the gene is shown in SEQ ID NO: 1, and the amino acid sequence is shown in SEQ ID NO: 2.

[0046] (1) Cloning the AtSR33 gene in Arabidopsis

[0047] ① Extract the total RNA of Arabidopsis thaliana Col-0 wild type, and reverse transcribe it into cDNA and use the cDNA as a template, use the FastPfu DNA Polymerase (full type gold) polymerase of Quanshijin Company, and use gw-AtSR33-F , gw-AtSR33-R i...

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Abstract

The invention discloses an application of an arabidopsis thaliana AtSR33 gene in inhibition of turnip mosaic virus infection. A constructed infectious cloning vector pEarleygate 102-AtSR33-CFP-HA is used, and the construction method of the vector comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification on an arabidopsis thaliana AtSR33 gene through a gateway system, connecting to a pDONR221 vector through a BP (Back Propagation) reaction, and connecting to a pEarleygate 102 vector through an LR (Local Regulation) reaction, so as to obtain the overexpression vector containing the AtSR33. The arabidopsis thaliana plant capable of stably expressing AtSR33 is obtained in a genetic transformation mode, and experiments show that the arabidopsis thaliana AtSR33 overexpression plant can remarkably inhibit infection of turnip mosaic viruses.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the application of Arabidopsis thaliana AtSR33 gene in inhibiting the infection of turnip mosaic virus and its infectious cloning vector. Background technique [0002] Turnip mosaic virus (TuMV), belonging to the family Potatoviridae and the genus Potatovirus, is one of the most important plant viruses in agricultural production. It is known that TuMV can infect at least 318 species of plants in 156 genera of 43 families, and is harmful all over the world. TuMV is distributed in most vegetable growing areas in my country. On vegetable crops, TuMV can harm Cucurbitaceae, Solanaceae, leguminous vegetables, the most important damage to cruciferous vegetables, including rapeseed, cabbage, cabbage, etc., has become an important pathogen that endangers cruciferous vegetables in my country. Other new hosts for TuMV were also discovered. TuMV seriously harms the growth of v...

Claims

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Application Information

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IPC IPC(8): C12N15/84C12N15/65C12N15/62C12N15/29A01H5/00A01H6/20
CPCC12N15/8205C12N15/8283C12N15/8212C07K14/415C07K2319/60
Inventor 李方方周雪平赵斯文
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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