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Delftia tsuruhatensis strain H1 with chloroaniline degradation capacity and application thereof

A Delft bacteria, chloroaniline technology, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc. The effect of clear genetic background

Inactive Publication Date: 2011-03-30
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] After retrieving patents and other relevant literature, no report has been found on the application of Delftia tsuruhatensis to degrade chloroaniline under aerobic conditions

Method used

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  • Delftia tsuruhatensis strain H1 with chloroaniline degradation capacity and application thereof
  • Delftia tsuruhatensis strain H1 with chloroaniline degradation capacity and application thereof
  • Delftia tsuruhatensis strain H1 with chloroaniline degradation capacity and application thereof

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Embodiment 1

[0024] Example 1: Isolation, purification and identification of Delftia tsuruhatensis H1 (CCTCC No: M 209249)

[0025] 1. Isolation and purification of Delftia tsuruhatensis H1

[0026] Delftia tsuruhatensis H1 is a gram-negative bacterium obtained by domestication, separation and purification from the activated sludge in the aeration tank of Zhejiang Zhenhai Refining and Chemical Wastewater Treatment Plant. Specific steps are as follows:

[0027] (1) Directed domestication of sludge

[0028] Activated sludge from the aeration tank of Zhejiang Zhenhai Refining and Chemical Wastewater Treatment Plant was taken. In the SBR reactor, the activated sludge is subjected to directional domestication of chloroanilines, and every 36 hours is a domestication cycle, and the domestication lasts for 4 months until the activated sludge achieves stable and efficient degradation of chloroanilines.

[0029] (2) Screening and purification of bacterial strains

[0030] Take out 5mL of sludge ...

Embodiment 2

[0039] Embodiment 2: Degradation characteristics of Delftia tsuruhatensis H1 p-chloroanilines

[0040] 1. Biodegradation of p-chloroaniline by Delftia tsuruhatensis H1 at different temperatures

[0041] The degradation experiment of Delftia tsuruhatensis H1 p-chloroaniline was carried out at different temperatures, and it was found that it has a high ability to degrade chloroaniline mixtures at 25-35 ° C. From the perspective of practical application, 30 ° C is the most suitable temperature. At this time The removal rate is the highest, and the specific implementation steps are as follows:

[0042] In inorganic medium (inorganic salt medium consists of: KH 2 PO 4 , 0.24g / L; Na 2 HPO 4 , 0.24g / L; MgSO 4 ·7H 2 O, 0.0696g / L; CaCl 2 2H 2 O, 0.022g / L; FeCl 3 , 0.005g / L; CuSO 4 ·H 2 O, 0.00003g / L; MnSO 4 ·H 2 O, 0.00013g / L; ZnCl 2 , 0.00023g / L; CoCl 6H 2 O, 0.00042g / L; NaMoO 4 2H 2 O, 0.00015g / L; AlCl 3 ·6H 2 O, 0.00005g / L; solvent is water, pH7~7.5), add chloroan...

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Abstract

The invention provides a delftia tsuruhatensis strain H1 with high efficiency and fast degradation of chloroaniline substances and an application thereof. The delftia tsuruhatensis strain H1 is collected in China Centre for Type Culture Collection, the address is Wuhan University, Wuhan, China, 430072, the collection number CCTCC No. is M 209249, and the collection data is November 4, 2009. The strain can realize fast degradation of chloroaniline pollutants under aerobic conditions, and play an important role in practices of chloroaniline industrial wastewater treatment. In addition, the strain is wild, has a clear genetic background, is applicable to genetic improvement and is expected to greatly improve the capacity of degrading the chloroaniline pollutants.

Description

(1) Technical field [0001] The invention relates to a new bacterial strain with the ability to degrade chloroaniline——Delftia tsuruhatensis strain H1 and its application. (2) Background technology [0002] Chloroaniline compounds are a class of important chemical raw materials, which are widely used in the synthesis of dyes, pesticides, preservatives and pharmaceutical industries. As a family of chlorinated aromatic compounds, they have "three effects" and genotoxicity, are difficult to biodegrade, remain in the environment for a long time, and are easy to bioaccumulate, posing a serious threat to the ecological environment and human health. They have been approved by the US Environmental Protection Agency ( EPA) and the European Union are included in the list of priority pollutants. Therefore, it is of great significance to study the efficient degradation of chloroanilines to improve the quality of water environment and protect human health and ecological security. Biodeg...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/02C02F3/34A62D3/02C12R1/01C02F101/38A62D101/26
CPCY02W10/10
Inventor 张丽丽陈建孟何丹朱润晔杨卫兵
Owner ZHEJIANG UNIV OF TECH
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