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Cultivation and application methods of arabidopsis xpo1a/xpo1b+- double mutant

A technology of m-xpo1a-rp and m-xpo1b-rp, applied in the field of Arabidopsis thaliana cultivation, can solve the problems of restricting the use of anti-TuMV varieties, long cycle, low efficiency, etc., and is beneficial to subsequent scientific research and field application, genetic The effect of clear background and stable antiviral properties

Active Publication Date: 2019-08-23
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Problems solved by technology

At present, the prevention and control of TuMV mainly relies on resistant varieties, but relying on finding resistant varieties in nature has the following problems: (1) the cycle is long, and it usually takes a long time to find TuMV-resistant varieties in the field; (2) the efficiency is low, and nature Limited availability of resistant cultivars in the

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  • Cultivation and application methods of arabidopsis xpo1a/xpo1b+- double mutant
  • Cultivation and application methods of arabidopsis xpo1a/xpo1b+- double mutant

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Embodiment Construction

[0041] A method for cultivating an Arabidopsis xpo1a / xpo1b double mutant, specifically comprising the following steps:

[0042] (1) Obtain the Arabidopsis thaliana xpo1a mutant (Arabidopsis mutant library number: SALK_078639C);

[0043] (2) 20 days after sowing, DNA and RNA were extracted from the leaves of Arabidopsis thaliana plants of the xpo1a mutant by the CTAB method and the Trizol method;

[0044] (3) Utilize the primer pair LBb1.3-F and m-xpo1a-RP, m-xpo1a-LP and m-xpo1a-RP, and use the extracted DNA as a template for PCR amplification to determine the homozygous situation of the xpo1a mutant (due to T - DNA insertion, LBb1.3-F and m-xpo1a-RP can amplify DNA fragments of specific length, but m-xpo1a-LP and m-xpo1a-RP cannot amplify DNA fragments of specific length). The primer sequences used were:

[0045] LBb1.3-F (tcgcttgtgaatattgtgcag)

[0046] m-xpo1a-LP (atcaaggcagggaaacaaaac)

[0047] m-xpo1a-RP (tgggcagaaatcataggacag); as shown in SEQ ID: No.3-5;

[0048] The...

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Abstract

The invention discloses cultivation and application methods of an arabidopsis xpo1a / xpo1b+- double mutant. The cultivation method comprises crossing an xpo1a mutant and an xpo1b mutant through genetichybridization and screening offspring to obtain the arabidopsis xpo1a / xpo1b+- double mutant. The obtained T3-generation arabidopsis xpo1a / xpo1b+- double mutant plant can significantly impede infection of turnip mosaic virus (TuMV).

Description

technical field [0001] The invention relates to the technical field of Arabidopsis thaliana cultivation, in particular to a method for cultivating Arabidopsis thaliana xpo1a / xpo1b± double mutants. Background technique [0002] Turnip mosaic virus (TuMV) is the main pathogen of virus diseases of cruciferous vegetables and rapeseed in my country. One of the most important plant viruses. At present, the prevention and control of TuMV mainly relies on resistant varieties, but relying on finding resistant varieties in nature has the following problems: (1) the cycle is long, and it usually takes a long time to find TuMV-resistant varieties in the field; (2) the efficiency is low, and nature A limited number of resistant cultivars exist in , severely limiting the use of TuMV-resistant cultivars. Contents of the invention [0003] The object of the present invention is to provide a cultivation method and application of Arabidopsis thaliana xpo1a / xpo1b ± double mutant. [0004] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04C12Q1/6895
CPCA01H1/02A01H1/04C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/158
Inventor 李方方周雪平张明振
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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