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SNP molecular marker A045736268C/T for identification of Chinese cabbage turnip mosaic virus resistance and application thereof

A technology of turnip mosaic virus and DNA molecules, applied in the biological field, can solve the problem of virus disease without specific chemical control and other problems

Active Publication Date: 2018-06-01
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there have been no specific pesticides to prevent and control viral diseases in production, and the main method of prevention and control is to promote disease-resistant varieties combined with cultivation management

Method used

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  • SNP molecular marker A045736268C/T for identification of Chinese cabbage turnip mosaic virus resistance and application thereof
  • SNP molecular marker A045736268C/T for identification of Chinese cabbage turnip mosaic virus resistance and application thereof
  • SNP molecular marker A045736268C/T for identification of Chinese cabbage turnip mosaic virus resistance and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0062] Embodiment 1, the acquisition of SNP molecular marker

[0063] Through genome resequencing data analysis of the highly susceptible turnip mosaic virus strain T12-19 and the highly resistant turnip mosaic virus strain 91-112, it was found that (the male parent 91-112 in the embodiment was continuously selfed for 9 generations The advanced inbred line of Chinese cabbage is highly resistant to TuMV-C4; the female parent T12-19 is a double haploid line and highly susceptible to TuMV-C4. Both the male parent 91-112 and the female parent T12-19 are listed in the literature "Genetic mapping and localization of a major QTL for seedling resistance todowny mildew in Chinese cabbage (Brassica rapa ssp. pekinensis, Mol Breeding (2009) 23: 573–590", publicly available from Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences.), There is a C / T mutation SNP site at base 5736268 of chromosome A04. This SNP site is named A045736268C / T site, which can be used a...

Embodiment 2

[0075] Example 2, Application of Molecular Marker A045736268C / T Site in Identifying Susceptibility to Turnip Mosaic Virus and Resistance to Turnip Mosaic Virus

[0076] 1. Molecular marker identification of materials susceptible to turnip mosaic virus disease and materials resistant to turnip mosaic virus disease

[0077] 1) DNA extraction

[0078] The male parent 91-112 in the examples is a Chinese cabbage high-generation inbred line that has been self-bred for 9 generations and is highly resistant to TuMV-C4; the female parent T12-19 is a double haploid line and is highly susceptible to TuMV-C4. Both the male parent 91-112 and the female parent T12-19 were disclosed in the literature "Geneticmapping and localization of a major QTL for seedling resistance to downymildew in Chinese cabbage (Brassica rapa ssp. pekinensis, Mol Breeding (2009) 23:573-590" However, the public can obtain it from the Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences....

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Abstract

The invention discloses an SNP molecular marker A045736268C / T for identification of Chinese cabbage turnip mosaic virus (TuMV) resistance and application thereof. The invention provides application ofa polymorphic or genotype substance for detecting the A045736268C / T locus in a to-be-detected Chinese cabbage genome to identification or assistant identification of the turnip mosaic virus resistance of the to-be-detected Chinese cabbage. The experiment of the invention utilizes DH population, artificial mechanical inoculation of TuMV at seedling stage is carried out, existing Chinese cabbage genome information is utilized for resistant gene positioning and molecular marker development, preliminary research shows that the Chinese cabbage TuMV resistant gene is located on the A04 chromosome,the developed SNP marker closely linked to the resistant gene has a selection accuracy rate of 100% in both resistant and susceptible materials, and can be used for molecular marker assisted selectivebreeding. The study lays the foundation for further positional cloning and germplasm resource innovation of the Chinese cabbage TuMV resistant gene.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a SNP molecular marker A045736268C / T for identifying Chinese cabbage turnip mosaic virus resistance and an application thereof. Background technique [0002] Virus disease is one of the three major diseases in the production of Chinese cabbage. It can occur throughout the growth period and seriously affects the production of Chinese cabbage. Due to its harm, my country's Chinese cabbage has an average annual output loss of 5%. However, there has been no effective chemical control for viral diseases in production, and the prevention and control is mainly carried out by promoting disease-resistant varieties combined with cultivation management. Screening disease-resistant genetic resources and cultivating new disease-resistant varieties is the fundamental way to solve the viral diseases of Chinese cabbage, and it is also one of the most important breeding goals of Chinese c...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895A01H1/04
CPCA01H1/04C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王姣于拴仓苏同兵张凤兰余阳俊张德双赵岫云汪维红李佩蓉
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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