Hybridoma cell line secreting monoclonal antibody against tomato spotted wilt virus and its application

Abstract

The invention discloses a hybridoma cell strain capable of secreting a tomato spotted wilt virus resistant monoclonal antibody and application of the monoclonal antibody. The preparation method comprises the following steps: immunizing a BALB / c mouse by taking tomato spotted wilt virus particles purified by a differential centrifugation method to serve as an antigen, performing cell fusion, screening and cloning, thereby obtaining a hybridoma cell strain 1A5 capable of performing stable passage and secreting the tomato spotted wilt virus resistant monoclonal antibody, wherein the collection number of the strain is CGMCC No.9343. The monoclonal antibody secreted by the hybridoma cells has the ascitic fluid ELISA titer of 10<-8>, and the type and sub-type of the antibody are IgG1 and kappa chain. A specific reaction is carried out between the monoclonal antibody secreted by 1A5 and the tomato spotted wilt virus only, and the monoclonal antibody does not react with turnip mosaic virus, tomato mosaic virus, iris yellow spot virus and cucumber mosaic virus; and moreover, the monoclonal antibody can be used for specifically and sensitively detecting the tomato spotted wilt virus. The hybridoma cell strain and the secreted monoclonal antibody provide technology and material supports for diagnosis, detection and scientific prevention and control of the tomato virus diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell line secreting anti-tomato spotted wilt virus monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Tomato spotted wilt virus (TSWV) is an important virus that endangers agricultural production. It has a wide host range and can attack 160 species of dicotyledonous plants and 10 species of monocotyledonous plants, especially some plants of Solanaceae, Compositae and Fabaceae. It is widely distributed in many countries and regions such as Europe, North America, South America, Asia and Oceania, and occurs in tropical, subtropical and temperate regions. It belongs to category A2 quarantine pests of the European and Mediterranean Plant Protection Organization (EPPO), and is also a quarantine pest of plants imported into China. [0003] Tomato spotted wilt virus was first discovered in tomato in Australia in 1919, and Brilleleb...

AI Technical Summary

Problems solved by technology

All of these methods have their limitations and are not suitable for the detection of large quantities of field samples

Serological methods are suitable for batch detection of field samples, but must rely on specific virus monoclonal antibodies

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