Degradation microorganism composition for environment-friendly toilet and application of degradation microorganism composition

A technology of microbial composition and microbial preparation, applied in the field of microbial treatment, can solve the problems of large odor, long treatment time, unsuitable for special operation needs of drilling teams, etc., and achieves simple preparation method, short treatment period and remarkable deodorization effect. Effect

Pending Publication Date: 2022-05-06
SINOPEC SSC +2
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the single strains currently available on the market are mainly strains for urban sewage treatment, which are not suitable for the special operation needs of drilling teams. During use, problems such as strong odor and long treatment time often occur

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Degradation microorganism composition for environment-friendly toilet and application of degradation microorganism composition
  • Degradation microorganism composition for environment-friendly toilet and application of degradation microorganism composition
  • Degradation microorganism composition for environment-friendly toilet and application of degradation microorganism composition

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0049] In the method for preparing the composite microbial bacteria disclosed in the embodiments of the present invention, in order to maximize the mutual benefit and symbiosis synergy between different microorganisms, to realize the rapid and efficient degradation of the composite bacteria of the present invention to feces, during the experiment process. Microbial culture and fermentation conditions and industrial medium have been optimized.

[0050] Preferably, the Trichothecene pink powder is obtained by fermenting and culturing a medium containing thiamine. Preferably, the high-temperature actinomycetes powder is obtained by fermenting culture medium containing malt extract. In a specific embodiment of the present invention, the fermentation medium of described Bacillus subtilis consists of: tryptone 10g, beef extract 30g, sodium chloride 4g, agar 10g, distilled water 1000mL. The fermentation medium of described Aspergillus niger consists of: potato 230g, chopping, add wa...

Embodiment 1

[0073] (1) Inoculate Bacillus subtilis, Aspergillus niger, Trichothecene pink, Lactobacillus brevis and thermoactinomycetes into respective fermentation medium respectively, cultivate them on a shaker, and after centrifugation and conventional freeze-drying, 5 A microbial single-bacteria powder;

[0074] Wherein, the fermentation conditions: fermentation temperature 26°C, inoculum size 8% v / v, stirring speed 100rpm, fermentation time 24h, air is passed into the culture medium during the fermentation process, and the gas-liquid volume ratio of the air per minute is 1.

[0075] The viable count of the Bacillus subtilis powder reaches 2.1×10 8 piece / g;

[0076] The bacterial viability of described Aspergillus niger powder reaches 5.4 * 10 9 piece / g;

[0077] The viable count of the said trichothecene pink mold powder reaches 7.8×10 11 piece / g;

[0078] The viable count of the Lactobacillus brevis powder reaches 6.6×10 8 piece / g;

[0079] The viable count of the high-tempe...

Embodiment 2

[0085] (1) Inoculate Bacillus subtilis, Aspergillus niger, Trichothecene pink, Lactobacillus brevis and thermoactinomycetes in the fermentation medium respectively, cultivate them on a shaker, and after centrifugation and freeze-drying, 5 kinds of microbial single bacteria powder;

[0086] Wherein, the fermentation conditions: fermentation temperature 38°C, inoculum size 16% v / v, stirring speed 200rpm, fermentation time 48h, air is passed into the culture medium during the fermentation process, and the gas-liquid volume ratio of the air per minute is 1.5.

[0087] The viable count of the Bacillus subtilis powder reaches 3.3×10 8 piece / g;

[0088] The bacterial viability of described Aspergillus niger powder reaches 9.2 * 10 9 piece / g;

[0089] The bacterial viability of the said trichothecene pink mold powder reaches 7.9×10 12 piece / g;

[0090] The viable count of the Lactobacillus brevis powder reaches 1.8×10 8 piece / g;

[0091] The viable count of the high-temperatur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a degradation microbial composition for an environment-friendly toilet and application of the degradation microbial composition. The microbial composition comprises bacillus subtilis, aspergillus niger, trichothecium roseum, lactobacillus brevis and thermoactinomycetes. The constructed specific degrading flora is used for treating toilet feces and the like, the symbiotic and synergistic effects of single degrading bacteria can be fully exerted, rapid degradation of protein, starch, cellulose and the like in the feces is achieved, and the method has the advantages of being high in degradation rate, short in treatment period and high in adaptability. According to the embodiment of the invention, 0.03-0.2% (mass-volume ratio) of the degrading bacterium is added into a feces regulating tank, the reaction time is 6-14 hours, the COD (Chemical Oxygen Demand) reduction rate of wastewater reaches 93.9% or above, and the deodorization effect is remarkable; the device is used for treating environment-friendly toilets of drilling crew and has a good application prospect. In addition, the degrading bacteria disclosed by the invention are simple in preparation method, low in cost, high in viable count and suitable for large-scale production and application.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment, and relates to a degradable microbial composition used in an environmentally friendly toilet and its application, in particular to a degradable microbial composition, a microbial preparation and a preparation method and application thereof for an environmentally friendly toilet of a drilling team. Background technique [0002] Carry out green construction in an all-round way for oil exploration, create green projects, create green enterprises, and promote the transformation of dry toilets. Restricted by water sources and drainage conditions, temporary field construction teams such as drilling teams and operation teams still generally use simple dry toilets. The excrement in the septic tanks in the toilets is likely to cause environmental pollution, fail to meet environmental protection discharge requirements, and may also cause diseases. infect. [0003] In order to improve the damag...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/14C02F11/02C12R1/685C12R1/125C12R1/24C12R1/01C12R1/645
CPCC12N1/20C12N1/14C02F11/02
Inventor 单海霞李建亭刘晓宇杨云鹏周启成周亚贤
Owner SINOPEC SSC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap