SNP molecular marker A045736268C/T for identification of Chinese cabbage turnip mosaic virus resistance and application thereof
A technology of turnip mosaic virus and DNA molecules, applied in the biological field, can solve the problem of virus disease without specific chemical control and other problems
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Embodiment 1
[0062] Embodiment 1, the acquisition of SNP molecular marker
[0063] Through genome resequencing data analysis of the highly susceptible turnip mosaic virus strain T12-19 and the highly resistant turnip mosaic virus strain 91-112, it was found that (the male parent 91-112 in the embodiment was continuously selfed for 9 generations The advanced inbred line of Chinese cabbage is highly resistant to TuMV-C4; the female parent T12-19 is a double haploid line and highly susceptible to TuMV-C4. Both the male parent 91-112 and the female parent T12-19 are listed in the literature "Genetic mapping and localization of a major QTL for seedling resistance todowny mildew in Chinese cabbage (Brassica rapa ssp. pekinensis, Mol Breeding (2009) 23: 573–590", publicly available from Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences.), There is a C / T mutation SNP site at base 5736268 of chromosome A04. This SNP site is named A045736268C / T site, which can be used a...
Embodiment 2
[0075] Example 2, Application of Molecular Marker A045736268C / T Site in Identifying Susceptibility to Turnip Mosaic Virus and Resistance to Turnip Mosaic Virus
[0076] 1. Molecular marker identification of materials susceptible to turnip mosaic virus disease and materials resistant to turnip mosaic virus disease
[0077] 1) DNA extraction
[0078] The male parent 91-112 in the examples is a Chinese cabbage high-generation inbred line that has been self-bred for 9 generations and is highly resistant to TuMV-C4; the female parent T12-19 is a double haploid line and is highly susceptible to TuMV-C4. Both the male parent 91-112 and the female parent T12-19 were disclosed in the literature "Geneticmapping and localization of a major QTL for seedling resistance to downymildew in Chinese cabbage (Brassica rapa ssp. pekinensis, Mol Breeding (2009) 23:573-590" However, the public can obtain it from the Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences....
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